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Enhancement of osteogenesis of rabbit bone marrow derived mesenchymal stem cells by transfection of human BMP‑2 and EGFP recombinant adenovirus via Wnt signaling pathway

  • Authors:
    • Cheng‑Cong Wu
    • Fang Wang
    • Shu Rong
    • Jing Ren
    • Jian‑Shan Wan
    • Li‑Xiang Shi
    • Zhen Wu
    • Tao Liu
    • Qiang Li
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics (I), Qujing No. 1 Hospital, Qujing, Yunnan 655000, P.R. China, Department of Pathology, Qujing Second Hospital, Qujing, Yunnan 655000, P.R. China, Emergency Trauma Surgery, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi 541001, P.R. China
    Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4030-4036
    |
    Published online on: September 13, 2018
       https://doi.org/10.3892/etm.2018.6735
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Abstract

Bone marrow mesenchymal stem cells (BMSCs) are considered the most important seed cells in bone tissue engineering. The present study aimed to investigate the potential of rabbit BMSCs in osteogenesis after the transfection of human BMP‑2 and EGFP recombinant adenovirus. Rabbit BMSCs were isolated and the surface stem cell makers, including CD29, CD44 and CD45 were detected by flow cytometry. The expression of BMP‑2 mRNA and protein in BMSCs were detected by reverse transcription‑quantitative polymerase chain reaction and western blot analysis, respectively. After an induction with osteogenic medium, the alkaline phosphatase (ALK) activity at 7 days, the type I collagen at 14 days, and the calcium nodules at 21 days were performed using an ALK activity kit, immunohistochemical staining and alizarin red S staining, respectively. The expression levels of proteins related to the Wnt signaling pathway were detected by western blot analysis. The positive rates of CD29, CD44 and CD45 were 62.92±1.99, 93.55±0.99 and 0.21±0.12%. The expression of BMP‑2 mRNA and protein was significantly upregulated in Ad‑BMP‑2/EGFP transfected BMSCs. Furthermore, Ad‑BMP‑2/EGFP induced ALP activity, promoted the production of type I collagen and calcium nodule formation in rabbit BMSCs. The levels of β‑catenin, cyclin D1, Runx2 and c‑myc were upregulated in Ad‑hBMP‑2/EGFP transfected BMSCs, while the level of GSK3β was significantly decreased. Results also indicated that the overexpression of BMP‑2 by Ad‑hBMP‑2/EGFP enhanced the osteogenic differentiation ability of cultured rabbit BMSCs via stimulating the Wnt signaling pathway with the accumulation of β‑catenin and suppression of GSK3β. The Ad‑hBMP‑2/EGFP transfected rabbit BMSCs are expected to be a good seed cell in bone tissue engineering.
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1 

Lutolf MP and Hubbell JA: Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering. Nat Biotechnol. 23:47–55. 2005. View Article : Google Scholar : PubMed/NCBI

2 

Nakashima M and Reddi AH: The application of bone morphogenetic proteins to dental tissue engineering. Nat Biotechnol. 21:1025–1032. 2003. View Article : Google Scholar : PubMed/NCBI

3 

Hosseinkhani H, Hong PD and Yu DS: Self-assembled proteins and peptides for regenerative medicine. Chem Rev. 113:4837–4861. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Main RP: Osteocytes and the bone lacunar-canalicular system: Insights into bone biology and skeletal function using bone tissue microstructure. Int J Paleopathol. 18:44–46. 2017. View Article : Google Scholar : PubMed/NCBI

5 

Rao SH, Harini B, Shadamarshan RPK, Balagangadharan K and Selvamurugan N: Natural and synthetic polymers/bioceramics/bioactive compounds-mediated cell signalling in bone tissue engineering. Int J Biol Macromol. 110:88–96. 2018. View Article : Google Scholar : PubMed/NCBI

6 

Daly AC, Freeman FE, Gonzalez-Fernandez T, Critchley SE, Nulty J and Kelly DJ: 3D bioprinting for cartilage and osteochondral tissue engineering. Adv Healthc Mater. 6:2017. View Article : Google Scholar : PubMed/NCBI

