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Immunomodulatory effect of Schisandra polysaccharides in cyclophosphamide‑induced immunocompromised mice

  • Authors:
    • Jiahui Yu
    • Lixin Cong
    • Chunmei Wang
    • He Li
    • Chengyi Zhang
    • Xingang Guan
    • Peng Liu
    • Yu Xie
    • Jianguang Chen
    • Jinghui Sun
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, College of Pharmacy, Beihua University, Jilin City, Jilin 132013, P.R. China, Second Treatment Area of Senile Disease, The First Affiliated Hospital of Changchun University of Traditional Chinese Medicine, Changchun, Jilin 130021, P.R. China, Research Center for Life Sciences, Beihua University, Jilin City, Jilin 132013, P.R. China
    Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4755-4762
    |
    Published online on: April 16, 2018
       https://doi.org/10.3892/etm.2018.6073
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Abstract

As a strategy to prevent the well‑known immunosuppressant effects of cyclophosphamide (Cyp), the immunomodulatory effects of the polysaccharide extract of the fruit of Schisandra chinensis (Turcz.) Baill. were investigated in the present study. The crude Schisandra polysaccharide (SCP) was obtained by water extraction and alcohol precipitation methods. The total carbohydrate, uronic acid and protein contents were determined using the phenol‑sulfuric acid, m‑hydroxydiphenyl and Bradford method, respectively. The monosaccharide composition of SCP was determined by high‑performance liquid chromatography. ICR mice were randomly divided into control, model, low‑dose SCP (0.4 mg/10 g), medium‑dose SCP (0.8 mg/10 g) and high‑dose SCP (1.6 mg/10 g) groups. The mice in the SCP groups were intragastrically administered SCP once a day for 21 days and those from the control and model groups were administered the same volume of distilled water. Subsequently, the mice in the model and SCP groups were intraperitoneally injected with Cyp (20 mg/kg) once a day for 5 days. The mouse leukocyte count in the peripheral blood as well as thymus and spleen indexes were determined, and the phagocytic function of macrophages was estimated using a carbon clearance test. The thymus and spleen were histomorphologically observed. The levels of tumor necrosis factor‑α and interferon‑γ were measured by ELISA. Furthermore, antibody formation and spleen lymphocyte proliferation were measured by the serum hemolysin and the MTT method, respectively. The apoptotic rate of splenic lymphocytes was determined by flow cytometric analysis. The results indicated that SCP prevents Cyp‑induced impairment of the cellular, humoral and non‑specific immunity, and may be an auxiliary immune enhancer for the prevention of immune hypofunction.
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1 

Althouse R, Huff J, Tomatis L and Wilbourn J: Chemicals and industrial processes associated with cancer in humans. IARC Monographs Volumes 1 to 20. IARC Monogr Eval Carcinog Risk Chem Hum Suppl. 20:1–71. 1979.

2 

Cheng D, Wan Z, Zhang X, Li J, Li H and Wang C: Dietary Chlorella vulgaris ameliorates altered immunomodulatory functions in cyclophosphamide-induced immunosuppressive mice. Nutrients. 9:pii: E708. 2017. View Article : Google Scholar

3 

Pan G, Xie Z, Huang S, Tai Y, Cai Q, Jiang W, Sun J and Yuan Y: Immune-enhancing effects of polysaccharides extracted from Lilium lancifolium Thunb. Int Immunopharmacol. 52:119–126. 2017. View Article : Google Scholar : PubMed/NCBI

4 

Bo R, Sun Y, Zhou S, Ou N, Gu P, Liu Z, Hu Y, Liu J and Wang D: Simple nanoliposomes encapsulating Lycium barbarum polysaccharides as adjuvants improve humoral and cellular immunity in mice. Int J Nanomedicine. 173:6289–6301. 2017. View Article : Google Scholar

5 

Sheng X, Yan J, Meng Y, Kang Y, Han Z, Tai G, Zhou Y and Cheng H: Immunomodulatory effects of Hericium erinaceus derived polysaccharides are mediated by intestinal immunology. Food Funct. 8:1020–1027. 2017. View Article : Google Scholar : PubMed/NCBI

