miR‑552‑5p facilitates osteosarcoma cell proliferation and metastasis by targeting WIF1

Cai,Wei; Xu,Yong; Yin,Jian; Zuo,Wenshan; Su,Zhen

doi:10.3892/etm.2019.7361

miR‑552‑5p facilitates osteosarcoma cell proliferation and metastasis by targeting WIF1

Authors:
- Wei Cai
- Yong Xu
- Jian Yin
- Wenshan Zuo
- Zhen Su
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Affiliations: Orthopedics Department, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University, Huai'an, Jiangsu 223300, P.R. China, Orthopedics Department, The Affiliated Jiangning Hospital, Nanjing Medical University, Nanjing, Jiangsu 211100, P.R. China, Anesthesiology Department, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University, Huai'an, Jiangsu 223300, P.R. China
Published online on: March 7, 2019 https://doi.org/10.3892/etm.2019.7361
Pages: 3781-3788

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Abstract

miR‑552 promotes tumor growth and metastasis in colorectal cancer. However, the function of miR‑552 in osteosarcoma remains unclear. The current study investigated the role and mechanism of miR‑552‑5p in the proliferation, migration and invasion of osteosarcoma cells. miR‑552‑5p was significantly upregulated in osteosarcoma tissues and cell lines compared with adjacent normal tissues and normal osteoblast cells. Knockdown of miR‑552‑5p significantly reduced the proliferation of MG63 and U2OS cells, and inhibited cell migration and invasion. Wnt inhibitory factor 1 (WIF1) was the direct target gene of miR‑552‑5p in osteosarcoma cells. Overexpression of miR‑552‑5p markedly decreased the expression of WIF1 in MG63 and U2OS cells. A negative association was identified between the expression levels of miR‑552‑5p and WIF1 in osteosarcoma tissues. Furthermore, the expression of WIF1 was downregulated in osteosarcoma tissues and cell lines. Finally, knockdown of WIF1 in MG63 and U2OS cells treated with miR‑552‑5p inhibitors rescued their ability to proliferate, migrate and invade. Overall, the results indicated that miR‑552‑5p promoted osteosarcoma development and progression by inhibiting WIF1. Therefore, miR‑552‑5p may serve as a new therapeutic target for treatment of patients with osteosarcoma.

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