Upregulation of heme oxygenase‑1 protected against brain damage induced by transient cerebral ischemia‑reperfusion injury in rats

Lu,Xiufang; Gu,Renjun; Hu,Weimin; Sun,Zhitang; Wang,Gaiqing; Wang,Li; Xu,Yuming

doi:10.3892/etm.2018.6049

Upregulation of heme oxygenase‑1 protected against brain damage induced by transient cerebral ischemia‑reperfusion injury in rats

Authors:
- Xiufang Lu
- Renjun Gu
- Weimin Hu
- Zhitang Sun
- Gaiqing Wang
- Li Wang
- Yuming Xu
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Affiliations: Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Department of Neurology, The Second Affiliated Hospital of Xinxiang Medical College, Xinxiang, Henan 453100, P.R. China, Department of Neurology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China
Published online on: April 10, 2018 https://doi.org/10.3892/etm.2018.6049
Pages: 4629-4636
Copyright: © Lu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to identify the effect of heme oxygenase (HO)‑1 gene on cerebral ischemia‑reperfusion injury. Sprague‑Dawley rats were divided randomly into four groups: Sham group, vehicle group, empty adenovirus vector (Ad) group and recombinant HO‑1 adenovirus (Ad‑HO‑1) transfection group. Rats in the vehicle, Ad and Ad‑HO‑1 groups were respectively injected with saline, Ad or Ad‑HO‑1 for 3 days prior to cerebral ischemia‑reperfusion injury. Subsequently, the middle cerebral artery occlusion method was used to establish the model of cerebral ischemia‑reperfusion injury. Following the assessment of neurological function, rats were sacrificed, and the infarction volume and apoptotic index in rat brains were measured. Furthermore, the protein expression levels of HO‑1 in brain tissues were detected using western blot analysis. Results indicated that the neurological score of the Ad‑HO‑1 group was significantly increased compared with the Ad or vehicle groups, respectively (P<0.001). The volume of cerebral infarction and the index score of neuronal apoptosis in the vehicle and Ad groups was significantly increased compared with the Ad‑HO‑1 group (P<0.01). The death of neuronal cells following cerebral ischemia‑reperfusion injury reduced remarkably induced by over‑expression of HO‑1. These findings suggest a neuroprotective role of HO‑1 against brain injury induced by transient cerebral ischemia‑reperfusion injury.

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