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Article Open Access

LncRNA ZEB1‑AS1 reduces liver cancer cell proliferation by targeting miR‑365a‑3p

  • Authors:
    • Mingfei Li
    • Hua Guan
    • Yuping Liu
    • Xianfeng Gan
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, Sichuan Provincial People's Hospital, Chengdu, Sichuan 610072, P.R. China, Health Management Centre, Sichuan Provincial People's Hospital, Chengdu, Sichuan 610072, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3539-3547
    |
    Published online on: March 7, 2019
       https://doi.org/10.3892/etm.2019.7358
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Abstract

Liver carcinoma is one of the most common malignancies worldwide. Previous studies have demonstrated that long non‑coding RNAs (lncRNAs) are crucial mediators that participate in a wide range of molecular processes associated with carcinogenesis. However, little is known about the specific mechanisms that underlie the majority of lncRNAs. Many studies have indicated that lncRNAs affect microRNA (miRNA or miR) activities via physical base‑paired binding, therefore serving as competing endogenous RNAs (ceRNAs) that indirectly regulate the expression of miRNA targets. In the current study, it was revealed that lncRNA zinc‑finger E‑box binding homeobox 1 antisense 1 (ZEB1‑AS1) serves as a ceRNA for miR‑365a‑3p, functioning to positively modulate E2F transcription factor 2 (E2F2) expression in liver cancer cells. Additionally, reverse transcription‑quantitative polymerase chain reaction demonstrated that levels of ZEB1‑AS1 were abnormally upregulated in liver cancer and this was positively correlated with E2F2 expression. Furthermore, high levels of ZEB1‑AS1 exhibited a trend for poor survival in patients with liver cancer. Western blot analysis demonstrated that ZEB1‑AS1 silencing could reduce E2F2 expression. EdU staining and flow cytometry analysis indicated that downregulation of ZEB1‑AS1 could suppress cell proliferation and decrease the S phase proportion of liver cancer cells, which was effectively reversed by the inhibition of miR‑365a‑3p. ZEB1‑AS1 was also determined to be physically associated with miR‑365a‑3p, while miR‑365a‑3p was revealed to target the E2F2 3'UTR for degradation or translational repression. The results also demonstrated that ZEB1‑AS1 positively regulates E2F2 expression by competitively binding to miR‑365a‑3p. It was further revealed to enhance liver cancer cell proliferation. Thus, these results indicate that ZEB1‑AS1 is required for liver cancer progression in a ceRNA dependent manner. ZEB1‑AS1 may therefore be a potential target for liver cancer intervention.
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1 

Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J and Jemal A: Global cancer statistics, 2012. CA Cancer J Clin. 65:87–108. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Li L and Wang H: Heterogeneity of liver cancer and personalized therapy. Cancer Lett. 379:191–197. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Forner A, Llovet JM and Bruix J: Hepatocellular carcinoma. Lancet. 379:1245–1255. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Batista PJ and Chang HY: Long noncoding RNAs: Cellular address codes in development and disease. Cell. 152:1298–1307. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Guttman M and Rinn JL: Modular regulatory principles of large non-coding RNAs. Nature. 482:339–346. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Wapinski O and Chang HY: Long noncoding RNAs and human disease. Trends Cell Biol. 21:354–361. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Geisler S and Coller J: RNA in unexpected places: Long non-coding RNA functions in diverse cellular contexts. Nat Rev Mol Cell Biol. 14:699–712. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Yang Y, Chen L, Gu J, Zhang H, Yuan J, Lian Q, Lv G, Wang S, Wu Y, Yang YT, et al: Recurrently deregulated lncRNAs in hepatocellular carcinoma. Nat Commun. 8:144212017. View Article : Google Scholar : PubMed/NCBI

9 

Esposti DD, Hernandez-Vargas H, Voegele C, Fernandez-Jimenez N, Forey N, Bancel B, Le Calvez-Kelm F, McKay J, Merle P and Herceg Z: Identification of novel long non-coding RNAs deregulated in hepatocellular carcinoma using RNA-sequencing. Oncotarget. 7:31862–31877. 2016. View Article : Google Scholar : PubMed/NCBI

10 

Cui H, Zhang Y, Zhang Q, Chen W, Zhao H and Liang J: A comprehensive genome-wide analysis of long noncoding RNA expression profile in hepatocellular carcinoma. Cancer Med. 6:2932–2941. 2017. View Article : Google Scholar : PubMed/NCBI

