Open Access

Nano hydroxyapatite induces glioma cell apoptosis by suppressing NF‑κB signaling pathway

  • Authors:
    • Guocai Guo
    • Ang Tian
    • Xiaolei Lan
    • Changqing Fu
    • Zhiyong Yan
    • Chao Wang
  • View Affiliations

  • Published online on: March 20, 2019     https://doi.org/10.3892/etm.2019.7418
  • Pages: 4080-4088
  • Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Nano‑sized hydroxyapatite (nHA) particles have been demonstrated to exert anti‑cancer effects on multiple cancer cell lines and animal models of cancer biology. However, the molecular mechanism underlying the effects of nHA particles on glioma cells remains unclear. The present study aimed to examine the effects of nHA on the behavior of glioma cells and investigate its underlying molecular mechanism. Rat glioma C6 cells and human glioma U87MG ATCC cells were exposed to nHA (20‑100 µg/ml), and its effects on cell morphology, viability, apoptosis, cell cycle, invasion and nuclear factor (NF)‑κB signaling were analyzed. Exposure of C6 and U87MG ATCC cells to 20 µg/ml nHA for 24 h caused cell detachment. Viability of C6 and U87MG ATCC cells were significantly reduced by nHA in a dose‑dependent manner (P<0.05). Nuclear staining with Hoechst 33258 exhibited clear chromatin condensation in C6 cells following 24 h exposure to ≥25 µg/ml nHA. Flow cytometry revealed that nHA (20‑100 µg/ml) significantly induced apoptosis and cell cycle G2/M arrest in C6 and U87MG ATCC cells (P<0.05). Transwell invasion assay demonstrated that nHA (20‑60 µg/ml) significantly inhibited invasion of U87MG ATCC cells (P<0.05). Furthermore, western blotting and confocal immunofluorescence microscopy revealed that nHA (20‑100 µg/ml) decreased NF‑κB p65 protein expression and blocked NF‑κB p65 nuclear translocation in C6 cells. The protein expression of NF‑κB target molecules, such as B cell lymphoma 2, cyclooxygenase‑2 and survivin, were also significantly reduced by nHA in a dose‑dependent manner in both C6 and U87MG ATCC cells (P<0.05). In conclusion, it was demonstrated that the inhibitory effect of nHA on glioma cells is likely associated with the downregulation of NF‑κB signaling.
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May-2019
Volume 17 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
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Spandidos Publications style
Guo G, Tian A, Lan X, Fu C, Yan Z and Wang C: Nano hydroxyapatite induces glioma cell apoptosis by suppressing NF‑κB signaling pathway. Exp Ther Med 17: 4080-4088, 2019.
APA
Guo, G., Tian, A., Lan, X., Fu, C., Yan, Z., & Wang, C. (2019). Nano hydroxyapatite induces glioma cell apoptosis by suppressing NF‑κB signaling pathway. Experimental and Therapeutic Medicine, 17, 4080-4088. https://doi.org/10.3892/etm.2019.7418
MLA
Guo, G., Tian, A., Lan, X., Fu, C., Yan, Z., Wang, C."Nano hydroxyapatite induces glioma cell apoptosis by suppressing NF‑κB signaling pathway". Experimental and Therapeutic Medicine 17.5 (2019): 4080-4088.
Chicago
Guo, G., Tian, A., Lan, X., Fu, C., Yan, Z., Wang, C."Nano hydroxyapatite induces glioma cell apoptosis by suppressing NF‑κB signaling pathway". Experimental and Therapeutic Medicine 17, no. 5 (2019): 4080-4088. https://doi.org/10.3892/etm.2019.7418