Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
June-2018 Volume 15 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
June-2018 Volume 15 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

The effects of doxorubicin‑loaded liposomes on viability, stem cell surface marker expression and secretion of vascular endothelial growth factor of three‑dimensional stem cell spheroids

  • Authors:
    • Hyunjin Lee
    • Jihwan Son
    • Chae‑Bin Na
    • Gawon Yi
    • Heebeom Koo
    • Jun‑Beom Park
  • View Affiliations / Copyright

    Affiliations: Department of Periodontics, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea, Department of Medical Lifescience, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea
    Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4950-4960
    |
    Published online on: April 13, 2018
       https://doi.org/10.3892/etm.2018.6064
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of the present study was to evaluate the effects of anionic, cationic and neutral liposomes containing doxorubicin on the cellular viability and osteogenic differentiation of three‑dimensional stem cell spheroids. Doxorubicin‑loaded liposomes were prepared using the traditional thin‑lipid‑film‑hydration method and were characterized using transmission electron microscopy and a zeta potential analyzer. The doxorubicin release profile from these liposomes was also analyzed in vitro. Three‑dimensional cell spheroids were fabricated using silicon elastomer‑based concave microwells. Qualitative results of cellular viability were observed using a confocal microscope and quantitative cellular viability was evaluated using a Cell‑Counting Kit‑8 (CCK‑8) assay. Furthermore, the secretion of vascular endothelial growth factor was evaluated. Western blot analysis was performed to assess the expression of collagen I and glyceraldehyde 3‑phosphate. Results indicated that the spheroids were well formed in silicon elastomer‑based concave microwells on day 1. In general, the shapes of the cells in the in the doxorubicin‑loaded anionic, cationic and neutral liposome groups were similar to the control group except for the 10 µg/ml groups on days 3, 5, and 7. No significant changes in cellular viability were noted with the addition of doxorubicin at day 1 but significant decreases in cellular viability were noted with application of doxorubicin at day 5. Notably, higher concentrations of doxorubicin reduced the secretion of vascular endothelial growth factor and stem cell marker expression. To conclude, the present study indicated that doxorubicin‑loaded anionic liposomes produced the most sustained release profile and cationic liposomes produced the highest uptake of the stem cell spheroids. These findings suggested that higher concentrations of doxorubicin‑loaded liposomes affected cellular viability, the secretion of vascular endothelial growth factor and stem cell marker expression.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

Abraham SA, Waterhouse DN, Mayer LD, Cullis PR, Madden TD and Bally MB: The liposomal formulation of doxorubicin. Methods Enzymol. 391:71–97. 2005. View Article : Google Scholar : PubMed/NCBI

2 

Soliman A N, Abd-Allah SH, Hussein S and Eldeen Alaa M: Factors enhancing the migration and the homing of mesenchymal stem cells in experimentally induced cardiotoxicity in rats. IUBMB Life. 69:162–169. 2017. View Article : Google Scholar : PubMed/NCBI

3 

Csikos Z, Kerekes K, Fazekas E, Kun S and Borbely J: Biopolymer based nanosystem for doxorubicin targeted delivery. Am J Cancer Res. 7:715–726. 2017.PubMed/NCBI

4 

Fite BZ, Kheirolomoom A, Foiret JL, Seo JW, Mahakian LM, Ingham ES, Tam SM, Borowsky AD, Curry FE and Ferrara KW: Dynamic contrast enhanced MRI detects changes in vascular transport rate constants following treatment with thermally-sensitive liposomal doxorubicin. J Control Release. 256:203–213. 2017. View Article : Google Scholar : PubMed/NCBI

5 

Lee SI, Yeo SI, Kim BB, Ko Y and Park JB: Formation of size-controllable spheroids using gingiva-derived stem cells and concave microwells: Morphology and viability tests. Biomed Rep. 4:97–101. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Wang WZ, Yao XD, Huang XJ, Li JQ and Xu H: Effects of TGF-β1 and alginate on the differentiation of rabbit bone marrow-derived mesenchymal stem cells into a chondrocyte cell lineage. Exp Ther Med. 10:995–1002. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Li Y, Dal-Pra S, Mirotsou M, Jayawardena TM, Hodgkinson CP, Bursac N and Dzau VJ: Tissue-engineered 3-dimensional (3D) microenvironment enhances the direct reprogramming of fibroblasts into cardiomyocytes by microRNAs. Sci Rep. 6:388152016. View Article : Google Scholar : PubMed/NCBI

