Open Access

Intranasal administration of Lignosus rhinocerotis (Cooke) Ryvarden (Tiger Milk mushroom) extract attenuates airway inflammation in murine model of allergic asthma

  • Authors:
    • Siti Aminah Muhamad
    • Nurul Syafira Muhammad
    • Nurul Dinie Ayuni Ismail
    • Rohimah Mohamud
    • Sabreena Safuan
    • Asma‑Abdullah Nurul
  • View Affiliations

  • Published online on: March 20, 2019     https://doi.org/10.3892/etm.2019.7416
  • Pages: 3867-3876
  • Copyright: © Muhamad et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Asthma is a chronic inflammatory disorder in the airways that involves the activation of cells and mediators. Lignosus rhinocerotis (Cooke) Ryvardan or Tiger Milk mushroom is a medicinal mushroom that is traditionally used to treat inflammatory diseases including asthma. In this study, the protective effects of intranasal administration of L. rhinocerotis extract (LRE) in ovalbumin (OVA)‑induced airway inflammation mouse model were investigated. Mice were sensitized by intraperitoneal (i.p) injection on days 0 and 14, followed by a daily challenge with 1% OVA from days 21 to 27. Following OVA challenge, LRE and dexamethasone were administered via intranasal and i.p. injection respectively. On day 28, the level of serum immunoglobulin (Ig)E, differential cell counts and T‑helper (Th) 2 cytokines in bronchoalveolar lavage fluid (BALF) fluid, cell subset population in lung‑draining lymph nodes (LNs), leukocytes infiltration and mucus production in the lungs of the animals was measured. Results demonstrated that intranasal administration of LRE significantly suppressed the level of inflammatory cell counts in BALF as well as populations of CD4+ T‑cells in lung draining LNs. Apart from that, LRE also significantly reduced the level of Th2 cytokines in BALF and IgE in the serum in OVA‑induced asthma. Histological analysis also demonstrated the amelioration of leukocytes infiltration and mucus production in the lungs. Overall, these findings demonstrated the attenuation of airway inflammation in the LRE‑treated mice therefore suggesting a promising alternative for the management of allergic airway inflammation.
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May-2019
Volume 17 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Muhamad SA, Muhammad NS, Ismail ND, Mohamud R, Safuan S and Nurul AA: Intranasal administration of Lignosus rhinocerotis (Cooke) Ryvarden (Tiger Milk mushroom) extract attenuates airway inflammation in murine model of allergic asthma. Exp Ther Med 17: 3867-3876, 2019
APA
Muhamad, S.A., Muhammad, N.S., Ismail, N.D., Mohamud, R., Safuan, S., & Nurul, A. (2019). Intranasal administration of Lignosus rhinocerotis (Cooke) Ryvarden (Tiger Milk mushroom) extract attenuates airway inflammation in murine model of allergic asthma. Experimental and Therapeutic Medicine, 17, 3867-3876. https://doi.org/10.3892/etm.2019.7416
MLA
Muhamad, S. A., Muhammad, N. S., Ismail, N. D., Mohamud, R., Safuan, S., Nurul, A."Intranasal administration of Lignosus rhinocerotis (Cooke) Ryvarden (Tiger Milk mushroom) extract attenuates airway inflammation in murine model of allergic asthma". Experimental and Therapeutic Medicine 17.5 (2019): 3867-3876.
Chicago
Muhamad, S. A., Muhammad, N. S., Ismail, N. D., Mohamud, R., Safuan, S., Nurul, A."Intranasal administration of Lignosus rhinocerotis (Cooke) Ryvarden (Tiger Milk mushroom) extract attenuates airway inflammation in murine model of allergic asthma". Experimental and Therapeutic Medicine 17, no. 5 (2019): 3867-3876. https://doi.org/10.3892/etm.2019.7416