Open Access

MicroRNA‑193b acts as a tumor suppressor in colon cancer progression via targeting RAB22A

  • Authors:
    • Zhiming Fang
    • Chengren Li
    • Shouchao Li
  • View Affiliations

  • Published online on: March 26, 2019     https://doi.org/10.3892/etm.2019.7435
  • Pages: 3921-3928
  • Copyright: © Fang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

To explore microRNA (miR)‑193b expression and its potential role in colon cancer, reverse transcription‑​quantitative polymerase chain reaction was performed to detect the miR‑193b expression levels in 62 colon cancer tissues and normal adjacent tissues. The miR‑193b‑overexpressed cell line SW620 was used to study the role of miR‑193b in colon cancer. Subsequently, a Transwell assay and cell cycle assay were performed to observe the functional cell changes in the in vitro expression levels of miR‑193b. Results indicated that miR‑193b expression levels were significantly decreased in colon cancer tissues compared with adjacent normal tissue (P<0.001) and the expression of miR‑193b was significantly correlated with TNM staging (P=0.03) and lymph node invasion (P=0.007). Furthermore, overexpression of miR‑193b significantly decreased colon cancer cell cycle progression and its migration ability. In addition, the present findings suggested that the increased expression of miR‑193b by RAB22A, inhibited downstream proteins involved in the Ras signaling pathway, including the Ras and extracellular signal‑related kinase which may inhibit cancer proliferation and migration. In conclusion, the aim was to clarify the association of miR‑193b expression with colon cancer, and to explore the mechanism of miR‑193b in colon cancer proliferation and cell migration. The preliminary findings revealed that miR‑193b may have an important role in the process in colon cancer cell cycle and migration by the RAB22A‑Ras signaling pathway, thus providing a theoretical basis for miR‑193b as a potential molecular target for colon cancer treatment.
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May-2019
Volume 17 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
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Spandidos Publications style
Fang Z, Li C and Li S: MicroRNA‑193b acts as a tumor suppressor in colon cancer progression via targeting RAB22A. Exp Ther Med 17: 3921-3928, 2019
APA
Fang, Z., Li, C., & Li, S. (2019). MicroRNA‑193b acts as a tumor suppressor in colon cancer progression via targeting RAB22A. Experimental and Therapeutic Medicine, 17, 3921-3928. https://doi.org/10.3892/etm.2019.7435
MLA
Fang, Z., Li, C., Li, S."MicroRNA‑193b acts as a tumor suppressor in colon cancer progression via targeting RAB22A". Experimental and Therapeutic Medicine 17.5 (2019): 3921-3928.
Chicago
Fang, Z., Li, C., Li, S."MicroRNA‑193b acts as a tumor suppressor in colon cancer progression via targeting RAB22A". Experimental and Therapeutic Medicine 17, no. 5 (2019): 3921-3928. https://doi.org/10.3892/etm.2019.7435