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BMP4 and Neuregulin regulate the direction of mouse neural crest cell differentiation

  • Authors:
    • Shunqin Zhu
    • Wanhong Liu
    • Han‑Fei Ding
    • Hongjuan Cui
    • Liqun Yang
  • View Affiliations / Copyright

    Affiliations: School of Life Sciences, Southwest University, Chongqing 400715, P.R. China, School of Chemistry and Chemical Engineering, Chongqing University of Science and Technology, Chongqing 401331, P.R. China, Cancer Center, Medical College of Georgia, Augusta University, Augusta, GA 30912, USA, State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, P.R. China
    Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3883-3890
    |
    Published online on: March 26, 2019
       https://doi.org/10.3892/etm.2019.7439
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Abstract

The neural crest is a transient embryonic tissue that initially generates neural crest stem cells, which then migrate throughout the body to give rise to a variety of mature tissues. It was proposed that the fate of neural crest cells is gradually determined via environmental cues from the surrounding tissues. In the present study, neural crest cells were isolated and identified from mouse embryos. Bone morphogenetic protein 4 (BMP4) and Neuregulin (NRG) were employed to induce the differentiation of neural crest cells. Treatment with BMP4 revealed neuron‑associated differentiation; cells treated with NRG exhibited differentiation into the Schwann cell lineage, a type of glia. Soft agar clonogenic and neurosphere formation assays were conducted to investigate the effects of N‑Myc (MYCN) overexpression in neural crest cells; the number of colonies and neurospheres notably increased after 14 days. These findings demonstrated that the direction of cell differentiation may be affected by altering the factors present in the surrounding environment. In addition, MYCN may serve a key role in regulating neural crest cell differentiation.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu S, Liu W, Ding HF, Cui H and Yang L: BMP4 and Neuregulin regulate the direction of mouse neural crest cell differentiation. Exp Ther Med 17: 3883-3890, 2019.
APA
Zhu, S., Liu, W., Ding, H., Cui, H., & Yang, L. (2019). BMP4 and Neuregulin regulate the direction of mouse neural crest cell differentiation. Experimental and Therapeutic Medicine, 17, 3883-3890. https://doi.org/10.3892/etm.2019.7439
MLA
Zhu, S., Liu, W., Ding, H., Cui, H., Yang, L."BMP4 and Neuregulin regulate the direction of mouse neural crest cell differentiation". Experimental and Therapeutic Medicine 17.5 (2019): 3883-3890.
Chicago
Zhu, S., Liu, W., Ding, H., Cui, H., Yang, L."BMP4 and Neuregulin regulate the direction of mouse neural crest cell differentiation". Experimental and Therapeutic Medicine 17, no. 5 (2019): 3883-3890. https://doi.org/10.3892/etm.2019.7439
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu S, Liu W, Ding HF, Cui H and Yang L: BMP4 and Neuregulin regulate the direction of mouse neural crest cell differentiation. Exp Ther Med 17: 3883-3890, 2019.
APA
Zhu, S., Liu, W., Ding, H., Cui, H., & Yang, L. (2019). BMP4 and Neuregulin regulate the direction of mouse neural crest cell differentiation. Experimental and Therapeutic Medicine, 17, 3883-3890. https://doi.org/10.3892/etm.2019.7439
MLA
Zhu, S., Liu, W., Ding, H., Cui, H., Yang, L."BMP4 and Neuregulin regulate the direction of mouse neural crest cell differentiation". Experimental and Therapeutic Medicine 17.5 (2019): 3883-3890.
Chicago
Zhu, S., Liu, W., Ding, H., Cui, H., Yang, L."BMP4 and Neuregulin regulate the direction of mouse neural crest cell differentiation". Experimental and Therapeutic Medicine 17, no. 5 (2019): 3883-3890. https://doi.org/10.3892/etm.2019.7439
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