Sparstolonin B improves neurological outcomes following intracerebral hemorrhage in mice

Wang,Yanchun; Jiang,Side; Xiao,Jing; Liang,Qiaoli; Tang,Mingshan

doi:10.3892/etm.2018.6092

Sparstolonin B improves neurological outcomes following intracerebral hemorrhage in mice

Authors:
- Yanchun Wang
- Side Jiang
- Jing Xiao
- Qiaoli Liang
- Mingshan Tang
View Affiliations

Affiliations: Department of Neurology, Ba‑Nan People's Hospital, Chongqing 401320, P.R. China, School of Pharmacy, Nanjing University of Chinese Medicine, Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing, Jiangsu 210023, P.R. China
Published online on: April 24, 2018 https://doi.org/10.3892/etm.2018.6092
Pages: 5436-5442

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Inflammation serves an important role in inducing secondary injury following intracerebral hemorrhage (ICH). It has been demonstrated that sparstolonin B (SsnB) is able to attenuate the lipopolysaccharide‑induced inflammatory response in sepsis. Mouse ICH models were used to explore the efficacy of SsnB on the ICH‑induced inflammatory response. Mice underwent a working memory version of Morris water maze (MWM) test. They underwent 5 successive days of training consisting of 4 trials each day. The ICH model was established on the last training day. Mice were injected intraperitoneally either with vehicle or SsnB once a day for 3 consecutive days following the establishment of the ICH model. The MWM was used to determine the effect of SsnB on short‑term memory following ICH. Neurological deficit scores and brain water content were measured following the MWM. Furthermore, the expression of inflammatory factors and signaling molecules downstream of TLR4 were measured. The results demonstrated that 5 mg/kg SsnB significantly improved the MWM path and time latency (P<0.05). Furthermore, neurological deficit scores were decreased in SsnB‑treated mice compared with vehicle‑treated mice (P<0.01). Brain water content, levels of inflammatory cytokines and the expression of inflammation‑associated proteins were also significantly reduced in the SsnB‑treated group (P<0.05). These results indicate that SsnB treatment stimulates short‑term neurobehavioral recovery and reduces neurological deficits and this may inhibit the inflammatory response. Therefore, SsnB may attenuate the inflammatory response following ICH.

