Effect of white mange mixture in a murine model of psoriasis

Guo,Jiangtao; Liu,Jie

doi:10.3892/etm.2019.7641

Effect of white mange mixture in a murine model of psoriasis

Authors:
- Jiangtao Guo
- Jie Liu
View Affiliations

Affiliations: Pharmaceutical College, Guiyang University of Chinese Medicine, Guiyang, Guizhou 550025, P.R. China
Published online on: June 4, 2019 https://doi.org/10.3892/etm.2019.7641
Pages: 881-887

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Psoriasis is an autoimmune disease with periods of remission or aggravation. Until now, no effective treatment has been developed. The aim of this study was to assess the effect of the traditional Chinese medicine white mange mixture in a murine model of vaginal psoriasis. Female mice (n=70) were randomly divided into seven groups as follows: negative control group, positive control group, acitretin group, Xiaoying granule group, high-dose white mange mixture group, medium-dose white mange mixture group, and low-dose white mange mixture group. After vaginal psoriasis mouse model design, the inhibition of keratinocyte (KC) cell proliferating cell nuclear antigen (PCNA) was achieved by SP immunohistochemical method, spleen T lymphocyte apoptosis detection was assessed by using electron microscopy and granulocyte colony stimulating factor (GM‑CSF) levels were detected by ELISA method. According to our results, T lymphocyte nucleus appearance in the negative control group was normal whereas in all the doses of white mange mixture the nucleus significantly showed apoptotic trend. Compared with the negative control group, the amount of GM‑CSF in the serum of the model was significantly increased (P<0.01) while administration of white mange mixture in different doses decreased the GM‑CSF content significantly (P<0.01). White mange mixture can significantly inhibit vaginal psoriasis in a mouse model by decreasing the amount of epithelium KC cell PCNA and production of the inflammatory cytokines GM‑CSF in serum.

View Figures

View References

1	Nickoloff BJ and Nestle FO: Recent insights into the immunopathogenesis of psoriasis provide new therapeutic opportunities. J Clin Invest. 113:1664–1675. 2004. View Article : Google Scholar : PubMed/NCBI
2	Leonardi CL, Powers JL, Matheson RT, Goffe BS, Zitnik R, Wang A and Gottlieb AB; Etanercept Psoriasis Study Group, : Etanercept as monotherapy in patients with psoriasis. N Engl J Med. 349:2014–2022. 2003. View Article : Google Scholar : PubMed/NCBI
3	Menter A, Korman NJ, Elmets CA, Feldman SR, Gelfand JM, Gordon KB, Gottlieb AB, Koo JY, Lebwohl M, Lim HW, et al: Guidelines of care for the management of psoriasis and psoriatic arthritis: Section 4. Guidelines of care for the management and treatment of psoriasis with traditional systemic agents. J Am Acad Dermatol. 61:451–485. 2009. View Article : