Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
June-2018 Volume 15 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
June-2018 Volume 15 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Icariin regulates the osteoblast differentiation and cell proliferation of MC3T3‑E1 cells through microRNA‑153 by targeting Runt‑related transcription factor 2

  • Authors:
    • Zengfa Huang
    • Cheng Cheng
    • Jing Wang
    • Xianzhe Liu
    • Hui Wei
    • Yu Han
    • Shuhua Yang
    • Xiang Wang
  • View Affiliations / Copyright

    Affiliations: Department of Radiology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430014, P.R. China, Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China
    Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5159-5166
    |
    Published online on: May 3, 2018
       https://doi.org/10.3892/etm.2018.6127
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Osteoporosis has become one of the most serious public health problems. Icariin, miR‑153 and Runt‑related transcription factor 2 (Runx2) have been demonstrated to regulate cell proliferation and differentiation in multiple cells. The aim of the present experiments was to investigate the potential mechanism underlying osteoblast differentiation and cell proliferation of MC3T3‑E1 cells treated with icariin. Cell Counting kit‑8, alkaline phosphatase (ALP) activity and alizarin red S assays, as well as reverse transcription‑quantitative polymerase chain reaction and western blot analysis, were performed to examine whether icariin promoted osteoblast differentiation and cell proliferation in MC3T3‑E1 cells. Subsequently, miR‑153 target and pathway prediction, and functional analysis were assessed. The results demonstrated that icariin promoted proliferation, mineral content and ALP activity in MC3T3‑E1 cells. In addition, miR‑153 and Runx2 expression levels were increased following treatment with icariin. Luciferase assay revealed that miR‑153 significantly upregulate the luciferase activity of wild‑type (Wt) Runx2 3'‑untranslated region. Furthermore, the group treated with a combination of miR‑153 mimics and icariin exhibited a significantly higher expression of Runx2 in comparison with the miR‑153 mimic‑treated alone group. Finally, icariin reversed the potential effect of miR‑153 inhibitor in MC3T3‑E1 cells. In conclusion, icariin exerted a strong osteoblast differentiation effect in MC3T3‑E1 cells through the miR‑153/Runx2 pathway. The current study provided evidence suggesting that icariin should be considered as an effective candidate for the management of osteoporosis.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Zhao LJ, Liu XG, Liu YZ, Liu YJ, Papasian CJ, Sha BY, Pan F, Guo YF, Wang L, Yan H, et al: Genome-wide association study for femoral neck bone geometry. J Bone Miner Res. 25:320–329. 2010. View Article : Google Scholar : PubMed/NCBI

2 

An J, Yang H, Zhang Q, Liu C, Zhao J, Zhang L and Chen B: Natural products for treatment of osteoporosis: The effects and mechanisms on promoting osteoblast-mediated bone formation. Life Sci. 147:46–58. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Liu YJ, Shen H, Xiao P, Xiong DH, Li LH, Recker RR and Deng HW: Molecular genetic studies of gene identification for osteoporosis: A 2004 update. J Bone Miner Res. 21:1511–1535. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Piscitelli P, Iolascon G, Gimigliano F, Muratore M, Camboa P, Borgia O, Forcina B, Fitto F, Robaud V, Termini G, et al: Incidence and costs of hip fractures compared to acute myocardial infarction in the Italian population: A 4-year survey. Osteoporos Int. 18:211–219. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Reginster JY and Burlet N: Osteoporosis: A still increasing prevalence. Bone. 38 2 Suppl 1:4–9. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Budhia S, Mikyas Y, Tang M and Badamgarav E: Osteoporotic fractures: A systematic review of U.S. healthcare costs and resource utilization. Pharmacoeconomics. 30:147–170. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Yang Y, Du F, Ye W, Chen Y, Li J, Zhang J, Nicely H and Burge R: Inpatient cost of treating osteoporotic fractures in mainland China: A descriptive analysis. Clinicoecon Outcomes Res. 7:205–212. 2015.PubMed/NCBI

