Annexin A2 and FTH1 are potential biomarkers for lupus nephritis

Zhou,Yanni; Xiao,Liangxiang; Tang,Shuifu

doi:10.3892/etm.2018.6686

Annexin A2 and FTH1 are potential biomarkers for lupus nephritis

Authors:
- Yanni Zhou
- Liangxiang Xiao
- Shuifu Tang
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Affiliations: Division of Nephrology, Xiamen Hospital of Traditional Chinese Medicine, Fujian University of Traditional Chinese Medicine, Xiamen, Fujian 361000, P.R. China, Division of Nephrology, Zhongshan Hospital Affiliated to Xiamen University, Xiamen, Fujian 361004, P.R. China, Division of Nephrology, First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou, Guangdong 510405, P.R. China
Published online on: September 4, 2018 https://doi.org/10.3892/etm.2018.6686
Pages: 3766-3776
Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Lupus nephritis (LN) occurs in ~50% of patients with systemic lupus erythematosus and is a major cause of morbidity and mortality of the affected individuals. Therefore, identification of novel and predictive biomarkers for the early diagnosis and progression of LN is required. The present study included 10 patients with LN whose diagnoses were confirmed by renal biopsy and 5 healthy participants as control subjects. Sera were collected both from patients with LN and healthy controls. Subsequently, mesangial cells were treated with these sera for 24 h. Differential proteins between groups were detected by two‑dimensional difference gel electrophoresis (2D‑DIGE) and matrix‑assisted laser desorption/ionization time of flight mass spectrometry analysis. 2D‑DIGE maps of cellar proteins were obtained for LN and normal control groups. A total of 45 proteins were characterized, and 2 low‑abundance proteins were identified. Compared with the normal human sera group, expression level of Annexin A2 was elevated in patients with LN, while the expression of the ferritin heavy chain (FTH1) decreased in the LN group; the analysis was carried out by DeCyder version 7.0 automatically. The results of the present study suggest that Annexin A2 and FTH1 contributed to the progression of LN and could serve as potential biomarkers for this disease.