11 

Seitz H, Youngson N, Lin S, Dalbert S, Paulsen M, Bachellerie JP, Ferguson-Smith AC and Cavaille J: Imprinted microRNA genes transcribed antisense to a reciprocally imprinted retrotransposon-like gene. Nat Genet. 34:261–262. 2003. View Article : Google Scholar : PubMed/NCBI

12 

Tay Y, Rinn J and Pandolfi PP: The multilayered complexity of ceRNA crosstalk and competition. Nature. 505:344–352. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Salmena L, Poliseno L, Tay Y, Kats L and Pandolfi PP: A ceRNA hypothesis: The Rosetta stone of a hidden RNA language? Cell. 146:353–358. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Luo J, Qu J, Wu DK, Lu ZL, Sun YS and Qu Q: Long non-coding RNAs: A rising biotarget in colorectal cancer. Oncotarget. 8:22187–22202. 2017.PubMed/NCBI

15 

Qi X, Zhang DH, Wu N, Xiao JH, Wang X and Ma W: ceRNA in cancer: Possible functions and clinical implications. J Med Genet. 52:710–718. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Liu C and Lin J: Long noncoding RNA ZEB1-AS1 acts as an oncogene in osteosarcoma by epigenetically activating ZEB1. Am J Transl Res. 8:4095–4105. 2016.PubMed/NCBI

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Wang K, Liu CY, Zhou LY, Wang JX, Wang M, Zhao B, Zhao WK, Xu SJ, Fan LH, Zhang XJ, et al: APF lncRNA regulates autophagy and myocardial infarction by targeting miR-188-3p. Nat Commun. 6:67792015. View Article : Google Scholar : PubMed/NCBI

19 

Li M, Yang Y, Kuang Y, Gan X, Zeng W, Liu Y and Guan H: miR-365 induces hepatocellular carcinoma cell apoptosis through targeting Bcl-2. Exp Ther Med. 13:2279–2285. 2017. View Article : Google Scholar : PubMed/NCBI

20 

Li J, Li Z, Leng K, Xu Y, Ji D, Huang L, Cui Y and Jiang X: ZEB1-AS1: A crucial cancer-related long non-coding RNA. Cell Prolif. 51:e124232018. View Article : Google Scholar

21 

Li T, Xie J, Shen C, Cheng D, Shi Y, Wu Z, Deng X, Chen H, Shen B, Peng C, et al: Upregulation of long noncoding RNA ZEB1-AS1 promotes tumor metastasis and predicts poor prognosis in hepatocellular carcinoma. Oncogene. 35:1575–1584. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Lv QL, Hu L, Chen SH, Sun B, Fu ML, Qin CZ, Qu Q, Wang GH, He CJ and Zhou HH: A long noncoding RNA ZEB1-AS1 promotes tumorigenesis and predicts poor prognosis in glioma. Int J Mol Sci. 17:E14312016. View Article : Google Scholar : PubMed/NCBI

23 

Wang YL, Bai Y, Yao WJ, Guo L and Wang ZM: Expression of long non-coding RNA ZEB1-AS1 in esophageal squamous cell carcinoma and its correlation with tumor progression and patient survival. Int J Clin Exp Pathol. 8:11871–11876. 2015.PubMed/NCBI

24 

Xiong WC, Han N, Wu N, Zhao KL, Han C, Wang HX, Ping GF, Zheng PF, Feng H, Qin L and He P: Interplay between long noncoding RNA ZEB1-AS1 and miR-101/ZEB1 axis regulates proliferation and migration of colorectal cancer cells. Am J Transl Res. 10:605–617. 2018.PubMed/NCBI

25 

Ni Y, Fang J, Zhu L, Jiang H, Liu Y, Miao R, Shao C and Shao S: The significant prognostic value of ZEB1-AS1 up-regulation in patients with cancer. J Cancer. 9:2502–2509. 2018. View Article : Google Scholar : PubMed/NCBI

26 

Wang Y, Xu C, Wang Y and Zhang X: MicroRNA-365 inhibits ovarian cancer progression by targeting Wnt5a. Am J Cancer Res. 7:1096–1106. 2017.PubMed/NCBI

