An improved surgical procedure to establish a gastroesophageal reflux model with a high incidence of Barrett's esophagus in rats

Wen,Hui; Liu,Tao; Liu,Hua; Teng,Jing‑Hua; Li,Sheng‑Bao

doi:10.3892/etm.2018.6712

An improved surgical procedure to establish a gastroesophageal reflux model with a high incidence of Barrett's esophagus in rats

Authors:
- Hui Wen
- Tao Liu
- Hua Liu
- Jing‑Hua Teng
- Sheng‑Bao Li
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Affiliations: Department of Gastroenterology, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442002, P.R. China, Department of Thoracic Surgery, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442002, P.R. China
Published online on: September 10, 2018 https://doi.org/10.3892/etm.2018.6712
Pages: 3863-3868
Copyright : © Wen et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].

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Abstract

Barrett's esophagus (BE) is a complication of gastroesophageal reflux disease and is a precursor lesion of esophageal adenocarcinoma. In existing BE models, the incidence of BE is typically low and induction is usually time consuming. In the present study, a gastroesophageal reflux model with a high incidence of BE in rats. Rats were divided into a model group and a sham operation group, and anesthetized with an inhalation anesthesia machine. Stomach‑jejunal anastomosis (SJA) and esophagus‑jejunal anastomosis (EJA) were simultaneously performed in the model group. The distance between the Treitz ligament and the gastro‑jejunal anastomosis was shortened to 3 cm. The distance between the SJA and the EJA was prolonged to 1‑1.5 cm. However, 15/40 rats in the model group succumbed to post‑surgical complications (mortality rate was 37.5%). The weight of surviving rats in the model group was significantly lower compared with the sham group rats post‑surgery. Erosions and ulcers were common of the surviving rats in the model group, with an incidence of 80% (20/25). Squamous cell dysplasia was identified in 40% (10/25) of rats in model group. The modified model was well established within 16 weeks. Notably, the modified surgical procedure used enhanced the incidence of BE in rats from 47% in the EJGJ model (as establish by Zhang) to 100%. To conclude, this model can be used as a reliable animal model for basic research on BE.

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