Ellagic acid promotes A549 cell apoptosis via regulating the phosphoinositide 3‑kinase/protein kinase B pathway

  • Authors:
    • Qiong Liu
    • Xiaobing Liang
    • Chengwei Niu
    • Xuelan Wang
  • View Affiliations

  • Published online on: May 18, 2018     https://doi.org/10.3892/etm.2018.6193
  • Pages: 347-352
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Abstract

The present study aimed to evaluate the anti‑cancer effect of ellagic acid in human non‑small cell lung cancer (NSCLC) A549 cells and to reveal the potential underlying mechanism. The effects of ellagic acid on the cell proliferation of A549 cells were determined by MTT assay. Cell cycle and apoptosis were measured with flow cytometry and Annexin V‑propidium iodide staining. Western blotting was used to measure the expression levels of the phosphatidylinositol 3‑kinase (PI3K)/protein kinas B (Akt) signaling pathway and apoptosis‑associated proteins. It was demonstrated that ellagic acid exerted an inhibitory effect in the proliferation of human NSCLC A549 cells. Flow cytometry demonstrated that G1 phase retention and apoptosis rates were significantly increased after treatment with ellagic acid. Further investigation revealed that ellagic acid treatment diminished the phosphorylation of PI3K and Akt and regulated the expression of apoptosis‑associated proteins in A549 cells. In conclusion, the present results indicated that ellagic acid suppresses cell proliferation, arrests cell cycle and induces apoptosis in human NSCLC A549 cells by inhibiting the PI3K/Akt signaling pathway.
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July-2018
Volume 16 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Liu Q, Liang X, Niu C and Wang X: Ellagic acid promotes A549 cell apoptosis via regulating the phosphoinositide 3‑kinase/protein kinase B pathway. Exp Ther Med 16: 347-352, 2018
APA
Liu, Q., Liang, X., Niu, C., & Wang, X. (2018). Ellagic acid promotes A549 cell apoptosis via regulating the phosphoinositide 3‑kinase/protein kinase B pathway. Experimental and Therapeutic Medicine, 16, 347-352. https://doi.org/10.3892/etm.2018.6193
MLA
Liu, Q., Liang, X., Niu, C., Wang, X."Ellagic acid promotes A549 cell apoptosis via regulating the phosphoinositide 3‑kinase/protein kinase B pathway". Experimental and Therapeutic Medicine 16.1 (2018): 347-352.
Chicago
Liu, Q., Liang, X., Niu, C., Wang, X."Ellagic acid promotes A549 cell apoptosis via regulating the phosphoinositide 3‑kinase/protein kinase B pathway". Experimental and Therapeutic Medicine 16, no. 1 (2018): 347-352. https://doi.org/10.3892/etm.2018.6193