Antinociceptive effects of dezocine on complete Freund's adjuvant‑induced inflammatory pain in rats

Ye,Zhenhai; Zhang,Maoxian; Ding,Ning; Gao,Peng; Hei,Yunpeng; Wang,Yun; Gao,Wei; Ye,Qingshan

doi:10.3892/etm.2018.6110

June-2018 Volume 15 Issue 6

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June-2018 Volume 15 Issue 6

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Article

Antinociceptive effects of dezocine on complete Freund's adjuvant‑induced inflammatory pain in rats

Authors:
- Zhenhai Ye
- Maoxian Zhang
- Ning Ding
- Peng Gao
- Yunpeng Hei
- Yun Wang
- Wei Gao
- Qingshan Ye
View Affiliations / Copyright

Affiliations: Department of Anesthesiology, Ningxia People's Hospital, Yinchuan, Ningxia 750002, P.R. China
Pages: 5469-5474
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Published online on: April 30, 2018

https://doi.org/10.3892/etm.2018.6110
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Abstract

Inflammatory pain is known to severely impact the life quality of patients. Notably, dezocine is widely used for the treatment of pain. Therefore, the current study aimed to examine the effects of dezocine on a complete Freund's adjuvant (CFA)‑induced inflammatory pain model in rats and to investigate the possible underlying molecular mechanisms. Rats were randomly divided into three groups, including the control, CFA and dezocine+CFA groups, and then subcutaneously injected with 100 µl saline, subcutaneously injected with 100 µl CFA or pretreated with 1 ml dezocine (0.4 µg/kg) at 30 min before CFA injection in the plantar surface of right hind paw, respectively. The paw withdrawal threshold (PWT) and paw withdrawal latency (PWL) were measured with a dynamic plantar esthesiometer at 1 day before and 6 h after CFA injection. The ipsilateral lumbar spinal cords of all the rats were harvested for detecting the expression profiles of phosphorylated (p)‑p65, p‑extracellular signal‑regulated kinase 1/2 (p‑ERK1/2), cyclooxygenase‑2 (COX‑2), interleukin (IL)‑1β and tumor necrosis factor (TNF)‑α by western blot analysis and/or reverse transcription‑quantitative polymerase chain reaction. In addition, prostaglandin E2 (PGE2) expression was determined by enzyme‑linked immunosorbent assay. Compared with the control group, CFA‑induced peripheral inflammation downregulated the PWT and PWL values of rats, which were significantly alleviated by dezocine treatment. Furthermore, the protein levels of p‑p65, p‑ERK1/2, COX‑2, PGE2, IL‑1β and TNF‑α were significantly upregulated following CFA injection, while they were suppressed by dezocine pretreatment. In conclusion, the analgesic effect of dezocine on inflammatory pain induced by CFA may be associated with the inhibition of the spinal ERK1/2‑COX‑2 pathway.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Antinociceptive effects of dezocine on complete Freund's adjuvant‑induced inflammatory pain in rats

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