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1	Tsokos GC: Systemic lupus erythematosus. N Engl J Med. 365:2110–2121. 2011. View Article : Google Scholar : PubMed/NCBI
2	Pons-Estel GJ, Alarcon GS, Scofield L, Reinlib L and Cooper GS: Understanding the epidemiology and progression of systemic lupus erythematosus. Semin Arthritis Rheum. 39:257–268. 2010. View Article : Google Scholar : PubMed/NCBI
3	Gregersen PK and Olsson LM: Recent advances in the genetics of autoimmune disease. Annu Rev Immunol. 27:363–391. 2009. View Article : Google Scholar : PubMed/NCBI
4	Waldman M and Appel GB: Update on the treatment of lupus nephritis. Kidney Int. 70:1403–1412. 2006. View Article : Google Scholar : PubMed/NCBI
5	Sprangers B, Monahan M and Appel GB: Diagnosis and treatment of lupus nephritis flares-an update. Nat Rev Nephrol. 8:709–717. 2012. View Article : Google Scholar : PubMed/NCBI
6	Kalloo S, Aggarwal N, Mohan P and Radhakrishnan J: Lupus nephritis: Treatment of resistant disease. Clin J Am Soc Nephrol. 8:154–161. 2013. View Article : Google Scholar : PubMed/NCBI
7	Saxena R, Mahajan T and Mohan C: Lupus nephritis: Current update. Arthritis Res Ther. 13:2402011. View Article : Google Scholar : PubMed/NCBI
8	Waldman M and Madaio MP: Pathogenic autoantibodies in lupus nephritis. Lupus. 14:19–24. 2005. View Article : Google Scholar : PubMed/NCBI
9	Bhat P and Radhakrishnan J: B lymphocytes and lupus nephritis: New insights into pathogenesis and targeted therapies. Kidney Int. 73:261–268. 2008. View Article : Google Scholar : PubMed/NCBI
10	Kahlenberg JM, Thacker SG, Berthier CC, Cohen CD, Kretzler M and Kaplan MJ: Inflammasome activation of IL-18 results in endothelial progenitor cell dysfunction in systemic lupus erythematosus. J Immunol. 187:6143–6156. 2011. View Article : Google Scholar : PubMed/NCBI
11	Ciferska H, Horak P, Konttinen YT, Krejci K, Tichy T, Hermanova Z and Zadrazil J: Expression of nucleic acid binding Toll-like receptors in control, lupus and transplanted kidneys-a preliminary pilot study. Lupus. 17:580–585. 2008. View Article : Google Scholar : PubMed/NCBI
12	Zhang Y, Yang J, Jiang S, Fang C, Xiong L, Cheng H and Xia Y: The lupus-derived anti-double-stranded DNA IgG contributes to myofibroblast-like phenotype in mesangial cells. J Clin Immunol. 32:1270–1278. 2012. View Article : Google Scholar : PubMed/NCBI
13	Seret G, Le Meur Y, Renaudineau Y and Youinou P: Mesangial cell-specific antibodies are central to the pathogenesis of lupus nephritis. Clin Dev Immunol. 2012:5796702012. View Article : Google Scholar : PubMed/NCBI
14	Yung S, Tsang RC, Leung JK and Chan TM: Increased mesangial cell hyaluronan expression in lupus nephritis is mediated by anti-DNA antibody-induced IL-1beta. Kidney Int. 69:272–280. 2006. View Article : Google Scholar : PubMed/NCBI
15	Jeruc J, Jurcic V, Vizjak A, Hvala A, Babic N, Kveder R, Praprotnik S and Ferluga D: Tubulo-interstitial involvement in lupus nephritis with emphasis on pathogenesis. Wien Klin Wochenschr. 112:702–706. 2000.PubMed/NCBI
16	Fiore N, Castellano G, Blasi A, Capobianco C, Loverre A, Montinaro V, Netti S, Torres D, Manno C, Grandaliano G, et al: Immaturemyeloid and plasmacytoid dendritic cells infiltrate renal tubulointerstitium in patients with lupus nephritis. Mol Immunol. 45:259–265. 2008. View Article : Google Scholar : PubMed/NCBI
17	Zhang X, Jin M, Wu H, Nadasdy T, Nadasdy G, Harris N, Green-Church K, Nagaraja H, Birmingham DJ, Yu CY, et al: Biomarkers of lupus nephritis determined by serial urine proteomics. Kidney Int. 74:799–807. 2008. View Article : Google Scholar : PubMed/NCBI
18	Mosley K, Tam FW, Edwards RJ, Crozier J, Pusey CD and Lightstone L: Urinary proteomic profiles distinguish between active and inactive lupus nephritis. Rheumatology (Oxford). 45:1497–1504. 2006. View Article : Google Scholar : PubMed/NCBI
19	Perrot M1, Sagliocco F, Mini T, Monribot C, Schneider U, Shevchenko A, Mann M, Jenö P and Boucherie H: Two-dimensional gel protein database of Saccharomyces cerevisiae (update 1999). Electrophoresis. 20:2280–2298. 1999. View Article : Google Scholar : PubMed/NCBI
20	Patton WF: Detection technologies in proteome analysis. J Chromatogr B Analyt Technol Biomed Life Sci. 771:3–31. 2002. View Article : Google Scholar : PubMed/NCBI
