Open Access

BMP4 and Neuregulin regulate the direction of mouse neural crest cell differentiation

  • Authors:
    • Shunqin Zhu
    • Wanhong Liu
    • Han‑Fei Ding
    • Hongjuan Cui
    • Liqun Yang
  • View Affiliations

  • Published online on: March 26, 2019     https://doi.org/10.3892/etm.2019.7439
  • Pages: 3883-3890
  • Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The neural crest is a transient embryonic tissue that initially generates neural crest stem cells, which then migrate throughout the body to give rise to a variety of mature tissues. It was proposed that the fate of neural crest cells is gradually determined via environmental cues from the surrounding tissues. In the present study, neural crest cells were isolated and identified from mouse embryos. Bone morphogenetic protein 4 (BMP4) and Neuregulin (NRG) were employed to induce the differentiation of neural crest cells. Treatment with BMP4 revealed neuron‑associated differentiation; cells treated with NRG exhibited differentiation into the Schwann cell lineage, a type of glia. Soft agar clonogenic and neurosphere formation assays were conducted to investigate the effects of N‑Myc (MYCN) overexpression in neural crest cells; the number of colonies and neurospheres notably increased after 14 days. These findings demonstrated that the direction of cell differentiation may be affected by altering the factors present in the surrounding environment. In addition, MYCN may serve a key role in regulating neural crest cell differentiation.
View Figures
View References

Related Articles

Journal Cover

May-2019
Volume 17 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhu S, Liu W, Ding HF, Cui H and Yang L: BMP4 and Neuregulin regulate the direction of mouse neural crest cell differentiation. Exp Ther Med 17: 3883-3890, 2019
APA
Zhu, S., Liu, W., Ding, H., Cui, H., & Yang, L. (2019). BMP4 and Neuregulin regulate the direction of mouse neural crest cell differentiation. Experimental and Therapeutic Medicine, 17, 3883-3890. https://doi.org/10.3892/etm.2019.7439
MLA
Zhu, S., Liu, W., Ding, H., Cui, H., Yang, L."BMP4 and Neuregulin regulate the direction of mouse neural crest cell differentiation". Experimental and Therapeutic Medicine 17.5 (2019): 3883-3890.
Chicago
Zhu, S., Liu, W., Ding, H., Cui, H., Yang, L."BMP4 and Neuregulin regulate the direction of mouse neural crest cell differentiation". Experimental and Therapeutic Medicine 17, no. 5 (2019): 3883-3890. https://doi.org/10.3892/etm.2019.7439