Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
November-2018 Volume 16 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2018 Volume 16 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Overexpression of miR‑574‑3p suppresses proliferation and induces apoptosis of chronic myeloid leukemia cells via targeting IL6/JAK/STAT3 pathway

  • Authors:
    • Haoying Yang
    • Jun Zhang
    • Jiuping Li
    • Furong Zhao
    • Yao Shen
    • Xuemei Xing
  • View Affiliations / Copyright

    Affiliations: Department of Blood Transfusion, San Er Ling Yi Hospital Affiliated to School of Medicine, Xi'an Jiaotong University, Hanzhong, Shanxi 723000, P.R. China, Department of Clinical Laboratory, San Er Ling Yi Hospital Affiliated to School of Medicine, Xi'an Jiaotong University, Hanzhong, Shanxi 723000, P.R. China
  • Pages: 4296-4302
    |
    Published online on: September 5, 2018
       https://doi.org/10.3892/etm.2018.6700
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The present study aimed to elucidate the potential roles and regulatory mechanism of microRNA (miR)‑574‑3p in the development of chronic myeloid leukemia (CML). The expression of miR‑574‑3p in peripheral blood obtained from patients with CML was examined. Subsequently, miR‑574‑3p was overexpressed and suppressed in CML K562 cells to further investigate the effects of miR‑574‑3p on cell proliferation, and apoptosis. Furthermore, a luciferase reporter assay was performed to investigate whether interleukin‑6 (IL‑6) was a target of miR‑574‑3p. In addition, the regulatory association between miR‑574‑3p and the IL‑6/Janus kinase (JNK)/signal transducer and activator of transcription‑3 (STAT3) signaling pathway was explored. The expression of miR‑574‑3p in the peripheral blood obtained from patients with CML was significantly lower compared with that in healthy controls. Overexpression of miR‑574‑3p significantly inhibited the proliferation and induced the apoptosis of K562 cells, whereas suppression of miR‑574‑3p exhibited opposite effects. In addition, IL‑6 was identified to be a direct target of miR‑574‑3p. Overexpression of IL‑6 significantly promoted the proliferation and inhibited the apoptosis of K562 cells. Furthermore, overexpression of miR‑574‑3p inhibited the activation of the JAK/STAT3 signaling pathway, which was rescued by overexpression of IL‑6. The results of the current study indicate that miR‑574‑3p overexpression may serve an important role in inhibiting proliferation and inducing apoptosis of K562 cells via suppression of IL‑6/JAK/STAT3 signaling pathway activation. miR‑574‑3p may serve as a potential therapeutic target for CML.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Aquino SS, Gonçalves RP and Silva LB: The pharmacotherapeutic follow-up of patients with chronic myeloid leukemia (CML) on imatinib mesylate therapy. Rev Bras Hematol Hemoter. 31:137–142. 2009. View Article : Google Scholar

2 

Marzag H: Développement de nouvelles réactions éco-compatibles: Application à la synthèse de molécules bioactives. 2013.

3 

Jabbour E and Kantarjian H: Chronic myeloid leukemia: 2014 update on diagnosis, monitoring, and management. Am J Hematol. 89:547–556. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Holyoake TL, Jiang X, Drummond MW, Eaves AC and Eaves CJ: Elucidating critical mechanisms of deregulated stem cell turnover in the chronic phase of chronic myeloid leukemia. Leukemia. 16:549–558. 2002. View Article : Google Scholar : PubMed/NCBI

5 

Chu Y, Zhu H, Lv L, Zhou Y and Huo J: MiRNAs in oesophageal squamous cancer. Neth J Med. 71:69–75. 2013.PubMed/NCBI

6 

Mo MH, Chen L, Fu Y, Wang W and Fu SW: Cell-free circulating miRNA biomarkers in cancer. J Cancer. 3:432–448. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Reddy KB: MicroRNA (miRNA) in cancer. Cancer Cell Int. 15:382015. View Article : Google Scholar : PubMed/NCBI

8 

Arya D, Sachithanandan SP, Ross C, Palakodeti D, Li S and Krishna S: MiRNA182 regulates percentage of myeloid and erythroid cells in chronic myeloid leukemia. Cell Death Dis. 8:e25472017. View Article : Google Scholar : PubMed/NCBI

9 

Xishan Z, Ziying L, Jing D and Gang L: MicroRNA-320a acts as a tumor suppressor by targeting BCR/ABL oncogene in chronic myeloid leukemia. Sci Rep. 5:124602015. View Article : Google Scholar : PubMed/NCBI