6 

Panossian A and Wikman G: Pharmacology of Schisandra chinensis Bail: An overview of Russian research and uses in medicine. J Ethnopharmacol. 118:183–212. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Zhao LM, Jia YL, Ma M, Duan YQ and Liu LH: Prevention effects of Schisandra polysaccharide on radiation-induced immune system dysfunction. Int J Biol Macromol. 76:63–69. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Wang E, Chen X, Wang K, Wang J, Chen D, Geng Y, Lai W and Wei X: Plant polysaccharides used as immunostimulants enhance innate immune response and disease resistance against Aeromonas hydrophila infection in fish. Fish Shellfish Immunol. 59:196–202. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Zhao T, Feng Y, Li J, Mao R, Zou Y, Feng W, Zheng D, Wang W, Chen Y, Yang L and Wu X: Schisandra polysaccharide evokes immunomodulatory activity through TLR 4-mediated activation of macrophages. Int J Biol Macromol. 65:33–40. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Nowotny A: Carbohydrate determination by phenol-sulfuric acidBasic Exercises in Immunochemistry. Springer; Berlin: pp. 171–173. 1979, View Article : Google Scholar

11 

Murado MA, Vázquez JA, Montemayor MI, Cabo ML and del Pilar González M: Two mathematical models for the correction of carbohydrate and protein interference in the determination of uronic acids by the m-hydroxydiphenyl method. Biotechnol Appl Biochem. 41:209–216. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 72:248–254. 1976. View Article : Google Scholar : PubMed/NCBI

13 

Zhang X, Yu L, Bi H, Li X, Ni W, Han H, Li N, Wang B, Zhou Y and Tai G: Total fractionation and characterization of the water-soluble polysaccharides isolated from Panax ginseng C. A. Meyer. Carbohyd Polym. 77:544–552. 2009. View Article : Google Scholar

14 

Xu SY, Bian RL and Chen X: Methodology of pharmacological experiment. 3rd edition. Beijing People's Medical Publishing House; pp. 1792002

15 

Jayathirtha MG and Mishra SH: Preliminary immunomodulatory activities of methanol extracts of Eclipta alba and Centella asiatica. Phytomedicine. 11:361–365. 2004. View Article : Google Scholar : PubMed/NCBI

16 

Duan BW, Li Y, Liu X and Yang YJ: Effect of polysaccharides in processed Sibiraea on immunologic function of immunosuppression mice. Zhongguo Zhong Yao Za Zhi. 35:1466–1469. 2010.(In Chinese). PubMed/NCBI

17 

Zdrojewicz Z, Pachura E and Pachura P: The thymus: A forgotten, but very important organ. Adv Clin Exp Med. 25:369–375. 2016. View Article : Google Scholar : PubMed/NCBI

18 

Kraus MD: Splenic histology and histopathology: An update. Semin Diagn Pathol. 20:84–93. 2003. View Article : Google Scholar : PubMed/NCBI

19 

Flomerfelt FA, El Kassar N, Gurunathan C, Chua KS, League SC, Schmitz S, Gershon TR, Kapoor V, Yan XY, Schwartz RH and Gress RE: Tbata modulates thymic stromal cell proliferation and thymus function. J Exp Med. 207:2521–2532. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Lämmermann T and Germain RN: The multiple faces of leukocyte interstitial migration. Semin Immunopathol. 36:227–251. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Zhang PP, Meng ZT, Wang LC, Guo LM and Li K: Astragalus polysaccharide promotes the release of mature granulocytes through the L-selectin signaling pathway. Chin Med. 10:172015. View Article : Google Scholar : PubMed/NCBI

22 

Wynn TA and Vannella KM: Macrophages in tissue repair, regeneration, and fibrosis. Immunity. 44:450–462. 2016. View Article : Google Scholar : PubMed/NCBI

23 

Yu Q, Nie SP, Wang JQ, Yin PF, Huang DF, Li WJ and Xie MY: Toll-like receptor 4-mediated ROS signaling pathway involved in Ganoderma atrum polysaccharide-induced tumor necrosis factor-α secretion during macrophage activation. Food Chem Toxicol. 66:14–22. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Kouakou K, Schepetkin IA, Jun S, Kirpotinal LN, Yapi A, Khramova DS, Pascual DW, Ovodov YS, Jutila MA and Quinn MT: Immunomodulatory activity of polysaccharides isolated from Clerodendrum splendens: Beneficial effects in experimental autoimmune encephalomyelitis. BMC Complement Altern Med. 13:1492013. View Article : Google Scholar : PubMed/NCBI

25 

Yang LC, Lu TJ, Hsieh CC and Lin WC: Characterization and immunomodulatory activity of polysaccharides derived from Dendrobium tosaense. Carbohydr Polym. 111:856–863. 2014. View Article : Google Scholar : PubMed/NCBI

