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Effect of T‑cadherin on the AKT/mTOR signaling pathway, gastric cancer cell cycle, migration and invasion, and its association with patient survival rate

  • Authors:
    • Jianqing Lin
    • Zhiyao Chen
    • Zhijun Huang
    • Feng Chen
    • Zeyi Ye
    • Shaoze Lin
    • Weidong Wang
  • View Affiliations / Copyright

    Affiliations: Department of Surgical Oncology, Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian 362000, P.R. China
    Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3607-3613
    |
    Published online on: March 6, 2019
       https://doi.org/10.3892/etm.2019.7350
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Abstract

Gastric cancer (GC) is among the most common types of human cancer and is associated with recurrence and metastasis, despite comprehensive surgical and medical treatment. Previous studies observed downregulation of T‑cadherin expression in GC tissues, suggesting that this protein may act as an oncosuppressor. The current study investigated the activity of T‑cadherin in GC tissues. In a follow‑up study of 81 patients with GC, a Kaplan‑Meier analysis of overall survival revealed a strong association of T‑cadherin overexpression with increased overall survival (P<0.01). Furthermore, stable T‑cadherin‑overexpressing cell lines were established from HGC‑27 cells via transfection of a pcDNA3.1‑T‑cadherin plasmid and in vitro growth and cell cycle of these cells were measured using MTT and flow cytometry assays, respectively. MTT assays revealed that proliferation of engineered T‑cadherin‑overexpressing cells was significantly inhibited and flow cytometry demonstrated that T‑cadherin overexpression in HGC‑27 cells induced cell cycle arrest in the G0/G1 phase. Transwell assays demonstrated that T‑cadherin‑overexpressing HGC‑27 cells exhibited reduced invasiveness and metastatic potential. Phosphorylated (p)‑protein kinase B (AKT) and p‑mammalian target of rapamycin (mTOR) protein levels were reduced in T‑cadherin overexpressing HGC‑27 cells, suggesting that the AKT/mTOR signaling pathway was involved in the gastric tumor inhibitory effect of T‑cadherin. Administration of AKT‑activator, insulin‑like growth factor‑1, to T‑cadherin‑overexpressing HGC‑27 cells significantly affected the proliferation phenotype. In conclusion, the current study provided clinical evidence and revealed a potential mechanism supporting that T‑cadherin inhibits gastric tumorigenesis through inhibition of the AKT/mTOR signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Lin J, Chen Z, Huang Z, Chen F, Ye Z, Lin S and Wang W: Effect of T‑cadherin on the AKT/mTOR signaling pathway, gastric cancer cell cycle, migration and invasion, and its association with patient survival rate. Exp Ther Med 17: 3607-3613, 2019.
APA
Lin, J., Chen, Z., Huang, Z., Chen, F., Ye, Z., Lin, S., & Wang, W. (2019). Effect of T‑cadherin on the AKT/mTOR signaling pathway, gastric cancer cell cycle, migration and invasion, and its association with patient survival rate. Experimental and Therapeutic Medicine, 17, 3607-3613. https://doi.org/10.3892/etm.2019.7350
MLA
Lin, J., Chen, Z., Huang, Z., Chen, F., Ye, Z., Lin, S., Wang, W."Effect of T‑cadherin on the AKT/mTOR signaling pathway, gastric cancer cell cycle, migration and invasion, and its association with patient survival rate". Experimental and Therapeutic Medicine 17.5 (2019): 3607-3613.
Chicago
Lin, J., Chen, Z., Huang, Z., Chen, F., Ye, Z., Lin, S., Wang, W."Effect of T‑cadherin on the AKT/mTOR signaling pathway, gastric cancer cell cycle, migration and invasion, and its association with patient survival rate". Experimental and Therapeutic Medicine 17, no. 5 (2019): 3607-3613. https://doi.org/10.3892/etm.2019.7350
Copy and paste a formatted citation
x
Spandidos Publications style
Lin J, Chen Z, Huang Z, Chen F, Ye Z, Lin S and Wang W: Effect of T‑cadherin on the AKT/mTOR signaling pathway, gastric cancer cell cycle, migration and invasion, and its association with patient survival rate. Exp Ther Med 17: 3607-3613, 2019.
APA
Lin, J., Chen, Z., Huang, Z., Chen, F., Ye, Z., Lin, S., & Wang, W. (2019). Effect of T‑cadherin on the AKT/mTOR signaling pathway, gastric cancer cell cycle, migration and invasion, and its association with patient survival rate. Experimental and Therapeutic Medicine, 17, 3607-3613. https://doi.org/10.3892/etm.2019.7350
MLA
Lin, J., Chen, Z., Huang, Z., Chen, F., Ye, Z., Lin, S., Wang, W."Effect of T‑cadherin on the AKT/mTOR signaling pathway, gastric cancer cell cycle, migration and invasion, and its association with patient survival rate". Experimental and Therapeutic Medicine 17.5 (2019): 3607-3613.
Chicago
Lin, J., Chen, Z., Huang, Z., Chen, F., Ye, Z., Lin, S., Wang, W."Effect of T‑cadherin on the AKT/mTOR signaling pathway, gastric cancer cell cycle, migration and invasion, and its association with patient survival rate". Experimental and Therapeutic Medicine 17, no. 5 (2019): 3607-3613. https://doi.org/10.3892/etm.2019.7350
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