Involvement of gap junctions in propylthiouracil‑induced cytotoxicity in BRL‑3A cells

Introduction

The Food and Drug Administration (FDA) approved propylthiouracil (PTU) for the treatment of Graves' disease in 1947 (1). In nearly 70 years of clinical application, reports of PTU-associated liver injury and failure, and even fatality, have accumulated for adult and pediatric patients (2–6). A warning regarding the potential risk of severe hepatic injury associated with PTU was issued by the FDA in 2009 (7). Therefore, it is recommended that patients receiving PTU therapy have their liver function closely monitored. PTU-induced liver injury primarily manifests as differing degrees of hepatocyte necrosis (8); however, the underlying mechanisms are largely unknown.

Gap junctions (GJs) directly connect the cytoplasm of adjacent cells, mediating the intercellular transmission of signaling molecules. Six transmembrane connexin (Cx) monomers are arranged in a circle to form a hemichannel, and then two hemichannels from neighboring plasma membranes are docked to form the GJ (9,10). Cx expression is distinct in a variety of tissues, and Cx32 is the major GJ protein in hepatocytes (11,12).

GJ-mediated intercellular communication (GJIC) is involved in a number of physiological and pathological processes (13–15). Previous reports have suggested a role for GJ channels in drug-induced liver injury (DILI) (16–18). Downregulation of GJs composed of Cx32 (Cx32-GJs) could reduce the hepatotoxicity of acetaminophen, D-galactosamine and carbon tetrachloride (19,20). Likewise, propofol protects rat liver cells from sevoflurane-induced cytotoxicity through inhibiting GJ channels (21). Based on this evidence, the inhibition of hepatic Cx32-GJs could prove to be an effective strategy for controlling DILI. However, whether this GJ-mediated hepatoprotection is effective against PTU toxicity, and the potential underlying mechanism of this, remain unknown. In the present study, the role and underlying mechanisms of GJs in PTU-induced toxicity were explored in BRL-3A cells.

Materials and methods

Materials

PTU, carbenoxolone (CBX), anti-GAPDH and secondary antibodies for western blotting were obtained from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany). Anti-Cx32 antibody was obtained from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Cell culture reagents, Lipofectamine 2000 and calcein acetoxymethyl ester (Calcein-AM) were purchased from Thermo Fisher Scientific, Inc. (Waltham, MA, USA). The Cell Counting kit-8 (CCK-8) was obtained from Dojindo (Mashikimachi, Kumamoto, Japan). The 2′,7′-dichlorofluorescin diacetate (DCFH-DA) was from Beyotime Institute of Biotechnology (Haimen, China). All other reagents and chemicals were obtained from Sigma-Aldrich; Merck KGaA, unless otherwise stated.

Cell culture

The BRL-3A rat liver cell line was purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Cells were cultivated in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum and 100 U/ml penicillin-streptomycin at 37°C in an atmosphere containing 5% CO2.

CCK-8 assay

Direct toxicity was determined using a CCK-8 kit according to the manufacturer's instructions. First, BRL-3A cells were subjected to 0.6 and 0.8 mg/ml PTU for 24 h at 37°C, after which they were incubated with 10% (v/v) CCK-8 reagent at 37°C for 3 h. The absorbance was read using a microplate reader (BioTek Instruments, Inc., Winooski, VT, USA) at a wavelength of 450 nm. The cell viability was normalized against that of the vehicle control.

A standard colony-formation assay

A standard colony-formation assay was used for detecting the cytotoxicity of PTU to BRL-3A cells (22). Briefly, following exposure to PTU at 0.6 and 0.8 mg/ml for 12 h, cells were rinsed with phosphate-buffered saline (PBS), harvested with trypsin, diluted and seeded into 6-well plates at a density of 500 cells/well. Cells were subsequently stained with 4% crystal violet at room temperature 5–7 days later. Colonies consisting of ≥50 cells were counted. The surviving fraction was evaluated by normalizing to the colony-forming efficiency of the vehicle-treated cells.

Small interfering (si)RNA transfection

Cx32 expression was inhibited by siRNA transfection in BRL-3A cells. The siRNA sequences targeted against the rat Cx32 gene were as described previously (23,24): siRNA-1, 5′-CACCAACAACACATAGAAA-3′; siRNA-2, 5′-GCATCTGCATTATCCTCAA-3′; and siRNA-3, 5′-GCCTCTCACCTGAATACAA-3′. Cx32 siRNAs (50 nM) or the negative control siRNA (NC siRNA) were transiently transfected into BRL-3A cells using Lipofectamine 2000, according to the manufacturer's instructions. After 48 h incubation, western blotting and a ‘parachute’ assay, as described below, were performed to confirm Cx32 expression knockdown and GJIC inhibition.

