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Minimally effective concentration of zoledronic acid to suppress osteoclasts in vitro

  • Authors:
    • Pengfei Li
    • Zongmao Zhao
    • Litao Wang
    • Xianhui Jin
    • Yaxin Shen
    • Chengrui Nan
    • Hanjie Liu
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China, Department of Bone Disease, Harrison International Peace Hospital, Hengshui, Hebei 053000, P.R. China, Department of Surgery, Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5330-5336
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    Published online on: May 2, 2018
       https://doi.org/10.3892/etm.2018.6120
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Abstract

Zoledronic acid is regarded as the most potent bisphosphonate and is widely used in patients with osteoporosis; however, its side effects, including acute‑phase reactions, gastrointestinal complaints, renal dysfunction and bisphosphonate‑associated osteonecrosis impair the safety and quality of life of patients. The present study was designed to determine the minimal effective concentration of zoledronic acid through testing the dose‑dependent effects of zoledronic acid on osteoclast suppression. A primary culture of bone marrow mononuclear cells obtained from C57 mice (age, 6 weeks) was established and induced to form osteoclasts. The number of multinuclear cells was determined by tartrate‑resistant acid phosphatase staining and compared among cultured marrow cells treated with different concentrations of zoledronic acid. Furthermore, the cellular properties, including adhesion, migration and bone resorption, were compared at the minimal effective concentration. At a concentration of 1x10‑6 mol/l, zoledronic acid significantly inhibited the formation of osteoclasts. This inhibitory effect was further enhanced at the concentration of 1x10‑5 mol/l. However, the inhibitory effect of zoledronic acid tapered at the concentration of 1x10‑4 mol/l and there was no further dose‑dependent increase. In addition, the concentration of 1x10‑6 mol/l was sufficient to alter cellular functions, including cell adhesion, migration and bone resorption. In conclusion, zoledronic acid was effective in reducing osteoclast formation and suppressing cellular functions. The minimal effective concentration of zoledronic acid in vitro was 1 µmol/l. Based on these results, a comparable dosage should be explored in clinical applications.
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Copy and paste a formatted citation
Spandidos Publications style
Li P, Zhao Z, Wang L, Jin X, Shen Y, Nan C and Liu H: Minimally effective concentration of zoledronic acid to suppress osteoclasts in vitro. Exp Ther Med 15: 5330-5336, 2018.
APA
Li, P., Zhao, Z., Wang, L., Jin, X., Shen, Y., Nan, C., & Liu, H. (2018). Minimally effective concentration of zoledronic acid to suppress osteoclasts in vitro. Experimental and Therapeutic Medicine, 15, 5330-5336. https://doi.org/10.3892/etm.2018.6120
MLA
Li, P., Zhao, Z., Wang, L., Jin, X., Shen, Y., Nan, C., Liu, H."Minimally effective concentration of zoledronic acid to suppress osteoclasts in vitro". Experimental and Therapeutic Medicine 15.6 (2018): 5330-5336.
Chicago
Li, P., Zhao, Z., Wang, L., Jin, X., Shen, Y., Nan, C., Liu, H."Minimally effective concentration of zoledronic acid to suppress osteoclasts in vitro". Experimental and Therapeutic Medicine 15, no. 6 (2018): 5330-5336. https://doi.org/10.3892/etm.2018.6120
Copy and paste a formatted citation
x
Spandidos Publications style
Li P, Zhao Z, Wang L, Jin X, Shen Y, Nan C and Liu H: Minimally effective concentration of zoledronic acid to suppress osteoclasts in vitro. Exp Ther Med 15: 5330-5336, 2018.
APA
Li, P., Zhao, Z., Wang, L., Jin, X., Shen, Y., Nan, C., & Liu, H. (2018). Minimally effective concentration of zoledronic acid to suppress osteoclasts in vitro. Experimental and Therapeutic Medicine, 15, 5330-5336. https://doi.org/10.3892/etm.2018.6120
MLA
Li, P., Zhao, Z., Wang, L., Jin, X., Shen, Y., Nan, C., Liu, H."Minimally effective concentration of zoledronic acid to suppress osteoclasts in vitro". Experimental and Therapeutic Medicine 15.6 (2018): 5330-5336.
Chicago
Li, P., Zhao, Z., Wang, L., Jin, X., Shen, Y., Nan, C., Liu, H."Minimally effective concentration of zoledronic acid to suppress osteoclasts in vitro". Experimental and Therapeutic Medicine 15, no. 6 (2018): 5330-5336. https://doi.org/10.3892/etm.2018.6120
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