8 

Chan DC, Lee YS, Wu YJ, Tsou HH, Chen CT, Hwang JS, Tsai KS and Yang RS: A 12-year ecological study of hip fracture rates among older Taiwanese adults. Calcif Tissue Int. 93:397–404. 2013. View Article : Google Scholar : PubMed/NCBI

9 

Melton LJ 3rd, Chrischilles EA, Cooper C, Lane AW and Riggs BL: Perspective. How many women have osteoporosis? J Bone Miner Res. 7:1005–1010. 1992. View Article : Google Scholar : PubMed/NCBI

10 

Burge R, Dawson-Hughes B, Solomon DH, Wong JB, King A and Tosteson A: Incidence and economic burden of osteoporosis-related fractures in the United States, 2005–2025. J Bone Miner Res. 22:465–475. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Ahmed FE, Ahmed NC, Vos PW, Bonnerup C, Atkins JN, Casey M, Nuovo GJ, Naziri W, Wiley JE, Mota H, et al: Diagnostic microRNA markers to screen for sporadic human colon cancer in stool: I. Proof of principle. Cancer Genomics Proteomics. 10:93–113. 2013.PubMed/NCBI

12 

van Wijnen AJ, van de Peppel J, van Leeuwen JP, Lian JB, Stein GS, Westendorf JJ, Oursler MJ, Im HJ, Taipaleenmäki H, Hesse E, Riester S, et al: MicroRNA functions in osteogenesis and dysfunctions in osteoporosis. Curr Osteoporos Rep. 11:72–82. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Yamasaki K, Nakasa T, Miyaki S, Yamasaki T, Yasunaga Y and Ochi M: Angiogenic microRNA-210 is present in cells surrounding osteonecrosis. J Orthop Res. 30:1263–1270. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Ukai T, Sato M, Akutsu H, Umezawa A and Mochida J: MicroRNA-199a-3p, microRNA-193b, and microRNA-320c are correlated to aging and regulate human cartilage metabolism. J Orthop Res. 30:1915–1922. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Lagos-Quintana M, Rauhut R, Lendeckel W and Tuschl T: Identification of novel genes coding for small expressed RNAs. Science. 294:853–858. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Lau NC, Lim LP, Weinstein EG and Bartel DP: An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans. Science. 294:858–862. 2001. View Article : Google Scholar : PubMed/NCBI

17 

Lee RC and Ambros V: An extensive class of small RNAs in Caenorhabditis elegans. Science. 294:862–864. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Duan Z, Choy E, Nielsen GP, Rosenberg A, Iafrate J, Yang C, Schwab J, Mankin H, Xavier R and Hornicek FJ: Differential expression of microRNA (miRNA) in chordoma reveals a role for miRNA-1 in Met expression. J Orthop Res. 28:746–752. 2010.PubMed/NCBI

19 

Dong S, Yang B, Guo H and Kang F: MicroRNAs regulate osteogenesis and chondrogenesis. Biochem Biophys Res Commun. 418:587–591. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Li H, Xie H, Liu W, Hu R, Huang B, Tan YF, Xu K, Sheng ZF, Zhou HD, Wu XP, et al: A novel microRNA targeting HDAC5 regulates osteoblast differentiation in mice and contributes to primary osteoporosis in humans. J Clin Invest. 119:3666–3677. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Guo J, Ren F, Wang Y, Li S, Gao Z, Wang X, Ning H, Wu J, Li Y, Wang Z, et al: miR-764-5p promotes osteoblast differentiation through inhibition of CHIP/STUB1 expression. J Bone Miner Res. 27:1607–1618. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Gao J, Yang T, Han J, Yan K, Qiu X, Zhou Y, Fan Q and Ma B: MicroRNA expression during osteogenic differentiation of human multipotent mesenchymal stromal cells from bone marrow. J Cell Biochem. 112:1844–1856. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Niu G, Li B, Sun L and An C: MicroRNA-153 inhibits osteosarcoma cells proliferation and invasion by targeting TGF-β2. PLoS One. 10:e01192252015. View Article : Google Scholar : PubMed/NCBI

