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Article

MicroRNA‑101 inhibits cell migration and invasion in bladder cancer via targeting FZD4

  • Authors:
    • Lei Chen
    • Yongqi Long
    • Zhijun Han
    • Zhizhou Yuan
    • Wenjin Liu
    • Fan Yang
    • Tao Li
    • Linfei Shu
    • Yunying Zhong
  • View Affiliations / Copyright

    Affiliations: Department of Urinary Surgery, Zhuzhou Central Hospital, Zhuzhou, Hunan 412000, P.R. China
  • Pages: 1476-1485
    |
    Published online on: December 11, 2018
       https://doi.org/10.3892/etm.2018.7084
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Abstract

Dysfunction of microRNAs (miRs) has been implicated in the development and progression of various human cancers. Our previous study demonstrated that miR‑101 inhibited bladder cancer cell proliferation and invasion through inhibition of c‑FOS expression. As an miR generally has many targets, other targets of miR‑101 may also serve important roles in bladder cancer progression. Reverse transcription‑quantitative polymerase chain reaction and western blot analyses were used to examine mRNA and protein expression, respectively. Wound healing and Transwell assays were conducted to study cell migration and invasion, respectively. The luciferase reporter gene assay was performed to verify one of the targets of miR‑101. The data in the present study indicate that the expression of miR‑101 is significantly reduced in bladder cancer tissues compared with that in adjacent non‑tumour tissues. In addition, miR‑101 expression is also downregulated in bladder cancer cell lines compared with that in normal bladder epithelial cells. Furthermore, low expression of miR‑101 was significantly associated with tumour metastasis, advanced clinical stage, and poor prognosis in bladder cancer. Frizzled class receptor 4 (FZD4) was identified as a novel target of miR‑101 in bladder cancer cells. The expression of FZD4 was significantly upregulated in bladder cancer tissues and cell lines. Both miR‑101 overexpression and FZD4 inhibition caused a significant reduction of the migration and invasion of bladder cancer cells, whereas overexpression of FZD4 reversed the suppressive effects of miR‑101 on bladder cancer cell migration and invasion. In conclusion, it was demonstrated that miR‑101 downregulation is associated with bladder cancer progression and that miR‑101 can inhibit bladder cancer cell migration and invasion via directly targeting FZD4. The present study expands the understanding of the molecular mechanisms underlying bladder cancer progression.
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Copy and paste a formatted citation
Spandidos Publications style
Chen L, Long Y, Han Z, Yuan Z, Liu W, Yang F, Li T, Shu L and Zhong Y: MicroRNA‑101 inhibits cell migration and invasion in bladder cancer via targeting FZD4. Exp Ther Med 17: 1476-1485, 2019.
APA
Chen, L., Long, Y., Han, Z., Yuan, Z., Liu, W., Yang, F. ... Zhong, Y. (2019). MicroRNA‑101 inhibits cell migration and invasion in bladder cancer via targeting FZD4. Experimental and Therapeutic Medicine, 17, 1476-1485. https://doi.org/10.3892/etm.2018.7084
MLA
Chen, L., Long, Y., Han, Z., Yuan, Z., Liu, W., Yang, F., Li, T., Shu, L., Zhong, Y."MicroRNA‑101 inhibits cell migration and invasion in bladder cancer via targeting FZD4". Experimental and Therapeutic Medicine 17.2 (2019): 1476-1485.
Chicago
Chen, L., Long, Y., Han, Z., Yuan, Z., Liu, W., Yang, F., Li, T., Shu, L., Zhong, Y."MicroRNA‑101 inhibits cell migration and invasion in bladder cancer via targeting FZD4". Experimental and Therapeutic Medicine 17, no. 2 (2019): 1476-1485. https://doi.org/10.3892/etm.2018.7084
Copy and paste a formatted citation
x
Spandidos Publications style
Chen L, Long Y, Han Z, Yuan Z, Liu W, Yang F, Li T, Shu L and Zhong Y: MicroRNA‑101 inhibits cell migration and invasion in bladder cancer via targeting FZD4. Exp Ther Med 17: 1476-1485, 2019.
APA
Chen, L., Long, Y., Han, Z., Yuan, Z., Liu, W., Yang, F. ... Zhong, Y. (2019). MicroRNA‑101 inhibits cell migration and invasion in bladder cancer via targeting FZD4. Experimental and Therapeutic Medicine, 17, 1476-1485. https://doi.org/10.3892/etm.2018.7084
MLA
Chen, L., Long, Y., Han, Z., Yuan, Z., Liu, W., Yang, F., Li, T., Shu, L., Zhong, Y."MicroRNA‑101 inhibits cell migration and invasion in bladder cancer via targeting FZD4". Experimental and Therapeutic Medicine 17.2 (2019): 1476-1485.
Chicago
Chen, L., Long, Y., Han, Z., Yuan, Z., Liu, W., Yang, F., Li, T., Shu, L., Zhong, Y."MicroRNA‑101 inhibits cell migration and invasion in bladder cancer via targeting FZD4". Experimental and Therapeutic Medicine 17, no. 2 (2019): 1476-1485. https://doi.org/10.3892/etm.2018.7084
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