7 

Bianco P and Robey PG: Stem cells in tissue engineering. Nature. 414:118–121. 2001. View Article : Google Scholar : PubMed/NCBI

8 

Reddi AH: Role of morphogenetic proteins in skeletal tissue engineering and regeneration. Nat Biotechnol. 16:247–252. 1998. View Article : Google Scholar : PubMed/NCBI

9 

El-Rashidy AA, Roether JA, Harhaus L, Kneser U and Boccaccini AR: Regenerating bone with bioactive glass scaffolds: A review of in vivo studies in bone defect models. Acta Biomater. 62:1–28. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Barcak EA and Beebe MJ: Bone morphogenetic protein: Is there still a role in orthopedic trauma in 2017? Orthop Clin North Am. 48:301–309. 2017. View Article : Google Scholar : PubMed/NCBI

11 

Krishnakumar GS, Roffi A, Reale D, Kon E and Filardo G: Clinical application of bone morphogenetic proteins for bone healing: A systematic review. Int Orthop. 41:1073–1083. 2017. View Article : Google Scholar : PubMed/NCBI

12 

Nguyen V, Meyers CA, Yan N, Agarwal S, Levi B and James AW: BMP-2-induced bone formation and neural inflammation. J Orthop. 14:252–256. 2017. View Article : Google Scholar : PubMed/NCBI

13 

Vijayan V and Gupta S and Gupta S: Bone morphogenetic protein-5, a key molecule that mediates differentiation in MC3T3E1 osteoblast cell line. Biofactors. 43:558–566. 2017. View Article : Google Scholar : PubMed/NCBI

14 

Yoo HS, Chung KH, Lee KJ, Kim DH and An JH: Melanin extract from Gallus gallus domesticus promotes proliferation and differentiation of osteoblastic MG-63 cells via bone morphogenetic protein-2 signaling. Nutr Res Pract. 11:190–197. 2017. View Article : Google Scholar : PubMed/NCBI

15 

Yang D, Anderson PH, Wijenayaka AR, Barratt KR, Triliana R, Stapledon CJM, Zhou H, Findlay DM, Morris HA and Atkins GJ: Both ligand and VDR expression levels critically determine the effect of 1α,25-dihydroxyvitamin-D3 on osteoblast differentiation. J Steroid Biochem Mol Biol. 177:83–90. 2018. View Article : Google Scholar : PubMed/NCBI

16 

Kubota K, Iseki S, Kuroda S, Oida S, Iimura T, Duarte WR, Ohya K, Ishikawa I and Kasugai S: Synergistic effect of fibroblast growth factor-4 in ectopic bone formation induced by bone morphogenetic protein-2. Bone. 31:465–471. 2002. View Article : Google Scholar : PubMed/NCBI

17 

Narayanan KB and Han SS: Icosahedral plant viral nanoparticles-bioinspired synthesis of nanomaterials/nanostructures. Adv Colloid Interface Sci. 248:1–19. 2017. View Article : Google Scholar : PubMed/NCBI

18 

Lai RF, Li ZJ, Zhou ZY, Feng ZQ and Zhao QT: Effect of rhBMP-2 sustained-release nanocapsules on the ectopic osteogenesis process in Sprague-Dawley rats. Asian Pac J Trop Med. 6:884–888. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Betz VM, Betz OB, Rosin T, Keller A, Thirion C, Salomon M, Manthey S, Augat P, Jansson V, Müller PE, et al: The effect of BMP-7 gene activated muscle tissue implants on the repair of large segmental bone defects. Injury. 46:2351–2358. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Cao H, Sun ZB, Zhang L, Qian W, Li CY, Guo XP and Zhang Y: Adenovirus-mediated bone morphogenetic protein-2 promotes osteogenic differentiation in human mesenchymal stem cells in vitro. Exp Ther Med. 14:377–382. 2017. View Article : Google Scholar : PubMed/NCBI

21 

Hu M, Cui F, Liu F, Wang J, Wei X and Li Y: BMP signaling pathways affect differently migration and invasion of esophageal squamous cancer cells. Int J Oncol. 50:193–202. 2017. View Article : Google Scholar : PubMed/NCBI