8 

Lo YP, Liu YS, Rimando MG, Ho JH, Lin KH and Lee OK: Three-dimensional spherical spatial boundary conditions differentially regulate osteogenic differentiation of mesenchymal stromal cells. Sci Rep. 6:212532016. View Article : Google Scholar : PubMed/NCBI

9 

Hsiao C, Tomai M, Glynn J and Palecek SP: Effects of 3D microwell culture on initial fate specification in human embryonic stem cells. AIChE J. 60:1225–1235. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Lee SI, Ko Y and Park JB: Evaluation of the maintenance of stemness, viability and differentiation potential of gingiva-derived stem-cell spheroids. Exp Ther Med. 13:1757–1764. 2017. View Article : Google Scholar : PubMed/NCBI

11 

Bhinge KN, Gupta V, Hosain SB, Satyanarayanajois SD, Meyer SA, Blaylock B, Zhang QJ and Liu YY: The opposite effects of doxorubicin on bone marrow stem cells versus breast cancer stem cells depend on glucosylceramide synthase. Int J Biochem Cell Biol. 44:1770–1778. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Lowery A, Onishko H, Hallahan DE and Han Z: Tumor-targeted delivery of liposome-encapsulated doxorubicin by use of a peptide that selectively binds to irradiated tumors. J Control Release. 150:117–124. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Shah S, Chandra A, Kaur A, Sabnis N, Lacko A, Gryczynski Z, Fudala R and Gryczynski I: Fluorescence properties of doxorubicin in PBS buffer and PVA films. J Photochem Photobiol B. 170:65–69. 2017. View Article : Google Scholar : PubMed/NCBI

14 

Yoo HS, Lee KH, Oh JE and Park TG: In vitro and in vivo anti-tumor activities of nanoparticles based on doxorubicin-PLGA conjugates. J Control Release. 68:419–431. 2000. View Article : Google Scholar : PubMed/NCBI

15 

Lee S, Lee SY, Park S, Ryu JH, Na JH, Koo H, Lee KE, Jeon H, Kwon IC, Kim K and Jeong SY: In vivo NIRF imaging of tumor targetability of nanosized liposomes in tumor-bearing mice. Macromol Biosci. 12:849–856. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Rana T, Chakrabarti A, Freeman M and Biswas S: Doxorubicin-mediated bone loss in breast cancer bone metastases is driven by an interplay between oxidative stress and induction of TGFβ. PLoS One. 8:e780432013. View Article : Google Scholar : PubMed/NCBI

17 

Zhang S, Liu P, Chen L, Wang Y, Wang Z and Zhang B: The effects of spheroid formation of adipose-derived stem cells in a microgravity bioreactor on stemness properties and therapeutic potential. Biomaterials. 41:15–25. 2015. View Article : Google Scholar : PubMed/NCBI

18 

Tang Y, Xu Y, Xiao Z, Zhao Y, Li J, Han S, Chen L, Dai B, Wang L, Chen B and Wang H: The combination of three-dimensional and rotary cell culture system promotes the proliferation and maintains the differentiation potential of rat BMSCs. Sci Rep. 7:1922017. View Article : Google Scholar : PubMed/NCBI

19 

Ylostalo JH, Bazhanov N, Mohammadipoor A and Bartosh TJ: Production and administration of therapeutic mesenchymal stem/stromal cell (MSC) spheroids primed in 3-D cultures under Xeno-free conditions. J Vis Exp. Mar 18–2017. View Article : Google Scholar : PubMed/NCBI

20 

Mohr JC, de Pablo JJ and Palecek SP: 3-D microwell culture of human embryonic stem cells. Biomaterials. 27:6032–6042. 2006. View Article : Google Scholar : PubMed/NCBI

21 

Khademhosseini A, Ferreira L, Blumling J III, Yeh J, Karp JM, Fukuda J and Langer R: Co-culture of human embryonic stem cells with murine embryonic fibroblasts on microwell-patterned substrates. Biomaterials. 27:5968–5977. 2006. View Article : Google Scholar : PubMed/NCBI

22 

Rogozhnikov D, O'Brien PJ, Elahipanah S and Yousaf MN: Scaffold free bio-orthogonal assembly of 3-dimensional cardiac tissue via cell surface engineering. Sci Rep. 6:398062016. View Article : Google Scholar : PubMed/NCBI