10 

Liu Y, Song Y, Ma W, Zheng W and Yin H: Decreased microRNA-30a levels are associated with enhanced ABL1 and BCR-ABL1 expression in chronic myeloid leukemia. Leuk Res. 37:349–356. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Yu Y, Yang L, Zhao M, Zhu S, Kang R, Vernon P, Tang D and Cao L: Targeting microRNA-30a-mediated autophagy enhances imatinib activity against human chronic myeloid leukemia cells. Leukemia. 26:1752–1760. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Li Y, Zhao L, Li N, Miao Y, Zhou H and Jia L: miR-9 regulates the multidrug resistance of chronic myelogenous leukemia by targeting ABCB1. Oncol Rep. 37:2193–2200. 2017. View Article : Google Scholar : PubMed/NCBI

13 

Tatarano S, Chiyomaru T, Kawakami K, Enokida H, Yoshino H, Hidaka H, Nohata N, Yamasaki T, Gotanda T, Tachiwada T, et al: Novel oncogenic function of mesoderm development candidate 1 and its regulation by MiR-574-3p in bladder cancer cell lines. Int J Oncol. 40:951–959. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Krishnan P, Ghosh S, Wang B, Li D, Narasimhan A, Berendt R, Graham K, Mackey JR, Kovalchuk O and Damaraju S: Next generation sequencing profiling identifies miR-574-3p and miR-660-5p as potential novel prognostic markers for breast cancer. BMC Genomics. 16:7352015. View Article : Google Scholar : PubMed/NCBI

15 

Ujihira T, Ikeda K, Suzuki T, Yamaga R, Sato W, Horie-Inoue K, Shigekawa T, Osaki A, Saeki T, Okamoto K, et al: MicroRNA-574-3p, identified by microRNA library-based functional screening, modulates tamoxifen response in breast cancer. Sci Rep. 5:76412015. View Article : Google Scholar : PubMed/NCBI

16 

Su Y, Ni Z, Wang G, Cui J, Wei C, Wang J, Yang Q, Xu Y and Li F: Aberrant expression of microRNAs in gastric cancer and biological significance of miR-574-3p. Int Immunopharmacol. 13:468–475. 2012. View Article : Google Scholar : PubMed/NCBI

17 

Ma L, Zhu Z, Jiang L, Sun X, Lu X, Zhou M, Qian S and Jianyong L: Matrine suppresses cell growth of human chronic myeloid leukemia cells via its inhibition of the interleukin-6/Janus activated kinase/signal transducer and activator of transcription 3 signaling cohort. Leuk Lymphoma. 56:2923–2930. 2015. View Article : Google Scholar : PubMed/NCBI

18 

Xu H, Liu X, Zhou J, Chen X and Zhao J: miR-574-3p acts as a tumor promoter in osteosarcoma by targeting SMAD4 signaling pathway. Oncol Lett. 12:5247–5253. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Okumura T, Kojima H, Miwa T, Sekine S, Hashimoto I, Hojo S, Nagata T and Shimada Y: The expression of microRNA 574-3p as a predictor of postoperative outcome in patients with esophageal squamous cell carcinoma. World J Surg Oncol. 14:2282016. View Article : Google Scholar : PubMed/NCBI

20 

Yamasaki T, Kim EJ, Cerutti H and Ohama T: Argonaute3 is a key player in miRNA-mediated target cleavage and translational repression in Chlamydomonas. Plant J. 85:258–268. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Hong DS, Angelo LS and Kurzrock R: Interleukin-6 and its receptor in cancer: Implications for translational therapeutics. Cancer. 110:1911–1928. 2007. View Article : Google Scholar : PubMed/NCBI

22 

Maeda K, Baba Y, Nagai Y, Miyazaki K, Malykhin A, Nakamura K, Kincade PW, Sakaguchi N and Coggeshall KM: IL-6 blocks a discrete early step in lymphopoiesis. Blood. 106:879–885. 2005. View Article : Google Scholar : PubMed/NCBI

23 

Reynaud D, Pietras E, Barry-Holson K, Mir A, Binnewies M, Jeanne M, Sala-Torra O, Radich JP and Passegué E: IL-6 controls leukemic multipotent progenitor cell fate and contributes to chronic myelogenous leukemia development. Cancer Cell. 20:661–673. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Ciarcia R, Vitiello MT, Galdiero M, Pacilio C, Iovane V, d'Angelo D, Pagnini D, Caparrotti G, Conti D, Tomei V, et al: Imatinib treatment inhibit IL-6, IL-8, NF-KB and AP-1 production and modulate intracellular calcium in CML patients. J Cell Physiol. 227:2798–2803. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Kumar H, Tichkule S, Raj U, Gupta S, Srivastava S and Varadwaj PK: Effect of STAT3 inhibitor in chronic myeloid leukemia associated signaling pathway: A mathematical modeling, simulation and systems biology study. 3 Biotech. 6:402016. View Article : Google Scholar : PubMed/NCBI