26 

Huang C, Song K, Ma W, Ding J, Chen Z and Zhang M: Immunomodulatory mechanism of Bushen Huoxue Recipe alleviates cyclophosphamide-induced diminished ovarian reserve in mouse model. J Ethnopharmacol. 208:44–56. 2017. View Article : Google Scholar : PubMed/NCBI

27 

Sheng X, Yan J, Meng Y, Kang Y, Han Z, Tai G, Zhou Y and Cheng H: Immunomodulatory effects of Hericium erinaceus derived polysaccharides are mediated by intestinal immunology. Food Funct. 8:1020–1027. 2017. View Article : Google Scholar : PubMed/NCBI

28 

Xing J, Xiao Y, Tang X, Sheng X and Zhan W: Inhibition of Cyclosporine A or rapamycin on T lymphocyte counts and the influence on the immune responses of B lymphocytes in flounder (Paralichthys olivaceus). Fish Shellfish Immunol. 66:78–85. 2017. View Article : Google Scholar : PubMed/NCBI

29 

Liu C, Sun Z, Xu Z, Liu T, Pan T and Li S: Down-regulation of microRNA-155 promotes selenium deficiency-induced apoptosis by tumor necrosis factor receptor superfamily member 1B in the broiler spleen. Oncotarget. 8:58513–58525. 2017.PubMed/NCBI

30 

Pinhu L, Qin Y, Xiong B, You Y, Li J and Sooranna SR: Overexpression of Fas and FasL is associated with infectious complications and severity of experimental severe acute pancreatitis by promoting apoptosis of lymphocytes. Inflammation. 37:1202–1212. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yu J, Cong L, Wang C, Li H, Zhang C, Guan X, Liu P, Xie Y, Chen J, Sun J, Sun J, et al: Immunomodulatory effect of Schisandra polysaccharides in cyclophosphamide‑induced immunocompromised mice. Exp Ther Med 15: 4755-4762, 2018.
APA
Yu, J., Cong, L., Wang, C., Li, H., Zhang, C., Guan, X. ... Sun, J. (2018). Immunomodulatory effect of Schisandra polysaccharides in cyclophosphamide‑induced immunocompromised mice. Experimental and Therapeutic Medicine, 15, 4755-4762. https://doi.org/10.3892/etm.2018.6073
MLA
Yu, J., Cong, L., Wang, C., Li, H., Zhang, C., Guan, X., Liu, P., Xie, Y., Chen, J., Sun, J."Immunomodulatory effect of Schisandra polysaccharides in cyclophosphamide‑induced immunocompromised mice". Experimental and Therapeutic Medicine 15.6 (2018): 4755-4762.
Chicago
Yu, J., Cong, L., Wang, C., Li, H., Zhang, C., Guan, X., Liu, P., Xie, Y., Chen, J., Sun, J."Immunomodulatory effect of Schisandra polysaccharides in cyclophosphamide‑induced immunocompromised mice". Experimental and Therapeutic Medicine 15, no. 6 (2018): 4755-4762. https://doi.org/10.3892/etm.2018.6073
Copy and paste a formatted citation
x
Spandidos Publications style
Yu J, Cong L, Wang C, Li H, Zhang C, Guan X, Liu P, Xie Y, Chen J, Sun J, Sun J, et al: Immunomodulatory effect of Schisandra polysaccharides in cyclophosphamide‑induced immunocompromised mice. Exp Ther Med 15: 4755-4762, 2018.
APA
Yu, J., Cong, L., Wang, C., Li, H., Zhang, C., Guan, X. ... Sun, J. (2018). Immunomodulatory effect of Schisandra polysaccharides in cyclophosphamide‑induced immunocompromised mice. Experimental and Therapeutic Medicine, 15, 4755-4762. https://doi.org/10.3892/etm.2018.6073
MLA
Yu, J., Cong, L., Wang, C., Li, H., Zhang, C., Guan, X., Liu, P., Xie, Y., Chen, J., Sun, J."Immunomodulatory effect of Schisandra polysaccharides in cyclophosphamide‑induced immunocompromised mice". Experimental and Therapeutic Medicine 15.6 (2018): 4755-4762.
Chicago
Yu, J., Cong, L., Wang, C., Li, H., Zhang, C., Guan, X., Liu, P., Xie, Y., Chen, J., Sun, J."Immunomodulatory effect of Schisandra polysaccharides in cyclophosphamide‑induced immunocompromised mice". Experimental and Therapeutic Medicine 15, no. 6 (2018): 4755-4762. https://doi.org/10.3892/etm.2018.6073
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