Open Access

APOE hypermethylation is associated with autism spectrum disorder in a Chinese population

  • Authors:
    • Zhenyu Hu
    • Yong Yang
    • Yuanzhi Zhao
    • Hang Yu
    • Xiuru Ying
    • Dongsheng Zhou
    • Jie Zhong
    • Zhonghua Zheng
    • Jing Liu
    • Ranran Pan
    • Wenwu Zhang
    • Fang Cheng
    • Shiwei Duan
  • View Affiliations

  • Published online on: April 13, 2018     https://doi.org/10.3892/etm.2018.6069
  • Pages: 4749-4754
  • Copyright: © Hu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Abnormal apolipoprotein E (APOE) methylation has been demonstrated to be associated with Alzheimer's disease, which may have overlapping mechanisms with autism spectrum disorder (ASD). Thus, the purpose of the present study was to assess the possible link between APOE methylation and ASD. Genomic DNA was extracted from peripheral blood and subjected to a methylation assay. SYBR green‑based quantitative methylation‑specific polymerase chain reaction analysis was used to measure APOE methylation in 62 pediatric patients with ASD and 73 age‑matched healthy subjects. The APOE methylation in each sample was expressed as a percentage of methylation of a reference (PMR). The results indicated that APOE methylation in pediatric patients with ASD was significantly higher than that in the healthy controls (median PMR, 33 vs. 11%; P=2.36x10‑10). Receiver operating characteristic curve demonstrated that PMR of 15.4% was the optimal cut‑off for predicting ASD (area under curve, 0.817; sensitivity, 93.5%; specificity, 72.6%; P=2.36x10‑10). In summary, the present results indicated that APOE hypermethylation in peripheral blood DNA may be used as a diagnostic biomarker for ASD.
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June-2018
Volume 15 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Hu Z, Yang Y, Zhao Y, Yu H, Ying X, Zhou D, Zhong J, Zheng Z, Liu J, Pan R, Pan R, et al: APOE hypermethylation is associated with autism spectrum disorder in a Chinese population. Exp Ther Med 15: 4749-4754, 2018
APA
Hu, Z., Yang, Y., Zhao, Y., Yu, H., Ying, X., Zhou, D. ... Duan, S. (2018). APOE hypermethylation is associated with autism spectrum disorder in a Chinese population. Experimental and Therapeutic Medicine, 15, 4749-4754. https://doi.org/10.3892/etm.2018.6069
MLA
Hu, Z., Yang, Y., Zhao, Y., Yu, H., Ying, X., Zhou, D., Zhong, J., Zheng, Z., Liu, J., Pan, R., Zhang, W., Cheng, F., Duan, S."APOE hypermethylation is associated with autism spectrum disorder in a Chinese population". Experimental and Therapeutic Medicine 15.6 (2018): 4749-4754.
Chicago
Hu, Z., Yang, Y., Zhao, Y., Yu, H., Ying, X., Zhou, D., Zhong, J., Zheng, Z., Liu, J., Pan, R., Zhang, W., Cheng, F., Duan, S."APOE hypermethylation is associated with autism spectrum disorder in a Chinese population". Experimental and Therapeutic Medicine 15, no. 6 (2018): 4749-4754. https://doi.org/10.3892/etm.2018.6069