Open Access

Ellagic acid induces HeLa cell apoptosis via regulating signal transducer and activator of transcription 3 signaling

  • Authors:
    • Lian‑Wei Li
    • Chao Na
    • Song‑Yu Tian
    • Jie Chen
    • Rong Ma
    • Ying Gao
    • Ge Lou
  • View Affiliations

  • Published online on: May 17, 2018     https://doi.org/10.3892/etm.2018.6182
  • Pages: 29-36
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Ellagic acid has been reported to possess various activities, including anti-inflammatory, anti-oxidative, antiviral and anticancer abilities. However, the effect and underlying molecular mechanism of ellagic acid on cervical carcinoma remain unclear. Therefore, the present study aimed to investigate the effects of ellagic acid on human cervical carcinoma cells and the molecular mechanism involved. The present study assessed the survival of HeLa cells cultured in vitro using an MTT assay. Apoptosis rate and cell cycle of HaLa cells were measured using an Annexin V‑Fluorescein isothiocyanate/propidium iodide Apoptosis Detection and Cell Cycle Analysis kits, respectively, following treatment with varying concentrations of ellagic acid. Further effects of ellagic acid on HeLa cells was assessed using flow cytometry and western blotting. Ellagic acid treatment significantly inhibited cell proliferation of the human cervical carcinoma HeLa, SiHa and C33A cells. In HeLa cells, it was observed that ellagic acid arrested the cell cycle at G1 phase, induced cell apoptosis, suppressed the phosphorylation of Janus kinase 2 and signal transducer and activator of transcription 3 (STAT3), as well as modulated the expression of associated proteins. Collectively, the results of the present study provide evidence that ellagic acid inhibits cervical carcinoma cell proliferation, and induces apoptosis and cell cycle arrest at G1 phase possibly via the regulation of STAT3 signaling.
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July-2018
Volume 16 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Li LW, Na C, Tian SY, Chen J, Ma R, Gao Y and Lou G: Ellagic acid induces HeLa cell apoptosis via regulating signal transducer and activator of transcription 3 signaling. Exp Ther Med 16: 29-36, 2018
APA
Li, L., Na, C., Tian, S., Chen, J., Ma, R., Gao, Y., & Lou, G. (2018). Ellagic acid induces HeLa cell apoptosis via regulating signal transducer and activator of transcription 3 signaling. Experimental and Therapeutic Medicine, 16, 29-36. https://doi.org/10.3892/etm.2018.6182
MLA
Li, L., Na, C., Tian, S., Chen, J., Ma, R., Gao, Y., Lou, G."Ellagic acid induces HeLa cell apoptosis via regulating signal transducer and activator of transcription 3 signaling". Experimental and Therapeutic Medicine 16.1 (2018): 29-36.
Chicago
Li, L., Na, C., Tian, S., Chen, J., Ma, R., Gao, Y., Lou, G."Ellagic acid induces HeLa cell apoptosis via regulating signal transducer and activator of transcription 3 signaling". Experimental and Therapeutic Medicine 16, no. 1 (2018): 29-36. https://doi.org/10.3892/etm.2018.6182