23 

Park JB, Zhang H, Lin CY, Chung CP, Byun Y, Park YS and Yang VC: Simvastatin maintains osteoblastic viability while promoting differentiation by partially regulating the expressions of estrogen receptors α. J Surg Res. 174:278–283. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Park JB: Effects of 17-α ethynyl estradiol on proliferation, differentiation & mineralization of osteoprecursor cells. Indian J Med Res. 136:466–470. 2012.PubMed/NCBI

25 

Park JB: Low dose of doxycyline promotes early differentiation of preosteoblasts by partially regulating the expression of estrogen receptors. J Surg Res. 178:737–742. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Barker LP, George KM, Falkow S and Small PL: Differential trafficking of live and dead Mycobacterium marinum organisms in macrophages. Infect Immun. 65:1497–1504. 1997.PubMed/NCBI

27 

Ha DH, Pathak S, Yong CS, Kim JO, Jeong JH and Park JB: Potential differentiation ability of gingiva originated human mesenchymal stem cell in the presence of tacrolimus. Sci Rep. 6:349102016. View Article : Google Scholar : PubMed/NCBI

28 

Almazin SM, Dziak R, Andreana S and Ciancio SG: The effect of doxycycline hyclate, chlorhexidine gluconate and minocycline hydrochloride on osteoblastic proliferation and differentiation in vitro. J Periodontol. 80:999–1005. 2009. View Article : Google Scholar : PubMed/NCBI

29 

Jue SS, Lee WY, Kwon YD, Kim YR, Pae A and Lee B: The effects of enamel matrix derivative on the proliferation and differentiation of human mesenchymal stem cells. Clin Oral Implants Res. 21:741–746. 2010. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Lee H, Son J, Na CB, Yi G, Koo H and Park JB: The effects of doxorubicin‑loaded liposomes on viability, stem cell surface marker expression and secretion of vascular endothelial growth factor of three‑dimensional stem cell spheroids. Exp Ther Med 15: 4950-4960, 2018.
APA
Lee, H., Son, J., Na, C., Yi, G., Koo, H., & Park, J. (2018). The effects of doxorubicin‑loaded liposomes on viability, stem cell surface marker expression and secretion of vascular endothelial growth factor of three‑dimensional stem cell spheroids. Experimental and Therapeutic Medicine, 15, 4950-4960. https://doi.org/10.3892/etm.2018.6064
MLA
Lee, H., Son, J., Na, C., Yi, G., Koo, H., Park, J."The effects of doxorubicin‑loaded liposomes on viability, stem cell surface marker expression and secretion of vascular endothelial growth factor of three‑dimensional stem cell spheroids". Experimental and Therapeutic Medicine 15.6 (2018): 4950-4960.
Chicago
Lee, H., Son, J., Na, C., Yi, G., Koo, H., Park, J."The effects of doxorubicin‑loaded liposomes on viability, stem cell surface marker expression and secretion of vascular endothelial growth factor of three‑dimensional stem cell spheroids". Experimental and Therapeutic Medicine 15, no. 6 (2018): 4950-4960. https://doi.org/10.3892/etm.2018.6064
Copy and paste a formatted citation
x
Spandidos Publications style
Lee H, Son J, Na CB, Yi G, Koo H and Park JB: The effects of doxorubicin‑loaded liposomes on viability, stem cell surface marker expression and secretion of vascular endothelial growth factor of three‑dimensional stem cell spheroids. Exp Ther Med 15: 4950-4960, 2018.
APA
Lee, H., Son, J., Na, C., Yi, G., Koo, H., & Park, J. (2018). The effects of doxorubicin‑loaded liposomes on viability, stem cell surface marker expression and secretion of vascular endothelial growth factor of three‑dimensional stem cell spheroids. Experimental and Therapeutic Medicine, 15, 4950-4960. https://doi.org/10.3892/etm.2018.6064
MLA
Lee, H., Son, J., Na, C., Yi, G., Koo, H., Park, J."The effects of doxorubicin‑loaded liposomes on viability, stem cell surface marker expression and secretion of vascular endothelial growth factor of three‑dimensional stem cell spheroids". Experimental and Therapeutic Medicine 15.6 (2018): 4950-4960.
Chicago
Lee, H., Son, J., Na, C., Yi, G., Koo, H., Park, J."The effects of doxorubicin‑loaded liposomes on viability, stem cell surface marker expression and secretion of vascular endothelial growth factor of three‑dimensional stem cell spheroids". Experimental and Therapeutic Medicine 15, no. 6 (2018): 4950-4960. https://doi.org/10.3892/etm.2018.6064
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team