Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
November-2018 Volume 16 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2018 Volume 16 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

IMB‑6G induces endoplasmic reticulum stress‑mediated apoptosis in human nasopharyngeal carcinoma cells

  • Authors:
    • Yeting Pan
    • Yanni Zhang
    • Liang Gong
    • Jianding Zou
    • Boxia Hu
    • Sicong Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Otorhinolaryngology, Affiliated Cixi Hospital of Wenzhou Medical College, Cixi, Zhejiang 315300, P.R. China
  • Pages: 4187-4192
    |
    Published online on: September 11, 2018
       https://doi.org/10.3892/etm.2018.6724
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

IMB‑6G is a novel N‑substituted sophoridine acid that has been reported to have anticancer effects. The purpose of the present study was to investigate the effect and underlying mechanism of IMB‑6G on human nasopharyngeal carcinoma (NPC) cells. The NPC cell line C666‑1 was used in the present study and treated with different concentrations of IMB‑6G (0, 1, 2 and 5 µM) for 24 h. Subsequently, cell viability was determined using the Cell Counting kit‑8 assay and cell apoptosis was analyzed by performing flow cytometry. The expression levels of genes and proteins in the current study were determined using reverse transcription‑quantitative polymerase chain reaction and western blot analysis, respectively. Results indicated that IMB‑6G dose‑dependently inhibited C666‑1 cell viability and induced apoptosis. It was also revealed that IMB‑6G induced apoptosis via inducing endoplasmic reticulum (ER) stress activation. Notably, IMB‑6G administration enhanced the expression levels of Binding immunoglobulin protein and CCAAT‑enhancer‑binding protein homologous protein in C666‑1 cells. Further analysis suggested that IMB‑6G treatment activated inositol‑requiring enzyme 1α (IRE1α) and PKR‑like ER kinase (PERK) signaling pathways in C666‑1 cells. In addition, silencing of IRE1α and PERK significantly reversed IMB‑6G‑induced cell growth inhibition and apoptosis. In conclusion, the present findings indicated that IMB‑6G induced ER stress‑mediated apoptosis through activating IRE1α and PERK signaling pathways. The present study suggests that IMB‑6G may be a promising agent for NPC treatment.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Chang ET and Adami HO: The enigmatic epidemiology of nasopharyngeal carcinoma. Cancer Epidemiol Biomark Prev. 15:1765–1777. 2006. View Article : Google Scholar

2 

Cao SM, Simons MJ and Qian CN: The prevalence and prevention of nasopharyngeal carcinoma in China. Chin J Cancer. 30:114–119. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Lo KW and Huang DP: Genetic and epigenetic changes in nasopharyngeal carcinoma. Semin Cancer Biol. 12:451–462. 2002. View Article : Google Scholar : PubMed/NCBI

4 

Tao Q and Chan AT: Nasopharyngeal carcinoma: Molecular pathogenesis and therapeutic developments. Expert Rev Mol Med. 9:1–24. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Young LS and Murray PG: Epstein-Barr virus and oncogenesis: From latent genes to tumours. Oncogene. 22:5108–5121. 2003. View Article : Google Scholar : PubMed/NCBI

6 

Katano A, Takahashi W, Yamashita H, Yamamoto K, Ando M, Yoshida M, Saito Y, Abe O and Nakagawa K: Radiotherapy alone and with concurrent chemotherapy for nasopharyngeal carcinoma: A retrospective study. Medicine (Baltimore). 97:e05022018. View Article : Google Scholar : PubMed/NCBI

7 

Chiang AK, Mak NK and Ng WT: Translational research in nasopharyngeal carcinoma. Oral Oncol. 50:345–352. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Wang J, Guo LP, Chen LZ, Zeng YX and Lu SH: Identifcation of cancer stem cell-like side population cells in human nasopharyngeal carcinoma cell line. Cancer Res. 67:3716–3724. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Sze H, Blanchard P, Ng WT, Pignon JP and Lee AW: Chemotherapy for nasopharyngeal carcinoma-current recommendation and controversies. Hematol Oncol Clin North Am. 29:1107–1122. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Wang WX, Sun ZH, Chen HM, Xu BN and Wang FY: Role and mechanism of Sophoridine on proliferation inhibition in human glioma U87MG cell line. Int J Clin Exp Med. 8:464–471. 2015.PubMed/NCBI

11 

Liang L, Wang XY, Zhang XH, Ji B, Yan HC, Deng HZ and Wu XR: Sophoridine exerts an anti-colorectal carcinoma effect through apoptosis induction in vitro and in vivo. Life Sci. 91:1295–1303. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Zhao WC, Song LJ and Deng HZ: Effect of sophoridine on dextran sulfate sodium-induced colitis in C57BL/6 mice. J Asian Nat Prod Res. 12:925–933. 2010. View Article : Google Scholar : PubMed/NCBI

13 

Bi CW, Zhang CX, Li YH, Tang S, Deng HB, Zhao WL, Wang Z, Shao RG and Song DQ: Novel N-substituted sophoridinol derivatives as anticancer agents. Eur J Med Chem. 81:95–105. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Zhang N, Bi C, Liu L, Dou Y, Tang S, Pang W, Deng H and Song D: IMB-6G, a novel N-substituted sophoridinic acid derivative, induces endoplasmic reticulum stress-mediated apoptosis via activation of IRE1α and PERK signaling. Oncotarget. 7:23860–23873. 2016.PubMed/NCBI