Western blotting

The procedure of western blotting was performed as described previously (25). In brief, cell lysates were obtained in lysis buffer (P0013; Beyotime Institute of Biotechnology), sonicated and centrifuged at 14,167 × g at 4°C for 30 min. Protein concentration was determined by BCA assay. Cell lysates (20 µg per lane) were separated using SDS-PAGE in 10% Tris-glycine gels and transferred to nitrocellulose membranes. Following blocking with 5% skimmed dry milk in Tris buffered saline with Tween-20 (0.05% Tween-20) at room temperature for 1 h, the membranes were incubated with specific antibodies against Cx32 (sc-59948; dilution, 1:1,000) and GAPDH (G8795; dilution, 1:2,000) overnight at 4°C. Secondary antibodies (goat anti-mouse IgG-peroxidase conjugated; A4416; dilution, 1:4,000) were then added at room temperature for 1 h. The immunopositive protein bands were visualized using an Amersham Enhanced Chemiluminescence Detection kit (GE Healthcare Life Sciences, Little Chalfont, UK) and the intensities were detected by the Quantity One software (Bio-Rad, version 4.6.2).

‘Parachute’ dye-coupling assay

A ‘parachute’ dye-coupling assay was performed to evaluate GJ function as previously described (26). Cells were seeded into 12-well plates and cultured to 80–90% confluency. Donor cells from one well were labeled with 5 µM Calcein-AM and trypsinized, diluted and seeded onto receiver cells at a ratio of 1:150 (donor/receiver). GJs formed between donor cells and receiver cells during a 4-h incubation at 37°C and were monitored using an Olympus IX71 fluorescence microscope (Olympus Corporation, Tokyo, Japan). Under each experimental condition (treatment of CBX or (si)RNA transfection), the average number of receiver cells containing calcein dye per donor cell was counted and normalized to that of the vehicle control.

Observation of cell morphology

Cell morphology was observed using a FEI Quanta-400 scanning electron microscope (SEM; Thermo Fisher Scientific, Inc.). Sterile slides were placed in 12-well plates and served as substrates. Cells were treated with vehicle control or PTU at 0.6 and 0.8 mg/ml for 24 h, rinsed with PBS and fixed in 4% paraformaldehyde for 1 h at room temperature. Following gradient dehydration in a series of ethanol from 30–100%, the samples were dried in a vacuum freeze-drying apparatus for 1 h and were treated with gold sputtering for SEM observation.

Detection of PTU concentration in BRL-3A cells

Reversed-phase high-performance liquid chromatography was adopted to measure the PTU content in BRL-3A cells (27). Briefly, following incubation with PTU at 0.6 and 0.8 mg/ml for 24 h, cells were washed with PBS three times, harvested by trypsinization, resuspended in PBS and counted. Five freeze-thaw cycles were used to lyse the cells. Methanol (HPLC-grade) was added for protein precipitation. Following centrifugation at 14,167 × g for 10 min at 4°C, the supernatant was collected and injected into a Shimadzu LC-20AD system (Shimadzu Corporation, Kyoto, Japan). The chromatographic conditions used were as follows: Application of a Luna C18 column (250×4.6 mm; 5mm; Phenomenex, Torrance, CA, USA), methanol and water (40:60) was used as the mobile phase at a flow rate of 1.0 ml/min, which was detected at a wavelength of 272 nm with a column temperature of 30°C. The concentrations of PTU in the cell samples were calculated using a calibration curve method.

Analysis of intracellular reactive oxygen species (ROS) levels

Following exposure to PTU at 0.6 and 0.8 mg/ml for 6 h, BRL-3A cells were labeled with DCFH-DA, which is hydrolyzed by esterase into DCFH without fluorescence. Intracellular ROS can oxidize non-fluorescent DCFH to fluorescent DCF (28). Formation of DCF was determined using a Perkin LS55 fluorescence spectrophotometer (PerkinElmer Inc., Waltham, MA, USA) with an excitation wavelength of 488 nm and an emission wavelength of 525 nm. Fluorescence intensity was normalized to that of the vehicle control and was regarded as a measure of the intracellular ROS level. The DCF images were captured via fluorescence microscopy (Olympus IX71; Olympus Corporation).

