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Article

Metformin protects against sevoflurane‑induced neuronal apoptosis through the S1P1 and ERK signaling pathways

  • Authors:
    • Huiyu Yue
    • Bin Hu
    • Zhikai Luo
    • Mei Liu
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Shaan Xi Provincial Tumor Hospital, Xi'an, Shaanxi 710061, P.R. China, Department of Anesthesiology, Yan'an University Affiliated Hospital, Yan'an, Shaanxi 716000, P.R. China
  • Pages: 1463-1469
    |
    Published online on: December 13, 2018
       https://doi.org/10.3892/etm.2018.7098
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Abstract

The aim of the current study was to investigate whether metformin could counteract sevoflurane‑induced neurotoxicity. In vitro experiments on the sevoflurane‑induced nerve injury were performed using hippocampal neurons. Neuronal apoptosis was detected by an MTT assay. Protein expression levels of apoptosis‑associated genes, including cleaved‑caspase‑3, apoptosis regulator BAX and apoptosis regulator Bcl‑2 were detected by western blot analysis. The mechanism of the effect of metformin on sevoflurane‑induced neuronal apoptosis was investigated using a sphingosine 1‑phosphate receptor 1 (S1P1) antagonist (VPC23019) and mitogen‑activated protein kinase kinase inhibitor (U0126). The current study revealed that metformin may reduce sevoflurane‑induced neuronal apoptosis via activating mitogen‑activated protein kinase (ERK)1/2 phosphorylation. VPC23019 and U0126 eliminated the neuroprotective effects of metformin on neuronal apoptosis, which suggests that metformin is able to protect against sevoflurane‑induced neurotoxicity via activation of the S1P1‑dependent ERK1/2 signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Yue H, Hu B, Luo Z and Liu M: Metformin protects against sevoflurane‑induced neuronal apoptosis through the S1P1 and ERK signaling pathways. Exp Ther Med 17: 1463-1469, 2019.
APA
Yue, H., Hu, B., Luo, Z., & Liu, M. (2019). Metformin protects against sevoflurane‑induced neuronal apoptosis through the S1P1 and ERK signaling pathways. Experimental and Therapeutic Medicine, 17, 1463-1469. https://doi.org/10.3892/etm.2018.7098
MLA
Yue, H., Hu, B., Luo, Z., Liu, M."Metformin protects against sevoflurane‑induced neuronal apoptosis through the S1P1 and ERK signaling pathways". Experimental and Therapeutic Medicine 17.2 (2019): 1463-1469.
Chicago
Yue, H., Hu, B., Luo, Z., Liu, M."Metformin protects against sevoflurane‑induced neuronal apoptosis through the S1P1 and ERK signaling pathways". Experimental and Therapeutic Medicine 17, no. 2 (2019): 1463-1469. https://doi.org/10.3892/etm.2018.7098
Copy and paste a formatted citation
x
Spandidos Publications style
Yue H, Hu B, Luo Z and Liu M: Metformin protects against sevoflurane‑induced neuronal apoptosis through the S1P1 and ERK signaling pathways. Exp Ther Med 17: 1463-1469, 2019.
APA
Yue, H., Hu, B., Luo, Z., & Liu, M. (2019). Metformin protects against sevoflurane‑induced neuronal apoptosis through the S1P1 and ERK signaling pathways. Experimental and Therapeutic Medicine, 17, 1463-1469. https://doi.org/10.3892/etm.2018.7098
MLA
Yue, H., Hu, B., Luo, Z., Liu, M."Metformin protects against sevoflurane‑induced neuronal apoptosis through the S1P1 and ERK signaling pathways". Experimental and Therapeutic Medicine 17.2 (2019): 1463-1469.
Chicago
Yue, H., Hu, B., Luo, Z., Liu, M."Metformin protects against sevoflurane‑induced neuronal apoptosis through the S1P1 and ERK signaling pathways". Experimental and Therapeutic Medicine 17, no. 2 (2019): 1463-1469. https://doi.org/10.3892/etm.2018.7098
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