27 

Nie J, Liu L, Zheng W, Chen L, Wu X, Xu Y, Du X and Han W: microRNA-365, down-regulated in colon cancer, inhibits cell cycle progression and promotes apoptosis of colon cancer cells by probably targeting Cyclin D1 and Bcl-2. Carcinogenesis. 33:220–225. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Chen HZ, Tsai SY and Leone G: Emerging roles of E2Fs in cancer: An exit from cell cycle control. Nat Rev Cancer. 9:785–797. 2009. View Article : Google Scholar : PubMed/NCBI

29 

Reimer D, Sadr S, Wiedemair A, Goebel G, Concin N, Hofstetter G, Marth C and Zeimet AG: Expression of the E2F family of transcription factors and its clinical relevance in ovarian cancer. Ann N Y Acad Sci. 1091:270–281. 2006. View Article : Google Scholar : PubMed/NCBI

30 

Wang H, Zhang X, Liu Y, Ni Z, Lin Y, Duan Z, Shi Y, Wang G and Li F: Downregulated miR-31 level associates with poor prognosis of gastric cancer and its restoration suppresses tumor cell malignant phenotypes by inhibiting E2F2. Oncotarget. 7:36577–36589. 2016.PubMed/NCBI

31 

Nguyen-Vu T, Vedin LL, Liu K, Jonsson P, Lin JZ, Candelaria NR, Candelaria LP, Addanki S, Williams C, Gustafsson JA, et al: Liver × receptor ligands disrupt breast cancer cell proliferation through an E2F-mediated mechanism. Breast Cancer Res. 15:R512013. View Article : Google Scholar : PubMed/NCBI

32 

Chen L, Yu JH, Lu ZH and Zhang W: E2F2 induction in related to cell proliferation and poor prognosis in non-small cell lung carcinoma. Int J Clin Exp Pathol. 8:10545–10554. 2015.PubMed/NCBI

33 

Zhan L, Huang C, Meng XM, Song Y, Wu XQ, Miu CG, Zhan XS and Li J: Promising roles of mammalian E2Fs in hepatocellular carcinoma. Cell Signal. 26:1075–1081. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Li M, Guan H, Liu Y and Gan X: LncRNA ZEB1‑AS1 reduces liver cancer cell proliferation by targeting miR‑365a‑3p. Exp Ther Med 17: 3539-3547, 2019.
APA
Li, M., Guan, H., Liu, Y., & Gan, X. (2019). LncRNA ZEB1‑AS1 reduces liver cancer cell proliferation by targeting miR‑365a‑3p. Experimental and Therapeutic Medicine, 17, 3539-3547. https://doi.org/10.3892/etm.2019.7358
MLA
Li, M., Guan, H., Liu, Y., Gan, X."LncRNA ZEB1‑AS1 reduces liver cancer cell proliferation by targeting miR‑365a‑3p". Experimental and Therapeutic Medicine 17.5 (2019): 3539-3547.
Chicago
Li, M., Guan, H., Liu, Y., Gan, X."LncRNA ZEB1‑AS1 reduces liver cancer cell proliferation by targeting miR‑365a‑3p". Experimental and Therapeutic Medicine 17, no. 5 (2019): 3539-3547. https://doi.org/10.3892/etm.2019.7358
Copy and paste a formatted citation
x
Spandidos Publications style
Li M, Guan H, Liu Y and Gan X: LncRNA ZEB1‑AS1 reduces liver cancer cell proliferation by targeting miR‑365a‑3p. Exp Ther Med 17: 3539-3547, 2019.
APA
Li, M., Guan, H., Liu, Y., & Gan, X. (2019). LncRNA ZEB1‑AS1 reduces liver cancer cell proliferation by targeting miR‑365a‑3p. Experimental and Therapeutic Medicine, 17, 3539-3547. https://doi.org/10.3892/etm.2019.7358
MLA
Li, M., Guan, H., Liu, Y., Gan, X."LncRNA ZEB1‑AS1 reduces liver cancer cell proliferation by targeting miR‑365a‑3p". Experimental and Therapeutic Medicine 17.5 (2019): 3539-3547.
Chicago
Li, M., Guan, H., Liu, Y., Gan, X."LncRNA ZEB1‑AS1 reduces liver cancer cell proliferation by targeting miR‑365a‑3p". Experimental and Therapeutic Medicine 17, no. 5 (2019): 3539-3547. https://doi.org/10.3892/etm.2019.7358
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