Novel microRNA, miR‑sc6, modulates Schwann cell phenotype via targeting ErbB4

Chen,Chu; Liu,Qianyan; Hua,Hao; Wang,Xinghui; Wang,Pan; Cui,Zhiming; Qian,Tianmei

doi:10.3892/etm.2019.7426

Novel microRNA, miR‑sc6, modulates Schwann cell phenotype via targeting ErbB4

Authors:
- Chu Chen
- Qianyan Liu
- Hao Hua
- Xinghui Wang
- Pan Wang
- Zhiming Cui
- Tianmei Qian
View Affiliations

Affiliations: Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co‑innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu 226001, P.R. China, Department of Medicine, Xinglin College, Nantong University, Nantong, Jiangsu 226001, P.R. China, Department of Spine Surgery, The Second Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China
Published online on: March 20, 2019 https://doi.org/10.3892/etm.2019.7426
Pages: 4116-4122
Copyright : © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

MicroRNAs (miRNAs) are non‑coding RNAs that regulate various tissues and organs, including the nervous system. Peripheral nerve injury is a common pathology of the nervous system and leads to differential expressions of a variety of miRNAs. Previously, a group of novel miRNAs have been identified in rat proximal nerve segments after sciatic nerve transection. However, the biological functions of these novel miRNAs remain undetermined. The aim of the current study was therefore to identify the function of a novel miRNA, miR‑sc6, following nerve injury. Its target genes and effects on phenotypic modulation of Schwann cells were determined using a miR‑sc6 mimic transfection. These observations contribute to the understanding of miRNA involvement in peripheral nerve injury and the cognition of regulatory mechanisms in peripheral nerve regeneration.

