Upregulation of miRNA‑140‑5p inhibits inflammatory cytokines in acute lung injury through the MyD88/NF‑κB signaling pathway by targeting TLR4

Yang,Ye; Liu,Dongdong; Xi,Yin; Li,Juan; Liu,Bin; Li,Junjie

doi:10.3892/etm.2018.6692

Upregulation of miRNA‑140‑5p inhibits inflammatory cytokines in acute lung injury through the MyD88/NF‑κB signaling pathway by targeting TLR4

Authors:
- Ye Yang
- Dongdong Liu
- Yin Xi
- Juan Li
- Bin Liu
- Junjie Li
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Affiliations: Department of Medical Oncology, Sichuan Cancer Hospital, Chengdu, Sichuan 610041, P.R. China, Department of Respiratory Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510120, P.R. China, Surgical Center, Sichuan Cancer Hospital, Chengdu, Sichuan 610041, P.R. China
Published online on: September 4, 2018 https://doi.org/10.3892/etm.2018.6692
Pages: 3913-3920
Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study was designed to determine the effect of miR‑140‑5p on acute lung injury (ALI) and the associated inflammation induced. As a result, miR‑140‑5p expression in mice with ALI was suppressed when compared with the normal group. Downregulation of miR‑140‑5p increased the levels of inflammatory factors induced by ALI [including tumor necrosis factor‑α, interleukin (IL)‑1β, IL‑6 and myeloperoxidase] in an in vitro model of human lung A549 cells. Downregulation of miR‑140‑5p also induced the protein expression of Toll‑like receptor 4 (TLR4), myeloid differentiation primary response 88 (MyD88) and nuclear factor (NF)‑κB in an in vitro model. Overexpression of miR‑140‑5p reduced the levels of inflammation in the in vitro model of ALI via the suppression of the TLR4/MyD88/NF‑κB signaling pathway. The inhibition of TLR4 using a TLR4 inhibitor reduced the proinflammation effects of anti‑miR‑140‑5p in the in vitro model of ALI. The NF‑κB inhibitor also inhibited the proinflammation effects of anti‑miR‑140‑5p in the in vitro model of ALI. Overall, the results of the present study indicated that miR‑140‑5p inhibited ALI‑induced inflammation via the TLR4/MyD88/NF‑κB signaling pathway.

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