Open Access

A cynomolgus monkey model of carotid atherosclerosis induced by puncturing and scratching of the carotid artery combined with a high‑fat diet

  • Authors:
    • Lei Zhang
    • Yan Zeng
    • Ji Qi
    • Yanxiao Xu
    • Shaoqun Zhang
    • Xin Zhou
    • Ruiyue Ping
    • Shijie Fu
  • View Affiliations

  • Published online on: May 10, 2018     https://doi.org/10.3892/etm.2018.6143
  • Pages: 113-120
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cardio‑cerebrovascular disease is one of the three major causes of mortality in humans and constitutes a major socioeconomic burden. Carotid atherosclerosis (CAS) is a very common lesion of the arterial walls, which leads to narrowing of the arteries, in some cases occluding them entirely, increasing the risk of cardiovascular events. The aim of the present study was to evaluate a cynomolgus monkey model of carotid atherosclerosis (CAS) induced by puncturing and scratching combined with a high‑fat diet. A total of 12 cynomolgus monkeys were randomly divided into four groups: A, puncturing and scratching carotid artery intimas + high‑fat diet (n=3); B, puncturing and scratching carotid artery intimas + regular diet (n=3); C, high‑fat diet only (n=3); and D, regular diet only (n=3). Blood was harvested at weeks 4, 6 and 8 and plasma lipid levels were assessed. At week 8, monkeys were sacrificed and carotid arteries were harvested for hematoxylin and eosin (H&E) staining to observe pathological changes. The results revealed that a high‑fat diet led to increased plasma lipid levels and accelerated plaque formation. Carotid color Doppler ultrasonography was performed and, along with H&E staining, revealed plaque formation in group A. In summary, the results of the present study suggest that a cynomolgus monkey model of CAS model may be successfully constructed by puncturing and scratching of the carotid artery intimas in combination with a high‑fat diet.
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July-2018
Volume 16 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zhang L, Zeng Y, Qi J, Xu Y, Zhang S, Zhou X, Ping R and Fu S: A cynomolgus monkey model of carotid atherosclerosis induced by puncturing and scratching of the carotid artery combined with a high‑fat diet. Exp Ther Med 16: 113-120, 2018
APA
Zhang, L., Zeng, Y., Qi, J., Xu, Y., Zhang, S., Zhou, X. ... Fu, S. (2018). A cynomolgus monkey model of carotid atherosclerosis induced by puncturing and scratching of the carotid artery combined with a high‑fat diet. Experimental and Therapeutic Medicine, 16, 113-120. https://doi.org/10.3892/etm.2018.6143
MLA
Zhang, L., Zeng, Y., Qi, J., Xu, Y., Zhang, S., Zhou, X., Ping, R., Fu, S."A cynomolgus monkey model of carotid atherosclerosis induced by puncturing and scratching of the carotid artery combined with a high‑fat diet". Experimental and Therapeutic Medicine 16.1 (2018): 113-120.
Chicago
Zhang, L., Zeng, Y., Qi, J., Xu, Y., Zhang, S., Zhou, X., Ping, R., Fu, S."A cynomolgus monkey model of carotid atherosclerosis induced by puncturing and scratching of the carotid artery combined with a high‑fat diet". Experimental and Therapeutic Medicine 16, no. 1 (2018): 113-120. https://doi.org/10.3892/etm.2018.6143