Statistical analysis

The data were presented as the mean ± standard error and were analyzed using IBM SPSS Statistics 19.0 (Armonk, NY, USA). Statistical analysis was performed by Student's t-test or one-way analysis of variance followed by Dunnett's test. In all cases, P<0.05 was considered to indicate a statistically significant difference.

Results

Cell density influences the cytotoxic effect of PTU in BRL-3A cells

BRL-3A cells were cultured separately under two cell-density conditions for initial investigation of the influence of GJIC on PTU toxicity. At a high cell density (2×104 cells per cm2), GJs formed efficiently as the cells had frequent contact with one another. At a low cell density (5×103 cells per cm2), the wide dispersion of cells infrequently permitted the formation of GJs. A preliminary cell viability assay for concentrations screening was performed with PTU from 0.3 to 0.8 mg/ml (data not shown). The concentrations of 0.6 and 0.8 mg/ml PTU were chosen in the present study. PTU-induced cytotoxicity was assessed using a CCK-8 assay and a standard colony-formation assay. Fig. 1 illustrates that PTU treatment, at concentrations of 0.6 and 0.8 mg/ml, decreased the cell survival of BRL-3A cells under both culture conditions. However, the survival was significantly greater in the low-density condition compared with the high-density condition. Thus, the toxicity of PTU in BRL-3A cells was reduced under low cell-density conditions wherein there was a deficiency in GJIC.

Figure 1. Effect of cell density on PTU cytotoxicity in BRL-3A cells. Cell survival detected by (A) Cell Counting kit-8 and (B) colony formation assays at high and low cell densities, following treatment with 0.6 and 0.8 mg/ml PTU. *P<0.05 vs. high-density cell culture in (A); #P<0.05 vs. high-density cell culture in (B). PTU, propylthiouracil.

Pharmacological inhibition of GJ function decreases PTU cytotoxicity in BRL-3A cells

To investigate whether the cell density-dependence of PTU toxicity in BRL-3A cells is associated with GJIC, an extensive GJ inhibitor, CBX (29), was adopted to manipulate GJ function. BRL-3A cells were pretreated with the CBX prior to exposure to PTU. In a subsequent ‘parachute’ assay, dye-coupling was significantly blocked by CBX pretreatment (Fig. 2A). Under high cell-density culture conditions, the cell survival was significantly increased following pretreatment with 100 µM CBX for 1 h at PTU concentrations of 0.6 and 0.8 mg/ml, as compared with PTU alone (Fig. 2B and C). These results indicate that the inhibition of GJs contributes to the decreased toxicity of PTU at high BRL-3A cell densities.

Figure 2. Effect of CBX on PTU cytotoxicity in BRL-3A cells. (A) CBX suppressed the spread of dye between BRL-3A cells, as indicated by a ‘parachute’ assay (magnification, ×100). *P<0.05 vs. vehicle control. (B) Cell viability by Cell Counting kit-8 and the (C) survival fraction as well as the representative colony images by a standard colony-formation assay of BRL-3A cells incubated with 0.6 and 0.8 mg/ml PTU at a high cell density following pretreatment with CBX. *P<0.05 vs. 0.6 mg/ml PTU with GJ; #P<0.05 vs. 0.8 mg/ml PTU with GJ. CBX, carbenoxolone; PTU, propylthiouracil; GJ, gap junction.

Suppression of Cx32-GJ function by Cx32 knockdown attenuates PTU cytotoxicity

Cx32 is a key GJ constituent protein in liver cells (12). Specific knockdown of the Cx32 gene was conducted to confirm the effect of Cx32-GJ on PTU cytotoxicity. As indicated in Fig. 3A and B, the downregulation of Cx32 expression significantly suppressed the spread of calcein dye through GJs in siRNA-transfected cells. Furthermore, Cx32-knockdown (siRNA-1 transfection) increased the cell viability by factors of 1.31 and 1.29 in the presence of 0.6 and 0.8 mg/ml PTU, respectively, at high cell densities (Fig. 3C). Likewise, clonogenic capacity was improved at these two concentrations when Cx32-GJs were inhibited (Fig. 3D). The results indicate that Cx32-GJ serves an important role in the PTU-induced cytotoxicity of BRL-3A cells.

Figure 3. Effect of Cx32 siRNA-mediated inhibition of GJ function on PTU cytotoxicity in BRL-3A cells. (A and B) Western blot and ‘parachute’ dye-coupling assays (magnification, ×100) were adopted to measure the influence of siRNA on Cx32 expression and the degree of GJ intercellular communication. *P<0.05 vs. control. (C) Cell viability of BRL-3A cells exposed to PTU for 24 h and (D) survival fraction and the representative colony images of BRL-3A cells exposed to PTU for 12 h, each at a high cell density, following transfection with Cx32 siRNA-1. *P<0.05 vs. 0.6 mg/ml PTU with GJ; #P<0.05 vs. 0.8 mg/ml PTU with GJ. CBX, carbenoxolone; PTU, propylthiouracil; GJ, gap junction; siRNA, small interfering RNA; Cx32, connexin 32.

