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Penehyclidine hydrochloride exerts protective effects in rats with acute lung injury via the Fas/FasL signaling pathway

  • Authors:
    • Qian Kong
    • Xiaojing Wu
    • Weina Duan
    • Liying Zhan
    • Xuemin Song
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China, Department of Anesthesiology and Critical Care Medicine, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China
    Copyright: © Kong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3598-3606
    |
    Published online on: March 4, 2019
       https://doi.org/10.3892/etm.2019.7340
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Abstract

Acute lung injury (ALI) is a critical syndrome that is associated with high morbidity and mortality rates. The activation of the Fas/Fas ligand (FasL) signaling pathway may be an important pathophysiological mechanism during ALI development. Penehyclidine hydrochloride (PHC) has been revealed to exhibit anti‑apoptotic properties and may attenuate the observed systemic inflammatory response. The present study was performed to elucidate the molecular mechanism of PHC in the regulation of the Fas/FasL signaling pathway in rats with ALI. An ALI rat model was constructed by inducing blunt chest trauma and hemorrhagic shock (T/HS), with PHC administration prior to or following T/HS. At 6 h following T/HS, blood samples and lung tissues were collected. Western blotting, arterial blood gas analysis, ELISA, hematoxylin and eosin staining, terminal deoxynucleotidyl transferase‑­mediated dUTP nick‑end labeling staining and biochemical indicator analysis were performed to determine the degree of lung injury and the key signaling pathways associated with lung damage. The results indicated that the administration of PHC following T/HS effectively attenuates lung injury by improving pulmonary oxygenation, decreasing histopathological damage, decreasing polymorphonuclear neutrophil count and decreasing Fas, FasL, caspase‑8, caspase‑3, tumor necrosis factor‑α, interleukin (IL)‑6 and IL‑1β expression. The results indicated that PHC exhibits anti‑apoptotic functions and exerts protective effects in ALI rats induced by T/HS, which may be attributed to the inhibition of the Fas/FasL signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Kong Q, Wu X, Duan W, Zhan L and Song X: Penehyclidine hydrochloride exerts protective effects in rats with acute lung injury via the Fas/FasL signaling pathway. Exp Ther Med 17: 3598-3606, 2019.
APA
Kong, Q., Wu, X., Duan, W., Zhan, L., & Song, X. (2019). Penehyclidine hydrochloride exerts protective effects in rats with acute lung injury via the Fas/FasL signaling pathway. Experimental and Therapeutic Medicine, 17, 3598-3606. https://doi.org/10.3892/etm.2019.7340
MLA
Kong, Q., Wu, X., Duan, W., Zhan, L., Song, X."Penehyclidine hydrochloride exerts protective effects in rats with acute lung injury via the Fas/FasL signaling pathway". Experimental and Therapeutic Medicine 17.5 (2019): 3598-3606.
Chicago
Kong, Q., Wu, X., Duan, W., Zhan, L., Song, X."Penehyclidine hydrochloride exerts protective effects in rats with acute lung injury via the Fas/FasL signaling pathway". Experimental and Therapeutic Medicine 17, no. 5 (2019): 3598-3606. https://doi.org/10.3892/etm.2019.7340
Copy and paste a formatted citation
x
Spandidos Publications style
Kong Q, Wu X, Duan W, Zhan L and Song X: Penehyclidine hydrochloride exerts protective effects in rats with acute lung injury via the Fas/FasL signaling pathway. Exp Ther Med 17: 3598-3606, 2019.
APA
Kong, Q., Wu, X., Duan, W., Zhan, L., & Song, X. (2019). Penehyclidine hydrochloride exerts protective effects in rats with acute lung injury via the Fas/FasL signaling pathway. Experimental and Therapeutic Medicine, 17, 3598-3606. https://doi.org/10.3892/etm.2019.7340
MLA
Kong, Q., Wu, X., Duan, W., Zhan, L., Song, X."Penehyclidine hydrochloride exerts protective effects in rats with acute lung injury via the Fas/FasL signaling pathway". Experimental and Therapeutic Medicine 17.5 (2019): 3598-3606.
Chicago
Kong, Q., Wu, X., Duan, W., Zhan, L., Song, X."Penehyclidine hydrochloride exerts protective effects in rats with acute lung injury via the Fas/FasL signaling pathway". Experimental and Therapeutic Medicine 17, no. 5 (2019): 3598-3606. https://doi.org/10.3892/etm.2019.7340
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