View Figures

View References

1	O'Driscoll L: The emerging world of microRNAs. Anticancer Res. 26:4271–4278. 2006.PubMed/NCBI
2	Bartel DP: MicroRNAs: Target recognition and regulatory functions. Cell. 136:215–233. 2009. View Article : Google Scholar : PubMed/NCBI
3	Didiano D and Hobert O: Molecular architecture of a miRNA- regulated 3′UTR. RNA. 14:1297–1317. 2008. View Article : Google Scholar : PubMed/NCBI
4	Ambros V: The functions of animal microRNAs. Nature. 431:350–355. 2004. View Article : Google Scholar : PubMed/NCBI
5	Williams AE: Functional aspects of animal microRNAs. Cell Mol Life Sci. 65:545–562. 2008. View Article : Google Scholar : PubMed/NCBI
6	Shivdasani RA: MicroRNAs: Regulators of gene expression and cell differentiation. Blood. 108:3646–3653. 2006. View Article : Google Scholar : PubMed/NCBI
7	Erson AE and Petty EM: MicroRNAs in development and disease. Clin Genet. 74:296–306. 2008. View Article : Google Scholar : PubMed/NCBI
8	Zhang C: MicroRNAs: Role in cardiovascular biology and disease. Clin Sci (Lond). 114:699–706. 2008. View Article : Google Scholar : PubMed/NCBI
9	Erson AE and Petty EM: miRNAs and cancer: New research developments and potential clinical applications. Cancer Biol Ther. 8:2317–2322. 2009. View Article : Google Scholar : PubMed/NCBI
10	Lynam-Lennon N, Maher SG and Reynolds JV: The roles of microRNA in cancer and apoptosis. Biol Rev Camb Philos Soc. 84:55–71. 2009. View Article : Google Scholar : PubMed/NCBI
11	Pauley KM and Chan EK: MicroRNAs and their emerging roles in immunology. Ann N Y Acad Sci. 1143:226–239. 2008. View Article : Google Scholar : PubMed/NCBI
12	Yu B, Zhou S, Yi S and Gu X: The regulatory roles of non-coding RNAs in nerve injury and regeneration. Prog Neurobiol. 134:122–139. 2015. View Article : Google Scholar : PubMed/NCBI
13	Wu D and Murashov AK: Molecular mechanisms of peripheral nerve regeneration: Emerging roles of microRNAs. Front Physiol. 4:552013. View Article : Google Scholar : PubMed/NCBI
14	Gu X, Ding F, Yang Y and Liu J: Construction of tissue engineered nerve grafts and their application in peripheral nerve regeneration. Prog Neurobiol. 93:204–230. 2011. View Article : Google Scholar : PubMed/NCBI
15	Bosse F: Extrinsic cellular and molecular mediators of peripheral axonal regeneration. Cell Tissue Res. 349:5–14. 2012. View Article : Google Scholar : PubMed/NCBI
16	Bhatheja K and Field J: Schwann cells: Origins and role in axonal maintenance and regeneration. Int J Biochem Cell Biol. 38:1995–1999. 2006. View Article : Google Scholar : PubMed/NCBI
17	Bunge RP: Expanding roles for the Schwann cell: Ensheathment, myelination, trophism and regeneration. Curr Opin Neurobiol. 3:805–809. 1993. View Article : Google Scholar : PubMed/NCBI
18	Campbell WW: Evaluation and management of peripheral nerve injury. Clin Neurophysiol. 119:1951–1965. 2008. View Article : Google Scholar : PubMed/NCBI
19	Yu B, Zhou S, Wang Y, Ding G, Ding F and Gu X: Profile of microRNAs following rat sciatic nerve injury by deep sequencing: Implication for mechanisms of nerve regeneration. PLoS One. 6:e246122011. View Article : Google Scholar : PubMed/NCBI
20	Phay M, Kim HH and Yoo S: Dynamic change and target prediction of axon-specific microRNAs in regenerating sciatic nerve. PLoS One. 10:e01374612015. View Article : Google Scholar : PubMed/NCBI
21	Yi S, Wang QH, Zhao LL, Qin J, Wang YX, Yu B and Zhou SL: miR-30c promotes Schwann cell remyelination following peripheral nerve injury. Neural Regen Res. 12:1708–1715. 2017. View Article : Google Scholar : PubMed/NCBI
22	Yi S, Yuan Y, Chen Q, Wang X, Gong L, Liu J, Gu X and Li S: Regulation of Schwann cell proliferation and migration by miR-1 targeting brain-derived neurotrophic factor after peripheral nerve injury. Sci Rep. 6:291212016. View Article : Google Scholar : PubMed/NCBI
23	Li S, Zhang R, Yuan Y, Yi S, Chen Q, Gong L, Liu J, Ding F, Cao Z and Gu X: MiR-340 regulates fibrinolysis and axon regrowth following sciatic nerve injury. Mol Neurobiol. 54:4379–4389. 2017. View Article : Google Scholar : PubMed/NCBI
24	Li S, Yu B, Wang Y, Yao D, Zhang Z and Gu X: Identification and functional annotation of novel microRNAs in the proximal sciatic nerve after sciatic nerve transection. Sci China Life Sci. 54:806–812. 2011. View Article : Google Scholar : PubMed/NCBI
25	Yu B, Qian T, Wang Y, Zhou S, Ding G, Ding F and Gu X: miR-182 inhibits Schwann cell proliferation and migration by targeting FGF9 and NTM, respectively at an early stage following sciatic nerve injury. Nucleic Acids Res. 40:10356–10365. 2012. View Article : Google Scholar : PubMed/NCBI
26	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
27	Huang DW, Sherman BT, Tan Q, Kir J, Liu D, Bryant D, Guo Y, Stephens R, Baseler MW, Lane HC and Lempicki RA: DAVID bioinformatics resources: Expanded annotation database and novel algorithms to better extract biology from large gene lists. Nucleic Acids Res. 35:(Web Server Issue). W169–W175. 2007. View Article : Google Scholar : PubMed/NCBI
28	Rehmsmeier M, Steffen P, Hochsmann M and Giegerich R: Fast and effective prediction of microRNA/target duplexes. RNA. 10:1507–1517. 2004. View Article : Google Scholar : PubMed/NCBI
