MicroRNA‑490‑3p regulates cell proliferation and apoptosis in gastric cancer via direct targeting of AKT1 Retraction in /10.3892/etm.2025.13010

Yu,Hong; Sun,Jianying; Jiang,Shaochang; Xu,Ying

doi:10.3892/etm.2018.7042

February-2019 Volume 17 Issue 2

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February-2019 Volume 17 Issue 2

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Article Open Access

MicroRNA‑490‑3p regulates cell proliferation and apoptosis in gastric cancer via direct targeting of AKT1

Retraction in: /10.3892/etm.2025.13010

Authors:
- Hong Yu
- Jianying Sun
- Shaochang Jiang
- Ying Xu
View Affiliations / Copyright

Affiliations: Department of Gastroenterology, Yantai Yuhuangding Hospital, Yantai, Shandong 264000, P.R. China, Department of Internal Medicine, Yantai Yuhuangding Hospital, Yantai, Shandong 264000, P.R. China, Department of Rehabilitation Medicine, Yantai Yuhuangding Hospital, Yantai, Shandong 264000, P.R. China

Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 1330-1336
|
Published online on: December 3, 2018

https://doi.org/10.3892/etm.2018.7042
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Abstract

microRNA (miRNA) expression profiles of gastric cancer (GC) and adjacent healthy gastric mucosa tissue were used to search for differentially expressed miRNAs, identifying downregulated miRNA‑490‑3p in GC. The present study aimed to investigate the cellular function of miRNA‑490‑3p and its underlying mechanism in the occurrence and progression of GC. Reverse transcription‑quantitative polymerase chain reaction was used to measure miRNA‑490‑3p expression levels in GC tissue and adjacent healthy tissue samples. The regulatory effect of miRNA‑490‑3p on the proliferation and apoptosis of GC cells was detected by cell counting kit‑8, colony formation assay and flow cytometry. Bioinformatic methods were used to predict AKT1 as the target of miRNA‑490‑3p and this was verified by a dual‑luciferase reporter assay. Furthermore, western blot analysis was used to measure protein expression of AKT1 in GC cells following overexpression or knockdown of miRNA‑490‑3p. The present study demonstrated that miRNA‑490‑3p expression was downregulated in GC tissue, compared with adjacent healthy tissue. In particular, miRNA‑490‑3p expression levels were significantly decreased in GC tissue samples from patients with advanced cancer (stage III+IV) compared with samples from patients with early‑stage (stage I+II) cancer. Additionally, miRNA‑490‑3p expression levels were significantly decreased in GC tissue samples from patients whose tumor size was >3 cm, compared with those <3 cm. In vitro, downregulation of miRNA‑490‑3p promoted cell proliferation and suppressed apoptosis. In addition, rescue experiments demonstrated that overexpression of AKT1 partially reversed the effect of miRNA‑490‑3p on cell proliferation and apoptosis. The present study demonstrated that miRNA‑490‑3p regulated proliferation and apoptosis in gastric cancer cells via direct targeting of AKT1.