26 

Baśkiewicz-Masiuk M and Machaliński B: The role of the STAT5 proteins in the proliferation and apoptosis of the CML and AML cells. Eur J Haematol. 72:420–429. 2004. View Article : Google Scholar : PubMed/NCBI

27 

Jung JH, Kwon TR, Jeong SJ, Kim EO, Sohn EJ, Yun M and Kim SH: Apoptosis induced by tanshinone IIA and cryptotanshinone is mediated by distinct JAK/STAT3/5 and SHP1/2 signaling in chronic myeloid leukemia K562 cells. Evid Based Complement Alternat Med. 2013:8056392013. View Article : Google Scholar : PubMed/NCBI

28 

Kaymaz BT, Cetintaş VB, Aktan C and Kosova B: MicroRNA-520a-5p displays a therapeutic effect upon chronic myelogenous leukemia cells by targeting STAT3 and enhances the anticarcinogenic role of capsaicin. Tumor Biol. 35:8733–8742. 2014. View Article : Google Scholar

29 

Selvi N, Kaymaz BT, Gündüz C, Aktan Ç, Kiper HD, Şahin F, Cömert M, Selvi AF, Kosova B and Saydam G: Bortezomib induces apoptosis by interacting with JAK/STAT pathway in K562 leukemic cells. Tumor Biol. 35:7861–7870. 2014. View Article : Google Scholar

30 

Cai H, Qin X and Yang C: Dehydrocostus lactone suppresses proliferation of human chronic myeloid leukemia cells through Bcr/Abl-JAK/STAT signaling pathways. J Cell Biochem. 118:3381–3390. 2017. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Yang H, Zhang J, Li J, Zhao F, Shen Y and Xing X: Overexpression of miR‑574‑3p suppresses proliferation and induces apoptosis of chronic myeloid leukemia cells via targeting IL6/JAK/STAT3 pathway. Exp Ther Med 16: 4296-4302, 2018.
APA
Yang, H., Zhang, J., Li, J., Zhao, F., Shen, Y., & Xing, X. (2018). Overexpression of miR‑574‑3p suppresses proliferation and induces apoptosis of chronic myeloid leukemia cells via targeting IL6/JAK/STAT3 pathway. Experimental and Therapeutic Medicine, 16, 4296-4302. https://doi.org/10.3892/etm.2018.6700
MLA
Yang, H., Zhang, J., Li, J., Zhao, F., Shen, Y., Xing, X."Overexpression of miR‑574‑3p suppresses proliferation and induces apoptosis of chronic myeloid leukemia cells via targeting IL6/JAK/STAT3 pathway". Experimental and Therapeutic Medicine 16.5 (2018): 4296-4302.
Chicago
Yang, H., Zhang, J., Li, J., Zhao, F., Shen, Y., Xing, X."Overexpression of miR‑574‑3p suppresses proliferation and induces apoptosis of chronic myeloid leukemia cells via targeting IL6/JAK/STAT3 pathway". Experimental and Therapeutic Medicine 16, no. 5 (2018): 4296-4302. https://doi.org/10.3892/etm.2018.6700
Copy and paste a formatted citation
x
Spandidos Publications style
Yang H, Zhang J, Li J, Zhao F, Shen Y and Xing X: Overexpression of miR‑574‑3p suppresses proliferation and induces apoptosis of chronic myeloid leukemia cells via targeting IL6/JAK/STAT3 pathway. Exp Ther Med 16: 4296-4302, 2018.
APA
Yang, H., Zhang, J., Li, J., Zhao, F., Shen, Y., & Xing, X. (2018). Overexpression of miR‑574‑3p suppresses proliferation and induces apoptosis of chronic myeloid leukemia cells via targeting IL6/JAK/STAT3 pathway. Experimental and Therapeutic Medicine, 16, 4296-4302. https://doi.org/10.3892/etm.2018.6700
MLA
Yang, H., Zhang, J., Li, J., Zhao, F., Shen, Y., Xing, X."Overexpression of miR‑574‑3p suppresses proliferation and induces apoptosis of chronic myeloid leukemia cells via targeting IL6/JAK/STAT3 pathway". Experimental and Therapeutic Medicine 16.5 (2018): 4296-4302.
Chicago
Yang, H., Zhang, J., Li, J., Zhao, F., Shen, Y., Xing, X."Overexpression of miR‑574‑3p suppresses proliferation and induces apoptosis of chronic myeloid leukemia cells via targeting IL6/JAK/STAT3 pathway". Experimental and Therapeutic Medicine 16, no. 5 (2018): 4296-4302. https://doi.org/10.3892/etm.2018.6700
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team