29	Palomo Irigoyen M, Tamayo Caro M, Pérez Andrés E, Barreira Manrique A, Varela Rey M and Woodhoo A: Isolation and purification of primary rodent schwann cells. Methods Mol Biol. 1791:81–93. 2018. View Article : Google Scholar : PubMed/NCBI
30	Liu B, Li J and Cairns MJ: Identifying miRNAs, targets and functions. Brief Bioinform. 15:1–19. 2014. View Article : Google Scholar : PubMed/NCBI
31	Li S, Liu Q, Wang Y, Gu Y, Liu D, Wang C, Ding G, Chen J, Liu J and Gu X: Differential gene expression profiling and biological process analysis in proximal nerve segments after sciatic nerve transection. PLoS One. 8:e570002013. View Article : Google Scholar : PubMed/NCBI
32	Gordon T: Nerve regeneration: Understanding biology and its influence on return of function after nerve transfers. Hand Clin. 32:103–117. 2016. View Article : Google Scholar : PubMed/NCBI
33	Gordon T, Sulaiman O and Boyd JG: Experimental strategies to promote functional recovery after peripheral nerve injuries. J Peripher Nerv Syst. 8:236–250. 2003. View Article : Google Scholar : PubMed/NCBI
34	Boyd JG and Gordon T: Glial cell line-derived neurotrophic factor and brain-derived neurotrophic factor sustain the axonal regeneration of chronically axotomized motoneurons in vivo. Exp Neurol. 183:610–619. 2003. View Article : Google Scholar : PubMed/NCBI
35	Oh SH, Kang JG, Kim TH, Namgung U, Song KS, Jeon BH and Lee JH: Enhanced peripheral nerve regeneration through asymmetrically porous nerve guide conduit with nerve growth factor gradient. J Biomed Mater Res A. 106:52–64. 2018. View Article : Google Scholar : PubMed/NCBI
36	Gonzalez-Perez F, Hernandez J, Heimann C, Phillips JB, Udina E and Navarro X: Schwann cells and mesenchymal stem cells in laminin- or fibronectin-aligned matrices and regeneration across a critical size defect of 15 mm in the rat sciatic nerve. J Neurosurg Spine. 28:109–118. 2018. View Article : Google Scholar : PubMed/NCBI
37	Yi S, Tang X, Yu J, Liu J, Ding F and Gu X: Microarray and qPCR analyses of wallerian degeneration in rat sciatic nerves. Front Cell Neurosci. 11:222017. View Article : Google Scholar : PubMed/NCBI
38	Gaudet AD, Popovich PG and Ramer MS: Wallerian degeneration: Gaining perspective on inflammatory events after peripheral nerve injury. J Neuroinflammation. 8:1102011. View Article : Google Scholar : PubMed/NCBI
39	Wang P, He J, Wang S, Wang X, Liu Q, Peng W and Qian T: miR-3075 inhibited the migration of Schwann cells by targeting Cntn2. Neurochem Res. 43:1879–1886. 2018. View Article : Google Scholar : PubMed/NCBI
40	Qian T, Wang X, Wang Y, Wang P, Liu Q, Liu J and Yi S: Novel miR-sc4 regulates the proliferation and migration of Schwann cells by targeting Cdk5r1. Mol Cell Biochem. 447:209–215. 2018. View Article : Google Scholar : PubMed/NCBI
41	Yi S, Zhang H, Gong L, Wu J, Zha G, Zhou S, Gu X and Yu B: Deep sequencing and bioinformatic analysis of lesioned sciatic nerves after crush injury. PLoS One. 10:e01434912015. View Article : Google Scholar : PubMed/NCBI
42	Gu Y, Chen C, Yi S, Wang S, Gong L, Liu J, Gu X, Zhao Q and Li S: miR-sc8 inhibits Schwann cell proliferation and migration by targeting Egfr. PLoS One. 10:e01451852015. View Article : Google Scholar : PubMed/NCBI
43	Yi S, Wang S, Zhao Q, Yao C, Gu Y, Liu J, Gu X and Li S: miR-sc3, a novel MicroRNA, promotes Schwann cell proliferation and migration by targeting Astn1. Cell Transplant. 25:973–982. 2016. View Article : Google Scholar : PubMed/NCBI
44	Stonecypher MS, Byer SJ, Grizzle WE and Carroll SL: Activation of the neuregulin-1/ErbB signaling pathway promotes the proliferation of neoplastic Schwann cells in human malignant peripheral nerve sheath tumors. Oncogene. 24:5589–5605. 2005. View Article : Google Scholar : PubMed/NCBI
45	Stonecypher MS, Chaudhury AR, Byer SJ and Carroll SL: Neuregulin growth factors and their ErbB receptors form a potential signaling network for schwannoma tumorigenesis. J Neuropathol Exp Neurol. 65:162–175. 2006. View Article : Google Scholar : PubMed/NCBI
46	Audisio C, Nicolino S, Scevola A, Tos P, Geuna S, Battiston B and Perroteau I: ErbB receptors modulation in different types of peripheral nerve regeneration. Neuroreport. 19:1605–1609. 2008. View Article : Google Scholar : PubMed/NCBI
47	Lyons DA, Pogoda HM, Voas MG, Woods IG, Diamond B, Nix R, Arana N, Jacobs J and Talbot WS: erbb3 and erbb2 are essential for schwann cell migration and myelination in zebrafish. Curr Biol. 15:513–524. 2005. View Article : Google Scholar : PubMed/NCBI
48	Atanasoski S, Scherer SS, Sirkowski E, Leone D, Garratt AN, Birchmeier C and Suter U: ErbB2 signaling in Schwann cells is mostly dispensable for maintenance of myelinated peripheral nerves and proliferation of adult Schwann cells after injury. J Neurosci. 26:2124–2131. 2006. View Article : Google Scholar : PubMed/NCBI
49	Chen S, Rio C, Ji RR, Dikkes P, Coggeshall RE, Woolf CJ and Corfas G: Disruption of ErbB receptor signaling in adult non-myelinating Schwann cells causes progressive sensory loss. Nat Neurosci. 6:1186–1193. 2003. View Article : Google Scholar : PubMed/NCBI