21	Mok CC and Lau CS: Pathogenesis of systemic lupus erythematosus. J Clin Pathol. 56:481–490. 2003. View Article : Google Scholar : PubMed/NCBI
22	Weening JJ, D'Agati VD, Schwartz MM, Seshan SV, Alpers CE, Appel GB, Balow JE, Bruijn JA, Cook T, Ferrario F, et al: The classification of glomerulonephritis in systemic lupus erythematosus revisited. J Am Soc Nephrol. 15:241–250. 2004. View Article : Google Scholar : PubMed/NCBI
23	Stainsby D: Guide to Preparation, Use and Quality Assurance of Blood Components. Recommendation No. R (95) 15. J Clin Pathol. 51:2010.
24	Wei XF, Zhou QG, Hou FF, Liu BY and Liang M: Advanced oxidation protein products induce mesangial cell perturbation through PKC-dependent activation of NADPH oxidase. Am J Physiol Renal Physiol. 296:F427–F437. 2009. View Article : Google Scholar : PubMed/NCBI
25	Bairoch A and Apweiler R: The SWISS-PROT protein sequence database and its supplement TrEMBL in 2000. Nucleic Acids Res. 28:45–48. 2000. View Article : Google Scholar : PubMed/NCBI
26	Xiao L, Zhou D, Tan RJ, Fu H, Zhou L, Hou FF and Liu Y: Sustained activation of wnt/β-catenin signaling drives AKI to CKD progression. J Am Soc Nephrol. 27:1727–1740. 2016. View Article : Google Scholar : PubMed/NCBI
27	Tedeschi SK, Bermas B and Costenbader KH: Sexual disparities in the incidence and course of SLE and RA. Clin Immunol. 149:211–218. 2013. View Article : Google Scholar : PubMed/NCBI
28	Liu YP, Hu SW, Wu ZF, Mei LX, Lang P and Lu XH: Proteomic analysis of human serum from diabetic retinopathy. Int J Ophthalmol. 4:616–622. 2011.PubMed/NCBI
29	Siever DA and Erickson HP: Extracellular Annexin II. Int J Biochem Cell Biol. 29:1219–1223. 1997. View Article : Google Scholar : PubMed/NCBI
30	Kim J and Hajjar KA: Annexin II: A plasminogen-plasminogen activator co-receptor. Front Biosci. 7:d341–d348. 2002. View Article : Google Scholar : PubMed/NCBI
31	Vedeler A, Hollas H, Grindheim AK and Raddum AM: Multiple roles of Annexin A2 in post-transcriptional regulation of gene expression. Curr Protein Pept Sci. 13:401–412. 2012. View Article : Google Scholar : PubMed/NCBI
32	Bharadwaj A, Bydoun M, Holloway R and Waisman D: Annexin A2 heterotetramer: Structure and function. Int J Mol Sci. 14:6259–6305. 2013. View Article : Google Scholar : PubMed/NCBI
33	Cheng CW, Rifai A, Ka SM, Shui HA, Lin YF, Lee WH and Chen A: Calcium-binding proteins Annexin A2 and S100A6 are sensors of tubular injury and recovery in acute renal failure. Kidney Int. 68:2694–2703. 2005. View Article : Google Scholar : PubMed/NCBI
34	Ka SM, Rifai A, Chen JH, Cheng CW, Shui HA, Lee HS, Lin YF, Hsu LF and Chen A: Glomerular crescent-related biomarkers in a murine model of chronic graft versus host disease. Nephrol Dial Transplant. 21:288–298. 2006. View Article : Google Scholar : PubMed/NCBI
35	Yung S, Cheung KF, Zhang Q and Chan TM: Anti-dsDNA antibodies bind to mesangial Annexin II in lupus nephritis. J Am Soc Nephrol. 21:1912–1927. 2010. View Article : Google Scholar : PubMed/NCBI
36	Liu NQ, De Marchi T, Timmermans Am, Beekhof R, Trapman-Jansen AM, Foekens R, Look MP, van Deurzen CH, Span PN, Sweep FC, et al: Ferritin heavy chain in triple negative breast cancer: A favorable prognostic marker that relates to a cluster of differentiation 8 positive (CD8+) effector T-cell response. Mol Cell Proteomics. 13:1814–1827. 2014. View Article : Google Scholar : PubMed/NCBI
37	Wang W, Knovich MA, Coffman LG, Torti FM and Torti SV: Serum ferritin: Past, present and future. Biochim Biophys Acta. 1800:760–769. 2010. View Article : Google Scholar : PubMed/NCBI
38	Recalcati S, Invernizzi P, Arosio P and Cairo G: New functions for an iron storage protein: The role of ferritin in immunity and autoimmunity. J Autoimmun. 30:84–89. 2008. View Article : Google Scholar : PubMed/NCBI
39	Zhang KH, Tian HY, Gao X, Lei WW, Hu Y, Wang DM, Pan XC, Yu ML, Xu GJ, Zhao FK and Song JG: Ferritin heavy chain-mediated iron homeostasis and subsequent increased reactive oxygen species production are essential for epithelial-mesenchymal transition. Cancer Res. 69:5340–5348. 2009. View Article : Google Scholar : PubMed/NCBI
40	Zarjou A, Bolisetty S, Joseph R, Traylor A, Apostolov EO, Arosio P, Balla J, Verlander J, Darshan D, Kuhn LC and Agarwal A: Proximal tubule H-ferritin mediates iron trafficking in acute kidney injury. J Clin Invest. 123:4423–4434. 2013. View Article : Google Scholar : PubMed/NCBI