Open Access

Protective effects of human induced pluripotent stem cell‑derived exosomes on high glucose‑induced injury in human endothelial cells

  • Authors:
    • Qianqian Ding
    • Ruiting Sun
    • Pingping Wang
    • Heng Zhang
    • Meng Xiang
    • Dan Meng
    • Ning Sun
    • Alex F. Chen
    • Sifeng Chen
  • View Affiliations

  • Published online on: April 13, 2018     https://doi.org/10.3892/etm.2018.6059
  • Pages: 4791-4797
  • Copyright: © Ding et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Exosomes are a family of extracellular vesicles that are secreted from almost all types of cells and are associated with cell‑to‑cell communication. The present study was performed to investigate the effects of human induced pluripotent stem cell‑derived exosomes (hiPSC‑exo) on cell viability, capillary‑like structure formation and senescence in endothelial cells exposed to high glucose. Exosomes were isolated from the conditional medium of hiPSCs and confirmed by transmission electron microscopy, nanoparticle tracking analysis and western blot analysis using Alix and cluster of differentiation‑63 as markers. hiPSC‑exo were labeled with PKH26 for tracking, and it was determined that spherical exosomes, with a typical cup‑shape, were absorbed by human umbilical vascular endothelial cells (HUVECs). Cultured HUVECs were treated with high glucose (33 mM) with or without hiPSC‑exo (20 µg/ml) for 48 h, and cell viability, capillary tube formation and senescence were assessed. When exposed to high glucose, viability and tube formation in HUVECs was significantly reduced (P<0.0001), whereas the proportion of senescent cells was higher compared with that in control HUVECs (P<0.0001). Furthermore, hiPSC‑exo restored cell viability and capillary‑like structure formation, and reduced senescence in HUVECs exposed to high glucose (P<0.0001). However, hiPSC‑exo had minimal effects on normal HUVECs. These findings suggest that stem cell‑derived exosomes are able to promote cell proliferation, enhance capillary‑like structure formation and reduce senescence in endothelial cells exposed to high glucose.
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June-2018
Volume 15 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Ding Q, Sun R, Wang P, Zhang H, Xiang M, Meng D, Sun N, Chen AF and Chen S: Protective effects of human induced pluripotent stem cell‑derived exosomes on high glucose‑induced injury in human endothelial cells. Exp Ther Med 15: 4791-4797, 2018
APA
Ding, Q., Sun, R., Wang, P., Zhang, H., Xiang, M., Meng, D. ... Chen, S. (2018). Protective effects of human induced pluripotent stem cell‑derived exosomes on high glucose‑induced injury in human endothelial cells. Experimental and Therapeutic Medicine, 15, 4791-4797. https://doi.org/10.3892/etm.2018.6059
MLA
Ding, Q., Sun, R., Wang, P., Zhang, H., Xiang, M., Meng, D., Sun, N., Chen, A. F., Chen, S."Protective effects of human induced pluripotent stem cell‑derived exosomes on high glucose‑induced injury in human endothelial cells". Experimental and Therapeutic Medicine 15.6 (2018): 4791-4797.
Chicago
Ding, Q., Sun, R., Wang, P., Zhang, H., Xiang, M., Meng, D., Sun, N., Chen, A. F., Chen, S."Protective effects of human induced pluripotent stem cell‑derived exosomes on high glucose‑induced injury in human endothelial cells". Experimental and Therapeutic Medicine 15, no. 6 (2018): 4791-4797. https://doi.org/10.3892/etm.2018.6059