24 

Wu Z, He B, He J and Mao X: Upregulation of miR-153 promotes cell proliferation via downregulation of the PTEN tumor suppressor gene in human prostate cancer. Prostate. 73:596–604. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Xie L, Zhang Z, Tan Z, He R, Zeng X, Xie Y, Li S, Tang G, Tang H and He X: MicroRNA-124 inhibits proliferation and induces apoptosis by directly repressing EZH2 in gastric cancer. Mol Cell Biochem. 392:153–159. 2014. View Article : Google Scholar : PubMed/NCBI

26 

Song L, Duan P, Guo P, Li D, Li S, Xu Y and Zhou Q: Downregulation of miR-223 and miR-153 mediates mechanical stretch-stimulated proliferation of venous smooth muscle cells via activation of the insulin-like growth factor-1 receptor. Arch Biochem Biophys. 528:204–211. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Ming LG, Chen KM and Xian CJ: Functions and action mechanisms of flavonoids genistein and icariin in regulating bone remodeling. J Cell Physiol. 228:513–521. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Huang J, Yuan L, Wang X, Zhang TL and Wang K: Icaritin and its glycosides enhance osteoblastic, but suppress osteoclastic, differentiation and activity in vitro. Life Sci. 81:832–840. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Nian H, Ma MH, Nian SS and Xu LL: Antiosteoporotic activity of icariin in ovariectomized rats. Phytomedicine. 16:320–326. 2009. View Article : Google Scholar : PubMed/NCBI

30 

Zhao J, Ohba S, Shinkai M, Chung UI and Nagamune T: Icariin induces osteogenic differentiation in vitro in a BMP- and Runx2-dependent manner. Biochem Biophys Res Commun. 369:444–448. 2008. View Article : Google Scholar : PubMed/NCBI

31 

Nash LA, Peters SJ, Sullivan PJ and Ward WE: Supraphysiological levels of quercetin glycosides are required to alter mineralization in Saos2 cells. Int J Environ Res Public Health. 13:2016. View Article : Google Scholar : PubMed/NCBI

32 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

33 

Mok SK, Chen WF, Lai WP, Leung PC, Wang XL, Yao XS and Wong MS: Icariin protects against bone loss induced by oestrogen deficiency and activates oestrogen receptor-dependent osteoblastic functions in UMR 106 cells. Br J Pharmacol. 159:939–949. 2010. View Article : Google Scholar : PubMed/NCBI

34 

Ma HP, Ming LG, Ge BF, Zhai YK, Song P, Xian CJ and Chen KM: Icariin is more potent than genistein in promoting osteoblast differentiation and mineralization in vitro. J Cell Biochem. 112:916–923. 2011. View Article : Google Scholar : PubMed/NCBI

35 

Zhang D, Fong C, Jia Z, Cui L, Yao X and Yang M: Icariin stimulates differentiation and suppresses adipocytic transdifferentiation of primary osteoblasts through estrogen receptor-mediated pathway. Calcif Tissue Int. 187–198. 2016. View Article : Google Scholar : PubMed/NCBI

36 

Li GW, Xu Z, Chang SX, Nian H, Wang XY and Qin LD: Icariin prevents ovariectomy-induced bone loss and lowers marrow adipogenesis. Menopause. 21:1007–1016. 2014. View Article : Google Scholar : PubMed/NCBI

37 

Meng YB, Li X, Li ZY, Zhao J, Yuan XB, Ren Y, Cui ZD, Liu YD and Yang XJ: microRNA-21 promotes osteogenic differentiation of mesenchymal stem cells by the PI3K/β-catenin pathway. J Orthop Res. 33:957–964. 2015. View Article : Google Scholar : PubMed/NCBI

38 

Yuan Y, Du W, Wang Y, Xu C, Wang J, Zhang Y, Wang H, Ju J, Zhao L, Wang Z, et al: Suppression of AKT expression by miR-153 produced anti-tumor activity in lung cancer. Int J Cancer. 136:1333–1340. 2015. View Article : Google Scholar : PubMed/NCBI

