Functional recombinant single‑chain variable fragment antibody against Agkistrodon acutus venom

  • Authors:
    • Lei Zhang
    • Yuliang Cao
    • Minghua Liu
    • Xiangyu Chen
    • Qiang Xiang
    • Jun Tian
  • View Affiliations

  • Published online on: March 6, 2019     https://doi.org/10.3892/etm.2019.7348
  • Pages: 3768-3774
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Agkistrodon acutus bites are conventionally treated with animal‑derived antivenom, the use of which is limited due to allergic reactions and serum sickness. Thus in the present study, the genes of humanized antibodies produced in response to A. acutus venom were extracted from lymphocytes from patients bitten by A. acutus. A single‑chain variable fragment (scFv) library against venom was constructed using a T7 phage display system. ScFv genes that exhibited high affinity to venom were selected by library biopanning. An expression system was constructed for antivenom scFv fused with 6xHis tag at its N‑ and C‑terminus using pET‑28a (+) vector. The scFv proteins could achieve functional and soluble expression in Escherichia coli via the auto‑induction method. The purity and activity of the scFv genes and proteins were confirmed by SDS‑PAGE, western blotting and ELISA. The results indicated that three soluble scFv proteins exhibited specific affinity to A. acutus venom and were harvested via the auto‑induction method.
View Figures
View References

Related Articles

Journal Cover

May-2019
Volume 17 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhang L, Cao Y, Liu M, Chen X, Xiang Q and Tian J: Functional recombinant single‑chain variable fragment antibody against Agkistrodon acutus venom. Exp Ther Med 17: 3768-3774, 2019
APA
Zhang, L., Cao, Y., Liu, M., Chen, X., Xiang, Q., & Tian, J. (2019). Functional recombinant single‑chain variable fragment antibody against Agkistrodon acutus venom. Experimental and Therapeutic Medicine, 17, 3768-3774. https://doi.org/10.3892/etm.2019.7348
MLA
Zhang, L., Cao, Y., Liu, M., Chen, X., Xiang, Q., Tian, J."Functional recombinant single‑chain variable fragment antibody against Agkistrodon acutus venom". Experimental and Therapeutic Medicine 17.5 (2019): 3768-3774.
Chicago
Zhang, L., Cao, Y., Liu, M., Chen, X., Xiang, Q., Tian, J."Functional recombinant single‑chain variable fragment antibody against Agkistrodon acutus venom". Experimental and Therapeutic Medicine 17, no. 5 (2019): 3768-3774. https://doi.org/10.3892/etm.2019.7348