22 

Park S, Heo HA, Lee KB, Kim HG and Pyo SW: Improved bone regeneration with multiporous PLGA scaffold and BMP-2-transduced human adipose-derived stem cells by cell-permeable peptide. Implant Dent. 26:4–11. 2017. View Article : Google Scholar : PubMed/NCBI

23 

Duan P and Bonewald LF: The role of the wnt/β-catenin signaling pathway in formation and maintenance of bone and teeth. Int J Biochem Cell Biol. 77:23–29. 2016. View Article : Google Scholar : PubMed/NCBI

24 

Kobayashi Y, Uehara S, Udagawa N and Takahashi N: Regulation of bone metabolism by Wnt signals. J Biochem. 159:387–392. 2016. View Article : Google Scholar : PubMed/NCBI

25 

Xu J, Li Z, Hou Y and Fang W: Potential mechanisms underlying the Runx2 induced osteogenesis of bone marrow mesenchymal stem cells. Am J Transl Res. 7:2527–2535. 2015.PubMed/NCBI

26 

Liu JX, Yan ZP, Zhang YY, Wu J, Liu XH and Zeng Y: Hemodynamic shear stress regulates the transcriptional expression of heparan sulfate proteoglycans in human umbilical vein endothelial cell. Cell Mol Biol (Noisy-le-grand). 62:28–34. 2016.PubMed/NCBI

27 

Deng Y, Guo T, Li J, Guo L, Gu P and Fan X: Repair of calvarial bone defect using jarid1a-knockdown bone mesenchymal stem cells in rats. Tissue Eng Part A. 24:711–718. 2018. View Article : Google Scholar : PubMed/NCBI

28 

Lin H, Tang Y, Lozito TP, Oyster N, Kang RB, Fritch MR, Wang B and Tuan RS: Projection stereolithographic fabrication of BMP-2 gene-activated matrix for bone tissue engineering. Sci Rep. 7:113272017. View Article : Google Scholar : PubMed/NCBI

29 

An G, Zhang WB, Ma DK, Lu B, Wei GJ, Guang Y, Ru CH and Wang YS: Influence of VEGF/BMP-2 on the proliferation and osteogenetic differentiation of rat bone mesenchymal stem cells on PLGA/gelatin composite scaffold. Eur Rev Med Pharmacol Sci. 21:2316–2328. 2017.PubMed/NCBI

30 

Wei F, Zhou Y, Wang J, Liu C and Xiao Y: The immunomodulatory role of BMP-2 on macrophages to accelerate osteogenesis. Tissue Eng Part A. 24:584–594. 2018. View Article : Google Scholar : PubMed/NCBI

31 

Ishikawa H, Kitoh H, Sugiura F and Ishiguro N: The effect of recombinant human bone morphogenetic protein-2 on the osteogenic potential of rat mesenchymal stem cells after several passages. Acta Orthop. 78:285–292. 2007. View Article : Google Scholar : PubMed/NCBI

32 

Simmons CA, Alsberg E, Hsiong S, Kim WJ and Mooney DJ: Dual growth factor delivery and controlled scaffold degradation enhance in vivo bone formation by transplanted bone marrow stromal cells. Bone. 35:562–569. 2004. View Article : Google Scholar : PubMed/NCBI

33 

Roostaeian J, Carlsen B, Simhaee D, Jarrahy R, Huang W, Ishida K, Rudkin GH, Yamaguchi DT and Miller TA: Characterization of growth and osteogenic differentiation of rabbit bone marrow stromal cells. J Surg Res. 133:76–83. 2006. View Article : Google Scholar : PubMed/NCBI

34 

Wang A, Ding X, Sheng S and Yao Z: Bone morphogenetic protein receptor in the osteogenic differentiation of rat bone marrow stromal cells. Yonsei Med J. 51:740–745. 2010. View Article : Google Scholar : PubMed/NCBI

35 

Zhu L, Chuanchang D, Wei L, Yilin C and Jiasheng D: Enhanced healing of goat femur-defect using BMP7 gene-modified BMSCs and load-bearing tissue-engineered bone. J Orthop Res. 28:412–418. 2010.PubMed/NCBI