39 

Xu H, Abuhatzira L, Carmona GN, Vadrevu S, Satin LS and Notkins AL: The Ia-2β intronic miRNA, miR-153, is a negative regulator of insulin and dopamine secretion through its effect on the Cacna1c gene in mice. Diabetologia. 58:2298–2306. 2015. View Article : Google Scholar : PubMed/NCBI

40 

Hua HW, Jiang F, Huang Q, Liao Z and Ding G: MicroRNA-153 promotes Wnt/β-catenin activation in hepatocellular carcinoma through suppression of WWOX. Oncotarget. 6:3840–3847. 2015. View Article : Google Scholar : PubMed/NCBI

41 

Liang W, Lin M, Li X, Li C, Gao B, Gan H, Yang Z, Lin X, Liao L and Yang M: Icariin promotes bone formation via the BMP-2/Smad4 signal transduction pathway in the hFOB 1.19 human osteoblastic cell line. Int J Mol Med. 30:889–895. 2012. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Huang Z, Cheng C, Wang J, Liu X, Wei H, Han Y, Yang S and Wang X: Icariin regulates the osteoblast differentiation and cell proliferation of MC3T3‑E1 cells through microRNA‑153 by targeting Runt‑related transcription factor 2. Exp Ther Med 15: 5159-5166, 2018.
APA
Huang, Z., Cheng, C., Wang, J., Liu, X., Wei, H., Han, Y. ... Wang, X. (2018). Icariin regulates the osteoblast differentiation and cell proliferation of MC3T3‑E1 cells through microRNA‑153 by targeting Runt‑related transcription factor 2. Experimental and Therapeutic Medicine, 15, 5159-5166. https://doi.org/10.3892/etm.2018.6127
MLA
Huang, Z., Cheng, C., Wang, J., Liu, X., Wei, H., Han, Y., Yang, S., Wang, X."Icariin regulates the osteoblast differentiation and cell proliferation of MC3T3‑E1 cells through microRNA‑153 by targeting Runt‑related transcription factor 2". Experimental and Therapeutic Medicine 15.6 (2018): 5159-5166.
Chicago
Huang, Z., Cheng, C., Wang, J., Liu, X., Wei, H., Han, Y., Yang, S., Wang, X."Icariin regulates the osteoblast differentiation and cell proliferation of MC3T3‑E1 cells through microRNA‑153 by targeting Runt‑related transcription factor 2". Experimental and Therapeutic Medicine 15, no. 6 (2018): 5159-5166. https://doi.org/10.3892/etm.2018.6127
Copy and paste a formatted citation
x
Spandidos Publications style
Huang Z, Cheng C, Wang J, Liu X, Wei H, Han Y, Yang S and Wang X: Icariin regulates the osteoblast differentiation and cell proliferation of MC3T3‑E1 cells through microRNA‑153 by targeting Runt‑related transcription factor 2. Exp Ther Med 15: 5159-5166, 2018.
APA
Huang, Z., Cheng, C., Wang, J., Liu, X., Wei, H., Han, Y. ... Wang, X. (2018). Icariin regulates the osteoblast differentiation and cell proliferation of MC3T3‑E1 cells through microRNA‑153 by targeting Runt‑related transcription factor 2. Experimental and Therapeutic Medicine, 15, 5159-5166. https://doi.org/10.3892/etm.2018.6127
MLA
Huang, Z., Cheng, C., Wang, J., Liu, X., Wei, H., Han, Y., Yang, S., Wang, X."Icariin regulates the osteoblast differentiation and cell proliferation of MC3T3‑E1 cells through microRNA‑153 by targeting Runt‑related transcription factor 2". Experimental and Therapeutic Medicine 15.6 (2018): 5159-5166.
Chicago
Huang, Z., Cheng, C., Wang, J., Liu, X., Wei, H., Han, Y., Yang, S., Wang, X."Icariin regulates the osteoblast differentiation and cell proliferation of MC3T3‑E1 cells through microRNA‑153 by targeting Runt‑related transcription factor 2". Experimental and Therapeutic Medicine 15, no. 6 (2018): 5159-5166. https://doi.org/10.3892/etm.2018.6127
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team