Correlation between IL-4 and IL-13 gene polymorphisms and asthma in Uygur children in Xinjiang

Zhang,Ji-Hong; Zhang,Mei; Wang,Ya-Nan; Zhang,Xiao-Ying

doi:10.3892/etm.2018.7096

Correlation between IL-4 and IL-13 gene polymorphisms and asthma in Uygur children in Xinjiang

Authors:
- Ji-Hong Zhang
- Mei Zhang
- Ya-Nan Wang
- Xiao-Ying Zhang
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Affiliations: Department of Paediatrics, The Affiliated Yangpu Hospital of Shanghai Tongji University, Shanghai 200090, P.R. China, Department of Paediatrics, The First Affiliated Hospital of Xinjiang Medical University, Xinjiang, Uygur Autonomous Region 830054, P.R. China, Department of Paediatrics, Xinjiang Medical University, Xinjiang, Uygur Autonomous Region 830011, P.R. China, Department of Paediatrics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China
Published online on: December 13, 2018 https://doi.org/10.3892/etm.2018.7096
Pages: 1374-1382

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Abstract

Correlation between interleukin-4 (IL-4) and interleukin-13 (IL-13) gene polymorphisms and bronchial asthma in Uygur children in Xinjiang, China, and the effects on serum total immunoglobulin E (IgE) were investigated. Thirty-seven child patients with asthma and 29 healthy children were screened. The gene polymorphisms of Arg130Gln in IL-13 and -590C/T in IL-4 gene promoter region were analyzed using the polymerase chain reaction (PCR) and direct gene sequencing; the asthma-related indexes were detected using the enzyme-linked immunosorbent assay, and the relevant indexes were analyzed; moreover, whether there was a synergistic effect between Arg130Gln in IL-13 and -590C/T in IL-4 gene promoter region in the pathogenesis of asthma in children was analyzed. The T/T homozygote genotype frequency and T allele frequency of C-590T gene in IL-4 in the asthma group were significantly higher than those in the control group (45.9 vs. 10.3%, OR=8.91; 63.5 vs. 36.2%, OR=3.07, P<0.05). The A/A genotype frequency and A allele frequency of Arg130Gln locus in IL-13 in the asthma group were obviously higher than those in the control group (54.1 vs. 17.2%, OR=6.29; 67.6 vs. 39.7%, OR=3.17, P<0.05). In the two gene loci, the level of serum IgE in the same genotype in the asthma group was higher than that in the control group (Z=-2.128, -2.050, -2.700 vs. -3.766, -3.799, -3.397; P<0.05). The risk of asthma in carriers of both IL-4 -590C/T TT and Arg130Gln locus AA genotypes was significantly increased compared with that in carriers of either IL-4 -590C/T TT genotype or Arg130Gln locus AA genotype (OR=6.00, P=0.046; OR=4.50, P=0.033; OR=22, P=0.005). The IL-4 -590C/T and Arg130Gln locus gene polymorphisms are associated with the asthma susceptibility and increased serum total IgE in Uygur children in Xinjiang. There is a synergistic effect between the T allele of IL-4 -590C/T locus and the A allele of IL-13 Arg130Gln locus.

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