View Figures

View References

1	Qureshi AI, Mendelow AD and Hanley DF: Intracerebral haemorrhage. Lancet. 373:1632–1644. 2009. View Article : Google Scholar : PubMed/NCBI
2	van Asch CJ, Luitse MJ, Rinkel GJ, van der Tweel I, Algra A and Klijn CJ: Incidence, case fatality, and functional outcome of intracerebral haemorrhage over time, according to age, sex, and ethnic origin: A systematic review and meta-analysis. Lancet Neurol. 9:167–176. 2010. View Article : Google Scholar : PubMed/NCBI
3	Zahuranec DB, Lisabeth LD, Sánchez BN, Smith MA, Brown DL, Garcia NM, Skolarus LE, Meurer WJ, Burke JF, Adelman EE and Morgenstern LB: Intracerebral hemorrhage mortality is not changing despite declining incidence. Neurology. 82:2180–2186. 2014. View Article : Google Scholar : PubMed/NCBI
4	Xi G, Strahle J, Hua Y and Keep RF: Progress in translational research on intracerebral hemorrhage: Is there an end in sight? Prog Neurobiol. 115:45–63. 2014. View Article : Google Scholar : PubMed/NCBI
5	Zhou Y, Wang Y, Wang J, Stetler Anne R and Yang QW: Inflammation in intracerebral hemorrhage: From mechanisms to clinical translation. Prog Neurobiol. 115:25–44. 2014. View Article : Google Scholar : PubMed/NCBI
6	Mendelow AD, Gregson BA, Fernandes HM, Murray GD, Teasdale GM, Hope DT, Karimi A, Shaw MD and Barer DH: STICH investigators: Early surgery versus initial conservative treatment in patients with spontaneous supratentorial intracerebral haematomas in the International Surgical Trial in Intracerebral Haemorrhage (STICH): A randomised trial. Lancet. 365:387–397. 2005. View Article : Google Scholar : PubMed/NCBI
7	Aronowski J and Zhao X: Molecular pathophysiology of cerebral hemorrhage: Secondary brain injury. Stroke. 42:1781–1786. 2011. View Article : Google Scholar : PubMed/NCBI
8	Wang J: Preclinical and clinical research on inflammation after intracerebral hemorrhage. Prog Neurobiol. 92:463–477. 2010. View Article : Google Scholar : PubMed/NCBI
9	Akira S, Uematsu S and Takeuchi O: Pathogen recognition and innate immunity. Cell. 124:783–801. 2006. View Article : Google Scholar : PubMed/NCBI
10	Kong Y and Le Y: Toll-like receptors in inflammation of the central nervous system. Int Immunopharmacol. 11:1407–1414. 2011. View Article : Google Scholar : PubMed/NCBI
11	Wang YC, Zhou Y, Fang H, Lin S, Wang PF, Xiong RP, Chen J, Xiong XY, Lv FL, Liang QL and Yang QW: Toll-like receptor 2/4 heterodimer mediates inflammatory injury in intracerebral hemorrhage. Ann Neurol. 75:876–889. 2014. View Article : Google Scholar : PubMed/NCBI
12	Zhang S, Zou J, Li P, Zheng X and Feng D: Curcumin protects against atherosclerosis in apolipoprotein E-knockout mice by inhibiting toll-like receptor 4. expression. J Agr Food Chem. 66:449–456. 2018. View Article : Google Scholar
13	Yao L, Shi Y, Zhao X, Hou A, Xing Y, Fu J and Xue X: Vitamin D attenuates hyperoxia-induced lung injury through downregulation of Toll-like receptor 4. Int J Mol Med. 39:1403–1408. 2017. View Article : Google Scholar : PubMed/NCBI
14	Liang Q, Wu Q, Jiang J, Duan J, Wang C, Smith MD, Lu H, Wang Q, Nagarkatti P and Fan D: Characterization of sparstolonin B, a Chinese herb-derived compound, as a selective Toll-like receptor antagonist with potent anti-inflammatory properties. J Biol Chem. 286:26470–26479. 2011. View Article : Google Scholar : PubMed/NCBI
15	Liang QL, Lei LL, Cui X, Zou NS and Duan JA: Bioactive cis-stilbenoids from the tubers of Scirpus yagara. Fitoterapia. 84:170–173. 2013. View Article : Google Scholar : PubMed/NCBI
16	Zhong Q, Zhou K, Liang QL, Lin S, Wang YC, Xiong XY, Meng ZY, Zhao T, Zhu WY, Yang YR, et al: Interleukin-23 secreted by activated macrophages drives γδT cell production of interleukin-17 to aggravate secondary injury after intracerebral hemorrhage. J Am Heart Assoc. 5:e0043402016. View Article : Google Scholar : PubMed/NCBI
17	Kilkenny C, Browne WJ, Cuthill IC, Emerson M and Altman DG: Improving bioscience research reporting: The ARRIVE guidelines for reporting animal research. PLoS Biol. 8:e10004122010. View Article : Google Scholar : PubMed/NCBI
18	Vorhees CV and Williams MT: Value of water mazes for assessing spatial and egocentric learning and memory in rodent basic research and regulatory studies. Neurotoxicol Teratol. 45:75–90. 2014. View Article : Google Scholar : PubMed/NCBI
19	Terry AV Jr: Spatial navigation (Water Maze) tasksMethods of behavior analysis in neuroscience. 2nd edition. Buccafusco JJ: Boca Raton (FL): 2009
20	Wang YC, Wang PF, Fang H, Chen J, Xiong XY and Yang QW: Toll-like receptor 4 antagonist attenuates intracerebral hemorrhage-induced brain injury. Stroke. 44:2545–2552. 2013. View Article : Google Scholar : PubMed/NCBI
21	Zhao X, Zhang Y, Strong R, Grotta JC and Aronowski J: 15d-Prostaglandin J2 activates peroxisome proliferator-activated receptor-gamma, promotes expression of catalase, and reduces inflammation, behavioral dysfunction, and neuronal loss after intracerebral hemorrhage in rats. J Cereb Blood Flow Metab. 26:811–820. 2006. View Article : Google Scholar : PubMed/NCBI
22	Lin S, Yin Q, Zhong Q, Lv FL, Zhou Y, Li JQ, Wang JZ, Su BY and Yang QW: Heme activates TLR4-mediated inflammatory injury via MyD88/TRIF signaling pathway in intracerebral hemorrhage. J Neuroinflamm. 9:462012. View Article : Google Scholar
23	Chang CF, Chen SF, Lee TS, Lee HF and Shyue SK: Caveolin-1 deletion reduces early brain injury after experimental intracerebral hemorrhage. Am J Pathol. 178:1749–1761. 2011. View Article : Google Scholar : PubMed/NCBI
24	Belur PK, Chang JJ, He S, Emanuel BA and Mack WJ: Emerging experimental therapies for intracerebral hemorrhage: Targeting mechanisms of secondary brain injury. Neurosurg Focus. 34:E92013. View Article : Google Scholar : PubMed/NCBI
25	Ziai WC: Hematology and inflammatory signaling of intracerebral hemorrhage. Stroke. 44 6 Suppl 1:S74–S78. 2013. View Article : Google Scholar : PubMed/NCBI
26	Liang Q, Dong S, Lei L, Liu J, Zhang J, Li J, Duan J and Fan D: Protective effects of Sparstolonin B, a selective TLR2 and TLR4 antagonist, on mouse endotoxin shock. Cytokine. 75:302–309. 2015. View Article : Google Scholar : PubMed/NCBI
27	Liu Q, Wang J, Liang Q, Wang D, Luo Y, Li J, Janicki JS and Fan D: Sparstolonin B attenuates hypoxia-reoxygenation-induced cardiomyocyte inflammation. Exp Biol Med (Maywood). 239:376–384. 2014. View Article : Google Scholar : PubMed/NCBI
28	Cao Y, Guan K, He X, Wei CW, Zheng ZR, Zhang YH, Ma SL, Zhong H and Shi W: Yersinia YopJ negatively regulates IRF3-mediated antibacterial response through disruption of STING-mediated cytosolic DNA signaling. Biochim Biophys Acta. 1863:3148–3159. 2016. View Article : Google Scholar : PubMed/NCBI