36 

Xu XL, Tang T, Dai K, Zhu Z, Guo XE, Yu C and Lou J: Immune response and effect of adenovirus-mediated human BMP-2 gene transfer on the repair of segmental tibial bone defects in goats. Acta Orthop. 76:637–646. 2005. View Article : Google Scholar : PubMed/NCBI

37 

Li X, Peng J, Wu M, Ye H, Zheng C, Wu G, Xu H, Chen X and Liu X: BMP2 promotes chondrocyte proliferation via the Wnt/β-catenin signaling pathway. Mol Med Rep. 4:621–626. 2011.PubMed/NCBI

38 

Qian C, Zhu C, Yu W, Jiang X, Zhang F and Sun J: Bone morphogenetic protein 2 promotes osteogenesis of bone marrow stromal cells in type 2 diabetic rats via the Wnt signaling pathway. Int J Biochem Cell Biol. 80:143–153. 2016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wu CC, Wang F, Rong S, Ren J, Wan JS, Shi LX, Wu Z, Liu T and Li Q: Enhancement of osteogenesis of rabbit bone marrow derived mesenchymal stem cells by transfection of human BMP‑2 and EGFP recombinant adenovirus via Wnt signaling pathway. Exp Ther Med 16: 4030-4036, 2018.
APA
Wu, C., Wang, F., Rong, S., Ren, J., Wan, J., Shi, L. ... Li, Q. (2018). Enhancement of osteogenesis of rabbit bone marrow derived mesenchymal stem cells by transfection of human BMP‑2 and EGFP recombinant adenovirus via Wnt signaling pathway. Experimental and Therapeutic Medicine, 16, 4030-4036. https://doi.org/10.3892/etm.2018.6735
MLA
Wu, C., Wang, F., Rong, S., Ren, J., Wan, J., Shi, L., Wu, Z., Liu, T., Li, Q."Enhancement of osteogenesis of rabbit bone marrow derived mesenchymal stem cells by transfection of human BMP‑2 and EGFP recombinant adenovirus via Wnt signaling pathway". Experimental and Therapeutic Medicine 16.5 (2018): 4030-4036.
Chicago
Wu, C., Wang, F., Rong, S., Ren, J., Wan, J., Shi, L., Wu, Z., Liu, T., Li, Q."Enhancement of osteogenesis of rabbit bone marrow derived mesenchymal stem cells by transfection of human BMP‑2 and EGFP recombinant adenovirus via Wnt signaling pathway". Experimental and Therapeutic Medicine 16, no. 5 (2018): 4030-4036. https://doi.org/10.3892/etm.2018.6735
Copy and paste a formatted citation
x
Spandidos Publications style
Wu CC, Wang F, Rong S, Ren J, Wan JS, Shi LX, Wu Z, Liu T and Li Q: Enhancement of osteogenesis of rabbit bone marrow derived mesenchymal stem cells by transfection of human BMP‑2 and EGFP recombinant adenovirus via Wnt signaling pathway. Exp Ther Med 16: 4030-4036, 2018.
APA
Wu, C., Wang, F., Rong, S., Ren, J., Wan, J., Shi, L. ... Li, Q. (2018). Enhancement of osteogenesis of rabbit bone marrow derived mesenchymal stem cells by transfection of human BMP‑2 and EGFP recombinant adenovirus via Wnt signaling pathway. Experimental and Therapeutic Medicine, 16, 4030-4036. https://doi.org/10.3892/etm.2018.6735
MLA
Wu, C., Wang, F., Rong, S., Ren, J., Wan, J., Shi, L., Wu, Z., Liu, T., Li, Q."Enhancement of osteogenesis of rabbit bone marrow derived mesenchymal stem cells by transfection of human BMP‑2 and EGFP recombinant adenovirus via Wnt signaling pathway". Experimental and Therapeutic Medicine 16.5 (2018): 4030-4036.
Chicago
Wu, C., Wang, F., Rong, S., Ren, J., Wan, J., Shi, L., Wu, Z., Liu, T., Li, Q."Enhancement of osteogenesis of rabbit bone marrow derived mesenchymal stem cells by transfection of human BMP‑2 and EGFP recombinant adenovirus via Wnt signaling pathway". Experimental and Therapeutic Medicine 16, no. 5 (2018): 4030-4036. https://doi.org/10.3892/etm.2018.6735
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