H2S attenuates sepsis‑induced cardiac dysfunction via a PI3K/Akt‑dependent mechanism

Liu,Jianping; Li,Jianhua; Tian,Peigang; Guli,Bahaer; Weng,Guopeng; Li,Lei; Cheng,Qinghong

doi:10.3892/etm.2019.7440

H2S attenuates sepsis‑induced cardiac dysfunction via a PI3K/Akt‑dependent mechanism

Authors:
- Jianping Liu
- Jianhua Li
- Peigang Tian
- Bahaer Guli
- Guopeng Weng
- Lei Li
- Qinghong Cheng
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Affiliations: Department of Critical Care Medicine, Medical School of Shihezi University, Shihezi, Xinjiang 832008, P.R. China, Department of Critical Care Medicine, The First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang 832008, P.R. China
Published online on: March 26, 2019 https://doi.org/10.3892/etm.2019.7440
Pages: 4064-4072
Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The heart is the most vulnerable target organ in sepsis, and it has been previously reported that hydrogen sulfide (H2S) has a protective role in heart dysfunction caused by sepsis. Additionally, studies have demonstrated that the phosphatidylinositol‑3‑kinase (PI3K)/protein kinase B (Akt) signaling pathway has a protective function during sepsis. However, the potential association between H2S and PI3K/Akt in sepsis‑induced cardiac dysfunction is unclear. Therefore, the PI3K inhibitor LY294002 was used to investigate the role of PI3K/Akt signaling in the protective effects of H2S during sepsis‑induced myocardial injury. A rat sepsis model was established using cecal ligation and puncture (CLP) surgery. Sodium hydrosulfide, a H2S donor, was administered intraperitoneally (8.9 µmol/kg), and serum myocardial enzyme levels, inflammatory cytokine levels, cardiac histology and cardiomyocyte apoptosis were assessed to determine the extent of myocardial damage. The results demonstrated that exogenous H2S reduced serum myocardial enzyme levels, decreased the levels of the inflammatory factors tumor necrosis factor (TNF)‑α and interleukin (IL)‑6, and increased the level of anti‑inflammatory IL‑10 following CLP. Staining of histological sections demonstrated that myocardial damage and cardiomyocyte apoptosis were alleviated by the administration of exogenous H2S. Western blot analysis was used to detect phosphorylated and total PI3K and Akt levels, as well as NF‑κB, B‑cell lymphoma‑2, Bcl‑2‑associated X protein (Bax) and caspase levels, and the results demonstrated that H2S significantly increased PI3K and Akt phosphorylation. This indicated that the PI3K/Akt signaling pathway was activated by H2S. Additionally, H2S reduced Bax and caspase expression, indicating that apoptosis was inhibited, and decreased NF‑κB levels, indicating that inflammation was reduced. Furthermore, the PI3K inhibitor LY294002 eliminated the protective effects of H2S. In conclusion, the results of the current study suggest that exogenous H2S activates PI3K/Akt signaling to attenuate myocardial damage in sepsis.

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