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1	Karimi P, Islami F, Anandasabapathy S, Freedman ND and Kamangar F: Gastric cancer: Descriptive epidemiology, risk factors, screening, and prevention. Cancer Epidemiol Biomarkers Prev. 23:700–713. 2014. View Article : Google Scholar : PubMed/NCBI
2	Hamashima C: Current issues and future perspectives of gastric cancer screening. World J Gastroenterol. 20:13767–13774. 2014. View Article : Google Scholar : PubMed/NCBI
3	Tong XH, Xu B, Zhang YW, Liu YS and Ma CH: Research resources: Comparative microRNA profiles in human corona radiata cells and cumulus oophorus cells detected by next-generation small RNA sequencing. PLoS One. 9:e1067062014. View Article : Google Scholar : PubMed/NCBI
4	Aghanoori MR, Mirzaei B and Tavallaei M: MiRNA molecular profiles in human medical conditions: Connecting lung cancer and lung development phenomena. Asian Pac J Cancer Prev. 15:9557–9565. 2014. View Article : Google Scholar : PubMed/NCBI
5	Hua HB, Yan TT and Sun QM: miRNA polymorphisms and risk of gastric cancer in Asian population. World J Gastroenterol. 20:5700–5707. 2014. View Article : Google Scholar : PubMed/NCBI
6	Wang HB, Jiang ZB and Li M: Research on the typical miRNA and target genes in squamous cell carcinoma and adenocarcinoma of esophagus cancer with DNA microarray. Pathol Oncol Res. 20:245–252. 2014. View Article : Google Scholar : PubMed/NCBI
7	Gailhouste L, Gomez-Santos L and Ochiya T: Potential applications of miRNAs as diagnostic and prognostic markers in liver cancer. Front Biosci (Landmark Ed). 18:199–223. 2013. View Article : Google Scholar : PubMed/NCBI
8	Tölle A, Ratert N and Jung K: miRNA panels as biomarkers for bladder cancer. Biomark Med. 8:733–746. 2014. View Article : Google Scholar : PubMed/NCBI
9	Rajaram K, Harding RL, Bailey T, Patton JG and Hyde DR: Dynamic miRNA expression patterns during retinal regeneration in zebrafish: Reduced dicer or miRNA expression suppresses proliferation of Müller glia-derived neuronal progenitor cells. Dev Dyn. 243:1591–1605. 2014. View Article : Google Scholar : PubMed/NCBI
10	Zhang Y, Ma Y, Xu W, Li W, Min P, Qiu J, Li M, Tang F, Zhang M, Yang D and Zhang J: Association of microRNA-933 variant with the susceptibility to gastric cancer. J BUON. 22:390–395. 2017.PubMed/NCBI
11	Yan H, Wang S, Yu H, Zhu J and Chen C: Molecular pathways and functional analysis of miRNA expression associated with paclitaxel-induced apoptosis in hepatocellular carcinoma cells. Pharmacology. 92:167–174. 2013. View Article : Google Scholar : PubMed/NCBI
12	Tutar Y: miRNA and cancer; computational and experimental approaches. Curr Pharm Biotechnol. 15:4292014. View Article : Google Scholar : PubMed/NCBI
13	Ooms LM, Binge LC, Davies EM, Rahman P, Conway JR, Gurung R, Ferguson DT, Papa A, Fedele CG, Vieusseux JL, et al: The inositol polyphosphate 5-Phosphatase PIPP regulates AKT1-Dependent breast cancer growth and metastasis. Cancer Cell. 28:155–169. 2015. View Article : Google Scholar : PubMed/NCBI
14	Sahlberg SH, Spiegelberg D, Glimelius B, Stenerlöw B and Nestor M: Evaluation of cancer stem cell markers CD133, CD44, CD24: Association with AKT isoforms and radiation resistance in colon cancer cells. PLoS One. 9:e946212014. View Article : Google Scholar : PubMed/NCBI
15	Etemadmoghadam D and Bowtell D: AKT1 gene amplification as a biomarker of treatment response in ovarian cancer: Mounting evidence of a therapeutic target. Gynecol Oncol. 135:409–410. 2014. View Article : Google Scholar : PubMed/NCBI
16	Summers SA and Birnbaum MJ: A role for the serine/threonine kinase, Akt, in insulin-stimulated glucose uptake. Biochem Soc Trans. 25:981–988. 1997. View Article : Google Scholar : PubMed/NCBI
17	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
18	Hummel R, Sie C, Watson DI, Wang T, Ansar A, Michael MZ, Van der Hoek M, Haier J and Hussey DJ: MicroRNA signatures in chemotherapy resistant esophageal cancer cell lines. World J Gastroenterol. 20:14904–14912. 2014. View Article : Google Scholar : PubMed/NCBI
19	Xu X, Chen R, Li Z, Huang N, Wu X, Li S, Li Y and Wu S: MicroRNA-490-3p inhibits colorectal cancer metastasis by targeting TGFβR1. Bmc Cancer. 15:10232015. View Article : Google Scholar : PubMed/NCBI
20	Chen S, Chen X, Xiu YL, Sun KX and Zhao Y: MicroRNA-490-3P targets CDK1 and inhibits ovarian epithelial carcinoma tumorigenesis and progression. Cancer Lett. 362:122–130. 2015. View Article : Google Scholar : PubMed/NCBI
21	Wang LL, Sun KX, Wu DD, Xiu YL, Chen X, Chen S, Zong ZH, Sang XB, Liu Y and Zhao Y: DLEU1 contributes to ovarian carcinoma tumourigenesis and development by interacting with miR-490-3p and altering CDK1 expression. J Cell Mol Med. 21:3055–3065. 2017. View Article : Google Scholar : PubMed/NCBI
22	Liu W, Xu G, Liu H and Li T: MicroRNA-490-3p regulates cell proliferation and apoptosis by targeting HMGA2 in osteosarcoma. FEBS Lett. 589:3148–3153. 2015. View Article : Google Scholar : PubMed/NCBI
23	Sasaki T and Kuniyasu H: Significance of AKT in gastric cancer (Review). Int J Oncol. 45:2187–2192. 2014. View Article : Google Scholar : PubMed/NCBI
24	He J, Zhu G, Gao L, Chen P, Long Y, Liao S, Yi H, Yi W, Pei Z, Wu M, et al: Fra-1 is upregulated in gastric cancer tissues and affects the PI3K/Akt and p53 signaling pathway in gastric cancer. Int J Oncol. 47:1725–1734. 2015. View Article : Google Scholar : PubMed/NCBI