Cx32-GJs influence cell morphology and necrosis

PTU has been demonstrated to induce necrosis in liver injury (8). To assess the necrosis of BRL-3A cells subjected to PTU in the absence of Cx32-GJ, SEM images were captured. Fig. 4 illustrates the typical morphological characteristics of cell necrosis, including cell swelling and spillovers of cellular content (as indicated by the arrows), which were clearly noted following PTU treatment, but were less obvious following siRNA-mediated Cx32-GJ inhibition. These results suggest that the suppression Cx32-GJ attenuates PTU hepatotoxicity, which is partly associated with a decrease in PTU-induced necrosis.

Figure 4. Cx32 siRNA-1 modulates GJ function affecting PTU-induced cell morphological changes. Scanning electron microscope images showed the morphological changes to cells exposed to PTU for 24 h following transfection with Cx32 siRNA-1 (magnification, ×200). Arrows, typical morphological characteristics of cell necrosis. PTU, propylthiouracil; GJ, gap junction; siRNA, small interfering RNA; Cx32, connexin 32.

Inhibition of Cx32-GJ decreases intracellular PTU accumulation

GJs facilitate direct intercellular communication between neighboring cells. PTU, as a small molecule, could be transmitted through GJs. To assess whether the PTU concentration in BRL-3A cells could be reduced by blocking Cx32-GJ function, the intracellular PTU content was determined. As indicated in Fig. 5, the level of cellular PTU [PTU (µg) per 104 cells] significantly decreased when cells were transfected with Cx32 siRNA-1. The reduction of PTU uptake in BRL-3A cells between those with and without Cx32-GJ were significant at 0.6 and 0.8 mg/ml PTU. These results demonstrate that PTU accumulation is reduced when Cx32-GJ is downregulated.

Figure 5. Effect of connexin 32 siRNA-1-mediated GJ-inhibition on intracellular PTU content in BRL-3A cells. Cells were exposed to 0.6 and 0.8 mg/ml PTU for 24 h, and HPLC was performed to determine the intracellular PTU content. *P<0.05 vs. 0.6 mg/ml PTU with GJ; #P<0.05 vs. 0.8 mg/ml PTU with GJ. PTU, propylthiouracil; GJ, gap junction; siRNA, small interfering RNA.

Inhibition of Cx32-GJ prevents ROS transmission

To investigate the possibility of ROS transmission through GJs, intracellular ROS levels in the presence and absence of Cx32-GJ were detected. The results revealed that PTU-induced toxicity was accompanied by an increase in ROS production at concentrations ≤0.8 mg/ml. Fig. 6 illustrates that Cx32 siRNA-1 transfection markedly suppressed the PTU-induced increase in DCF fluorescence, which was quantified by fluorescence spectrophotometry. The levels of intracellular ROS were significantly reduced in the Cx32-knockdown group at PTU concentrations of 0.6 and 0.8 mg/ml. These findings suggest that the attenuation of PTU-induced toxicity by Cx32-GJ downregulation may be associated with the inhibition of ROS transmission.

Figure 6. Effect of connexin 32 siRNA-1-mediated GJ-inhibition on ROS levels in BRL-3A cells. Intracellular ROS levels were detected with a dichlorofluorescin assay, and images were obtained via fluorescence microscopy (magnification, ×100). Quantitative results were assessed according to the intensity of dichlorofluorescein fluorescent cells normalized to the control. *P<0.05 vs. 0.6 mg/ml PTU with GJ; #P<0.05 vs. 0.8 mg/ml PTU with GJ. PTU, propylthiouracil; GJ, gap junction; siRNA, small interfering RNA; ROS, reactive oxygen species.

Discussion

In the present study, it was demonstrated that PTU-induced toxicity depends on GJs in BRL-3A cells. The investigation indicated that the inhibition of GJs could protect against PTU-induced liver cell damage in vitro. Furthermore, PTU-induced necrosis was decreased following specific blocking of Cx32-GJs. Results from the current study illustrated that inhibition of GJ has a protective effect in PTU-induced liver toxicity and that GJ-attenuated cytotoxicity is associated, in part, with the reduction of necrosis.

