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Article

LncRNA CCAT1 negatively regulates miR‑181a‑5p to promote endometrial carcinoma cell proliferation and migration

  • Authors:
    • Jing Yu
    • Lijuan Jiang
    • Yutao Gao
    • Qijian Sun
    • Beibei Liu
    • Yong Hu
    • Xuesong Han
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology, The Third Affiliated Hospital of Kunming Medical University and Cancer Hospital of Yunnan Province, Kunming, Yunnan 650118, P.R. China, Department of Scientific Research and Education, The First Affiliated Hospital of Yunnan University of Traditional Chinese Medicine, Kunming, Yunnan 650200, P.R. China, Department of Gynecology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, P.R. China
  • Pages: 4259-4266
    |
    Published online on: March 20, 2019
       https://doi.org/10.3892/etm.2019.7422
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Abstract

Long non‑coding RNAs (lncRNAs) are identified as vital modulators in a number of biological processes, including tumorigenesis. However, the role of lncRNAs in endometrial carcinoma (EC) remains unknown. In the current study, the expression patterns, biological roles and functional mechanism of the lncRNA colon cancer associated transcript 1 (CCAT1) was examined in EC. The expression level of CCAT1 was significantly upregulated in EC tissue samples compared with matched adjacent healthy tissue samples from patients with endometrial cancer. Similarly, CCAT1 was significantly upregulated in several EC cell lines (KLE, Ishiwaka and HEC‑1‑A), compared with the normal human endometrial stromal cell line T‑HESC. Cell counting kit‑8 and Transwell migration assays demonstrated that CCAT1 knockdown significantly decreased EC cell proliferation and migration. In addition, CCAT1 was confirmed as a target gene of miR‑181a‑5p in EC. Overexpression of miR‑181a‑5p significantly decreased CCAT1 expression in EC cells, whilst knockdown of CCAT1 significantly increased miR‑181a‑5p expression in EC cells. Furthermore, miR‑181a‑5p expression was significantly downregulated in EC tissue samples compared with matched adjacent healthy tissue samples from patients with endometrial cancer. Similarly, miR‑181a‑5p expression was significantly downregulated in several EC cell lines (KLE, Ishiwaka and HEC‑1‑A), compared with normal human endometrial stromal cell line T‑HESC. In addition, rescue experiments demonstrated that inhibition of miR‑181a‑5p significantly reversed the effect of CCAT1 knockdown on EC cell proliferation and migration. The results suggest that CCAT1 promotes EC progression by acting as a molecular sponge of miR‑181a‑5p.
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Copy and paste a formatted citation
Spandidos Publications style
Yu J, Jiang L, Gao Y, Sun Q, Liu B, Hu Y and Han X: LncRNA CCAT1 negatively regulates miR‑181a‑5p to promote endometrial carcinoma cell proliferation and migration. Exp Ther Med 17: 4259-4266, 2019.
APA
Yu, J., Jiang, L., Gao, Y., Sun, Q., Liu, B., Hu, Y., & Han, X. (2019). LncRNA CCAT1 negatively regulates miR‑181a‑5p to promote endometrial carcinoma cell proliferation and migration. Experimental and Therapeutic Medicine, 17, 4259-4266. https://doi.org/10.3892/etm.2019.7422
MLA
Yu, J., Jiang, L., Gao, Y., Sun, Q., Liu, B., Hu, Y., Han, X."LncRNA CCAT1 negatively regulates miR‑181a‑5p to promote endometrial carcinoma cell proliferation and migration". Experimental and Therapeutic Medicine 17.5 (2019): 4259-4266.
Chicago
Yu, J., Jiang, L., Gao, Y., Sun, Q., Liu, B., Hu, Y., Han, X."LncRNA CCAT1 negatively regulates miR‑181a‑5p to promote endometrial carcinoma cell proliferation and migration". Experimental and Therapeutic Medicine 17, no. 5 (2019): 4259-4266. https://doi.org/10.3892/etm.2019.7422
Copy and paste a formatted citation
x
Spandidos Publications style
Yu J, Jiang L, Gao Y, Sun Q, Liu B, Hu Y and Han X: LncRNA CCAT1 negatively regulates miR‑181a‑5p to promote endometrial carcinoma cell proliferation and migration. Exp Ther Med 17: 4259-4266, 2019.
APA
Yu, J., Jiang, L., Gao, Y., Sun, Q., Liu, B., Hu, Y., & Han, X. (2019). LncRNA CCAT1 negatively regulates miR‑181a‑5p to promote endometrial carcinoma cell proliferation and migration. Experimental and Therapeutic Medicine, 17, 4259-4266. https://doi.org/10.3892/etm.2019.7422
MLA
Yu, J., Jiang, L., Gao, Y., Sun, Q., Liu, B., Hu, Y., Han, X."LncRNA CCAT1 negatively regulates miR‑181a‑5p to promote endometrial carcinoma cell proliferation and migration". Experimental and Therapeutic Medicine 17.5 (2019): 4259-4266.
Chicago
Yu, J., Jiang, L., Gao, Y., Sun, Q., Liu, B., Hu, Y., Han, X."LncRNA CCAT1 negatively regulates miR‑181a‑5p to promote endometrial carcinoma cell proliferation and migration". Experimental and Therapeutic Medicine 17, no. 5 (2019): 4259-4266. https://doi.org/10.3892/etm.2019.7422
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