15 

Bi C, Zhang C, Li Y, Tang S, Wang S, Shao R, Fu H, Su F and Song D: Synthesis and biological evaluation of sophoridinol derivatives as a novel family of potential anticancer agents. ACS Med Chem Lett. 5:1225–1229. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2011. View Article : Google Scholar

17 

Herold MJ, O'Reilly LA, Lin A, Srivastava R, Doerflinger M, Bouillet P, Strasser A and Puthalakath H: Evidence against upstream regulation of the unfolded protein response (UPR) by pro-apoptotic BIM and PUMA. Cell Death Dis. 5:e13542014. View Article : Google Scholar : PubMed/NCBI

18 

Verfaillie T, Garg AD and Agostinis P: Targeting ER stress induced apoptosis and inflammation in cancer. Cancer Lett. 332:249–264. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Gao W, Chan JY and Wong TS: Curcumin exerts inhibitory effects on undifferentiated nasopharyngeal carcinoma by inhibiting the expression of miR-125a-5p. Clin Sci (Lond). 127:571–579. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Peng H, Chen L, Chen YP, Li WF, Tang LL, Lin AH, Sun Y and Ma J: The current status of clinical trials focusing on nasopharyngeal carcinoma: A comprehensive analysis of clinical trials.gov database. PLoS One. 13:e01967302018. View Article : Google Scholar : PubMed/NCBI

21 

Wang M and Kaufman RJ: The impact of the endoplasmic reticulum protein-folding environment on cancer development. Nat Rev Cancer. 14:581–597. 2014. View Article : Google Scholar : PubMed/NCBI

22 

Walter P and Ron D: The unfolded protein response: From stress pathway to homeostatic regulation. Science. 334:1081–1086. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Ron D and Hubbard SR: How IRE1 reacts to ER stress. Cell. 132:24–26. 2008. View Article : Google Scholar : PubMed/NCBI

24 

Ghosh AP, Klocke BJ, Ballestas ME and Roth KA: CHOP potentially co-operates with FOXO3a in neuronal cells to regulate PUMA and BIM expression in response to ER stress. PLoS One. 7:e395862012. View Article : Google Scholar : PubMed/NCBI

25 

Han J, Back SH, Hur J, Lin YH, Gildersleeve R, Shan J, Yuan CL, Krokowski D, Wang S, Hatzoglou M, et al: ER-stress-induced transcriptional regulation increases protein synthesis leading to cell death. Nat Cell Biol. 15:481–490. 2013. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Pan Y, Zhang Y, Gong L, Zou J, Hu B and Zhang S: IMB‑6G induces endoplasmic reticulum stress‑mediated apoptosis in human nasopharyngeal carcinoma cells. Exp Ther Med 16: 4187-4192, 2018.
APA
Pan, Y., Zhang, Y., Gong, L., Zou, J., Hu, B., & Zhang, S. (2018). IMB‑6G induces endoplasmic reticulum stress‑mediated apoptosis in human nasopharyngeal carcinoma cells. Experimental and Therapeutic Medicine, 16, 4187-4192. https://doi.org/10.3892/etm.2018.6724
MLA
Pan, Y., Zhang, Y., Gong, L., Zou, J., Hu, B., Zhang, S."IMB‑6G induces endoplasmic reticulum stress‑mediated apoptosis in human nasopharyngeal carcinoma cells". Experimental and Therapeutic Medicine 16.5 (2018): 4187-4192.
Chicago
Pan, Y., Zhang, Y., Gong, L., Zou, J., Hu, B., Zhang, S."IMB‑6G induces endoplasmic reticulum stress‑mediated apoptosis in human nasopharyngeal carcinoma cells". Experimental and Therapeutic Medicine 16, no. 5 (2018): 4187-4192. https://doi.org/10.3892/etm.2018.6724
Copy and paste a formatted citation
x
Spandidos Publications style
Pan Y, Zhang Y, Gong L, Zou J, Hu B and Zhang S: IMB‑6G induces endoplasmic reticulum stress‑mediated apoptosis in human nasopharyngeal carcinoma cells. Exp Ther Med 16: 4187-4192, 2018.
APA
Pan, Y., Zhang, Y., Gong, L., Zou, J., Hu, B., & Zhang, S. (2018). IMB‑6G induces endoplasmic reticulum stress‑mediated apoptosis in human nasopharyngeal carcinoma cells. Experimental and Therapeutic Medicine, 16, 4187-4192. https://doi.org/10.3892/etm.2018.6724
MLA
Pan, Y., Zhang, Y., Gong, L., Zou, J., Hu, B., Zhang, S."IMB‑6G induces endoplasmic reticulum stress‑mediated apoptosis in human nasopharyngeal carcinoma cells". Experimental and Therapeutic Medicine 16.5 (2018): 4187-4192.
Chicago
Pan, Y., Zhang, Y., Gong, L., Zou, J., Hu, B., Zhang, S."IMB‑6G induces endoplasmic reticulum stress‑mediated apoptosis in human nasopharyngeal carcinoma cells". Experimental and Therapeutic Medicine 16, no. 5 (2018): 4187-4192. https://doi.org/10.3892/etm.2018.6724
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team