Recent studies have demonstrated that the inhibition of hepatic Cx32-GJs can attenuate the liver toxicity associated with various types of drugs, suggesting a promising new therapeutic target for DILI (19,20). An ‘injury signal’ responsible for inducing apoptosis or necrosis diffuses through GJ channels to cause damage or death in the adjacent cells, which is known as the ‘bystander effect’. This effect has been observed in herpes simplex virus thymidine kinase and in the radiotherapy of cancer (30–32). In DILI, blocking the propagation of ‘injury signals’ among cells through Cx32-GJ may be key to this hepatoprotective effect. The ‘injury signals’ mediated by GJs have been widely investigated, particularly in carcinoma cells (25,33). However, their intrinsic properties have not been identified. The relevant signals are considered to be the drugs or their toxic metabolites, calcium, inositol 1,4,5-triphosphate (IP3), or other signaling molecules triggering cell damage (34–37).

There are ~21 Cx isoforms in mammals, which assemble into junction channels with selective permeability to molecules (9). For example, Cx32-GJs exhibit higher permeability to adenosine, cAMP and IP3 than do Cx43-GJs, while ATP is conducted through Cx43-GJs more effectively than through Cx32-GJs (38–40). In the present work, inhibition of GJ function using extensive GJ blocker (CBX) and Cx32 siRNAs decreases PTU cytotoxicity, in which Cx32-GJ have an essential role on this hepatoprotective effect. Therefore, in the present study, the possible ‘injury signals’ transmitted through Cx32-GJs should have the following characteristics: i) The ability to permeate through Cx32 channels; and ii) the ability to influence the cytotoxicity of PTU. It was considered that PTU and ROS are the potential ‘injury signals’ involved in Cx32-GJ-mediated PTU toxicity.

The molecular weight of PTU is 170.2 Da, which is less than the limit for passage through GJ channels (1–2 kDa); therefore, it could be transferred via GJs by passive diffusion. Furthermore, PTU could markedly suppress glutathione S-transferase (GST) activity to interfere with the antioxidation of glutathione (41). Therefore, the present study was performed to investigate the possibility that PTU is a GJ-permeable ‘injury signal’. The results indicated that PTU uptake was decreased in cells deficient in functional Cx32 channels. This decline may not only be conducive to the restoration of GST activity; it may also weaken allergic responses caused by PTU acting as a hapten.

Oxidative stress was observed in hypothyroid rats induced by administration of drinking water containing 0.05% PTU for 8 weeks (42,43). Oxidative stress is an important pathophysiological basis of liver injury, in which ROS is the essential mediator; it induces lipid peroxidation of the cellular and mitochondrial membranes, causing changes in permeability and affecting signal transduction pathways (e.g., ROS/Protein kinase C, ROS/c-Jun N-terminal kinase (44–46). Previous studies identified that ROS can spread through Cx32 channels, acting as toxic molecules (19,47). The experimental results in the present study suggested that ROS induced by PTU-exposure were regulated by Cx32-GJs. Rat hepatocytes deficient in Cx32 could prevent the passage of ROS between cells. Thus, ROS could be PTU-induced ‘injury signals’ with selective Cx32 channel permeability.

Immortalized BRL-3A cells endogenously expressing Cx32 and Cx43 is a simple model for investigating the role of GJ in PTU-induced hepatotoxicity in vitro. However, the limitations of using only one rat liver cell line are the differences in species and primary hepatocytes (such as confounding factors arising from the complicated hemodynamic system), the effects of GJs on the PTU-induced cytotoxicity in human hepatocytes, and in vivo, remain to be explored.

In conclusion, the role of GJs in PTU-induced cytotoxicity was investigated in BRL-3A cells. The results indicated that the suppression of Cx32-GJ channels protected against PTU-cytotoxicity through reducing necrosis. Furthermore, PTU and ROS appeared to affect the Cx32-GJ-mediated hepatoprotective effect, acting as ‘injury signals’.

Acknowledgements

Not applicable.

Funding

The present study was supported by the National Natural Science Foundation of China (grant no. 81400619), the Scientific Research Project of Education Department of Guangdong Province (grant no. 2017KTSCX079), the Funding for College Students' Science and Technology Innovation and Cultivation of Guangdong Province (grant no. pdjhb0220).

Availability of data and materials

All data generated or analyzed during this study are included in this published article.

Authors' contributions

NT designed the current study. NT, ZC, HC, LC and BC performed the experiments. NT, ZC and BL analyzed the data. All authors have read and approved the final manuscript.

Ethics approval and consent to participate

Not applicable.

Patient consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

References