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Experimental research on the effect of microRNA‑21 inhibitor on a rat model of intervertebral disc degeneration

  • Authors:
    • Xiaoming Sheng
    • Qingsong Guo
    • Junbo Yu
    • Youjia Xu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, The Affiliated Second Hospital of Soochow University, Suzhou, Jiangsu 215000, P.R. China, Department of General Surgery, The First Affiliated Hospital of Nantong University, Nantong, Jiangsu 225001, P.R. China, Department of Emergency, The First Affiliated Hospital of Nantong University, Nantong, Jiangsu 225001, P.R. China
    Copyright: © Sheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 67-72
    |
    Published online on: May 11, 2018
       https://doi.org/10.3892/etm.2018.6156
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Abstract

Intervertebral disc degeneration is associated with angiogenesis and is the primary cause of disc‑associated disease. Several studies have indicated the importance of microRNA (miR)‑21 in angiogenesis. Thus, the present study aimed to validate the role and underlying mechanisms of miR‑21 in a rat model of intervertebral disc degeneration. A total of 60 specific‑pathogen‑free Sprague‑Dawley rats were used for in vivo experiments. A rat model of intervertebral disc degeneration was established and miR‑21 inhibitor (antagomiR‑21) was administered. The vertebral pulp and annulus fibrosus were isolated for immunohistochemical analysis of hypoxia inducible factor (HIF)‑1α and vascular endothelial growth factor (VEGF) expression. Lumbar spine proteoglycan content was detected with the phloroglucinol method. Disc cell apoptosis was detected with terminal deoxynucleotidyl‑transferase‑mediated dUTP nick end labeling staining. It was revealed that antagomiR‑21 treatment decreased the expression of HIF‑1α and VEGF in the vertebral pulp and annulus fibrosus. Furthermore, antagomiR‑21 treatment increased proteoglycan content and inhibited cell apoptosis in lumbar spines from model rats with intervertebral disc degeneration. In conclusion, antagomiR‑21 treatment exerted a protective role in a rat model of intervertebral disc degeneration, which may provide the basis for a potential therapeutic approach in the treatment of disc‑associated diseases.
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Copy and paste a formatted citation
Spandidos Publications style
Sheng X, Guo Q, Yu J and Xu Y: Experimental research on the effect of microRNA‑21 inhibitor on a rat model of intervertebral disc degeneration. Exp Ther Med 16: 67-72, 2018.
APA
Sheng, X., Guo, Q., Yu, J., & Xu, Y. (2018). Experimental research on the effect of microRNA‑21 inhibitor on a rat model of intervertebral disc degeneration. Experimental and Therapeutic Medicine, 16, 67-72. https://doi.org/10.3892/etm.2018.6156
MLA
Sheng, X., Guo, Q., Yu, J., Xu, Y."Experimental research on the effect of microRNA‑21 inhibitor on a rat model of intervertebral disc degeneration". Experimental and Therapeutic Medicine 16.1 (2018): 67-72.
Chicago
Sheng, X., Guo, Q., Yu, J., Xu, Y."Experimental research on the effect of microRNA‑21 inhibitor on a rat model of intervertebral disc degeneration". Experimental and Therapeutic Medicine 16, no. 1 (2018): 67-72. https://doi.org/10.3892/etm.2018.6156
Copy and paste a formatted citation
x
Spandidos Publications style
Sheng X, Guo Q, Yu J and Xu Y: Experimental research on the effect of microRNA‑21 inhibitor on a rat model of intervertebral disc degeneration. Exp Ther Med 16: 67-72, 2018.
APA
Sheng, X., Guo, Q., Yu, J., & Xu, Y. (2018). Experimental research on the effect of microRNA‑21 inhibitor on a rat model of intervertebral disc degeneration. Experimental and Therapeutic Medicine, 16, 67-72. https://doi.org/10.3892/etm.2018.6156
MLA
Sheng, X., Guo, Q., Yu, J., Xu, Y."Experimental research on the effect of microRNA‑21 inhibitor on a rat model of intervertebral disc degeneration". Experimental and Therapeutic Medicine 16.1 (2018): 67-72.
Chicago
Sheng, X., Guo, Q., Yu, J., Xu, Y."Experimental research on the effect of microRNA‑21 inhibitor on a rat model of intervertebral disc degeneration". Experimental and Therapeutic Medicine 16, no. 1 (2018): 67-72. https://doi.org/10.3892/etm.2018.6156
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