Open Access

High glucose induces epithelial‑mesenchymal transition and results in the migration and invasion of colorectal cancer cells

  • Authors:
    • Jiayan Wu
    • Jiayi Chen
    • Yang Xi
    • Fuyan Wang
    • Hongcun Sha
    • Lin Luo
    • Yabin Zhu
    • Xiaoming Hong
    • Shizhong Bu
  • View Affiliations

  • Published online on: May 18, 2018     https://doi.org/10.3892/etm.2018.6189
  • Pages: 222-230
  • Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Diabetes mellitus (DM) is associated with an increased risk of colorectal cancer (CRC). Hyperglycemia, a chronic abnormality in diabetes, is an independent predictor of cancer‑associated mortality in CRC. However, the underlying biological mechanism of hyperglycemia in CRC cells is largely unknown. In the present study, HCT‑116 and HT‑29 cell proliferation, apoptosis, migration and invasion were assessed. In addition, the expression of epithelial (E)‑cadherin, vimentin and high‑mobility group A protein 2 (HMGA2) were assessed using western blotting. The results demonstrated that high glucose (HG; 30 mmol/l) caused CRC cells to lose their epithelial morphology, with a decrease in E‑cadherin and an increase in vimentin, suggesting epithelial‑mesenchymal transition (EMT). Furthermore, HG significantly enhanced the cell migration and invasion of CRC cells and the expression of HMGA2. Transfection with HMGA2 small interfering RNA reversed the HG‑induced changes to CRC cells. In addition, HG promoted CRC cell proliferation and suppressed apoptosis. The results of the present study suggest that hyperglycemia promotes EMT, proliferation, migration and invasion in CRC cells and may provide novel insights into the link between HG and CRC.
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July-2018
Volume 16 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Wu J, Chen J, Xi Y, Wang F, Sha H, Luo L, Zhu Y, Hong X and Bu S: High glucose induces epithelial‑mesenchymal transition and results in the migration and invasion of colorectal cancer cells. Exp Ther Med 16: 222-230, 2018
APA
Wu, J., Chen, J., Xi, Y., Wang, F., Sha, H., Luo, L. ... Bu, S. (2018). High glucose induces epithelial‑mesenchymal transition and results in the migration and invasion of colorectal cancer cells. Experimental and Therapeutic Medicine, 16, 222-230. https://doi.org/10.3892/etm.2018.6189
MLA
Wu, J., Chen, J., Xi, Y., Wang, F., Sha, H., Luo, L., Zhu, Y., Hong, X., Bu, S."High glucose induces epithelial‑mesenchymal transition and results in the migration and invasion of colorectal cancer cells". Experimental and Therapeutic Medicine 16.1 (2018): 222-230.
Chicago
Wu, J., Chen, J., Xi, Y., Wang, F., Sha, H., Luo, L., Zhu, Y., Hong, X., Bu, S."High glucose induces epithelial‑mesenchymal transition and results in the migration and invasion of colorectal cancer cells". Experimental and Therapeutic Medicine 16, no. 1 (2018): 222-230. https://doi.org/10.3892/etm.2018.6189