Salidroside mediates apoptosis and autophagy inhibition in concanavalin A‑induced liver injury

Feng,Jiao; Niu,Peiqin; Chen,Kan; Wu,Liwei; Liu,Tong; Xu,Shizan; Li,Jingjing; Li,Sainan; Wang,Wenwen; Lu,Xiya; Yu,Qiang; Liu,Ning; Xu,Ling; Wang,Fan; Dai,Weiqi; Xia,Yujing; Fan,Xiaoming; Guo,Chuanyong

doi:10.3892/etm.2018.6053

Salidroside mediates apoptosis and autophagy inhibition in concanavalin A‑induced liver injury

Authors:
- Jiao Feng
- Peiqin Niu
- Kan Chen
- Liwei Wu
- Tong Liu
- Shizan Xu
- Jingjing Li
- Sainan Li
- Wenwen Wang
- Xiya Lu
- Qiang Yu
- Ning Liu
- Ling Xu
- Fan Wang
- Weiqi Dai
- Yujing Xia
- Xiaoming Fan
- Chuanyong Guo
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Affiliations: Department of Gastroenterology, Shanghai Tenth People's Hospital, Affiliated to Tongji University School of Medicine, Shanghai 200072, P.R. China, Department of Gastroenterology, Shanghai Tenth People's Hospital Chongming Branch, Tongji University School of Medicine, Shanghai 202157, P.R. China, School of Clinical Medicine of Nanjing Medical University, Shanghai Tenth People's Hospital, Shanghai 200072, P.R. China, Department of Gastroenterology, Shanghai Tongren Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200336, P.R. China, Department of Oncology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200080, P.R. China, Department of Gastroenterology, Zhongshan Hospital of Fudan University, Shanghai 200032, P.R. China, Department of Gastroenterology, Jinshan Hospital of Fudan University, Shanghai 201508, P.R. China
Published online on: April 12, 2018 https://doi.org/10.3892/etm.2018.6053
Pages: 4599-4614
Copyright: © Feng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Salidroside (Sal) is a glycoside extract from Rhodiola rosea L. with anti‑inflammatory, antioxidant, anticancer and cardioprotective properties. The present study explored the protective effects and the possible mechanisms of Sal on concanavalin A (ConA)‑induced liver injury in mice. Balb/C mice were divided into five groups: Normal control (injected with normal saline), ConA (25 mg/kg), Sal (10 mg/kg) +ConA, Sal (20 mg/kg) + ConA (Sal injected 2 h prior to ConA injection) and Sal (20 mg/kg) only. The serum levels of liver enzymes, pro‑inflammatory cytokines, and apoptosis‑ and autophagy‑associated marker proteins were determined at 2, 8 and 24 h after ConA injection. LY294002 was further used to verify whether the phosphoinositide 3‑kinase (PI3K)/Akt pathway was activated. Primary hepatocytes were isolated to verify the effect of Sal in vitro. The results indicated that Sal was a safe agent to reduce pathological damage and serum liver enzymes in ConA‑induced liver injury. Sal suppressed inflammatory reactions in serum and liver tissues, and activated the PI3K/Akt signaling pathway to inhibit apoptosis and autophagy in vivo and in vitro, which could be reversed by LY294002. In conclusion, Sal attenuated ConA‑induced liver injury by modulating PI3K/Akt pathway‑mediated apoptosis and autophagy in mice.

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