Open Access

Sub-pathway analysis for severe burns injury patients: Identification of potential key lncRNAs by analyzing lncRNA-mRNA profile

  • Authors:
    • Gongjie Tang
    • Tao Zhang
    • Xinbo Wang
    • Zengmei Song
    • Fucun Liu
    • Qian Zhang
    • Ran Huo
  • View Affiliations

  • Published online on: April 23, 2018     https://doi.org/10.3892/etm.2018.6089
  • Pages: 5281-5287
  • Copyright: © Tang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the study was to identify key long non‑coding RNAs (lncRNA) and related subpathways following severe burn injuries and research their functions. The miRNA-mRNA and lncRNA-miRNA interactions were downloaded from starBase v2.0 database. In addition, mRNA‑miRNA interactions were obtained from TarBase, mirTarBase, mir2Disease, miRecords (V4.0) databases. The relationships of lncRNA‑miRNA‑mRNA were constructed. Genes of expression profiling were intersected with mRNA and lncRNA in lncRNA-mRNA interaction. Screened mRNAs were enriched into various pathways and screened lncRNAs were embedded into candidate pathways. Wallenius approximation methods were used to calculate the false discovery rate value of each sub-pathway. Based on the results of significant sub-pathways, the related lncRNA-mRNA network was constructed. A total of 18,081 genes were obtained. The lncRNA-mRNA intersections including 835 lncRNAs, 1,749 mRNAs and 7,693 interacting pairs were constructed. The enriched mRNAs were further enriched into various candidate pathways such as ribosome biogenesis in eukaryotes. Several sub-pathways were screened, including ribosome biogenesis in eukaryotes and MAPK signaling pathway. The network of pathway-lncRNA-mRNA was constructed. Hub-genes were identified, including C14orf169 and YLPM1. Several hub‑lncRNAs were obtained, including PRKAG2 antisense RNA 1 and LEF1 antisense RNA 1. Several hub-lncRNAs including C14orf169, YLPM1, TTTY15, and PCBP1-AS1 were screened. The sub-pathways regulated by these lncRNAs were identified, and functions were predicted.
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June-2018
Volume 15 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Tang G, Zhang T, Wang X, Song Z, Liu F, Zhang Q and Huo R: Sub-pathway analysis for severe burns injury patients: Identification of potential key lncRNAs by analyzing lncRNA-mRNA profile. Exp Ther Med 15: 5281-5287, 2018
APA
Tang, G., Zhang, T., Wang, X., Song, Z., Liu, F., Zhang, Q., & Huo, R. (2018). Sub-pathway analysis for severe burns injury patients: Identification of potential key lncRNAs by analyzing lncRNA-mRNA profile. Experimental and Therapeutic Medicine, 15, 5281-5287. https://doi.org/10.3892/etm.2018.6089
MLA
Tang, G., Zhang, T., Wang, X., Song, Z., Liu, F., Zhang, Q., Huo, R."Sub-pathway analysis for severe burns injury patients: Identification of potential key lncRNAs by analyzing lncRNA-mRNA profile". Experimental and Therapeutic Medicine 15.6 (2018): 5281-5287.
Chicago
Tang, G., Zhang, T., Wang, X., Song, Z., Liu, F., Zhang, Q., Huo, R."Sub-pathway analysis for severe burns injury patients: Identification of potential key lncRNAs by analyzing lncRNA-mRNA profile". Experimental and Therapeutic Medicine 15, no. 6 (2018): 5281-5287. https://doi.org/10.3892/etm.2018.6089