Suppression effect on IFN‑γ of adipose tissue‑derived mesenchymal stem cells isolated from β2‑microglobulin‑deficient mice

Masuda,Junko; Takayama,Eiji; Ichinohe,Tatsuo; Strober,Warren; Mizuno‑Kamiya,Masako; Ikawa,Tomokatsu; Kitani,Atsushi; Kawaki,Harumi; Fuss,Ivan; Kawamoto,Hiroshi; Seno,Akimasa; Vaidyanath,Arun; Umemura,Naoki; Mizutani,Akifumi; Kasai,Tomonari; Honjo,Yasuko; Satoh,Ayano; Murakami,Hiroshi; Katsura,Yoshimoto; Kondoh,Nobuo; Seno,Masaharu

doi:10.3892/etm.2018.6689

Suppression effect on IFN‑γ of adipose tissue‑derived mesenchymal stem cells isolated from β2‑microglobulin‑deficient mice

Authors:
- Junko Masuda
- Eiji Takayama
- Tatsuo Ichinohe
- Warren Strober
- Masako Mizuno‑Kamiya
- Tomokatsu Ikawa
- Atsushi Kitani
- Harumi Kawaki
- Ivan Fuss
- Hiroshi Kawamoto
- Akimasa Seno
- Arun Vaidyanath
- Naoki Umemura
- Akifumi Mizutani
- Tomonari Kasai
- Yasuko Honjo
- Ayano Satoh
- Hiroshi Murakami
- Yoshimoto Katsura
- Nobuo Kondoh
- Masaharu Seno
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Affiliations: Department of Medical Bioengineering, Graduate School of Natural Science and Technology, Okayama University, Okayama 700‑8530, Japan, Department of Oral Biochemistry, Asahi University School of Dentistry, Gifu 501‑0296, Japan, Department of Hematology and Oncology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima 734‑8553, Japan, Laboratory of Host Defenses, Mucosal Immunity Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA, Laboratory for Immune Regeneration, RIKEN Center for Integrative Medical Sciences, Yokohama 230‑0045, Japan, Department of Immunology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606‑8507, Japan
Published online on: September 4, 2018 https://doi.org/10.3892/etm.2018.6689
Pages: 4277-4282

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Abstract

Administration of bone marrow‑derived mesenchymal stem cells (MSCs) is a possible treatment for graft‑versus‑host disease (GVHD) following allogeneic hematopoietic stem cell transplantation and other inflammatory conditions. To address the mechanism of immunosuppression by MSCs, in particular those derived from adipose tissue (AMSCs), AMSCs were isolated from three different mouse strains, and the suppressive capacity of the AMSCs thus obtained to suppress interferon (IFN)‑γ generation in mixed lymphocyte reaction cultures serving as an in vitro model of GVHD were assessed. It was revealed that the AMSCs had a potent capacity to suppress IFN‑γ production regardless of their strain of origin and that such suppression was not associated with production of interleukin‑10. In addition, the results demonstrated that β2‑microglobulin (β2m)‑deficient AMSCs from β2m‑/‑ mice were also potent suppressor cells, verifying the fact that the mechanism underlying the suppression by AMSCs is independent of major histocompatibility complex (MHC) class I expression or MHC compatibility. As AMSCs appear to have immunosuppressive properties, AMSCs may be a useful source of biological suppressor cells for the control of GVHD in humans.

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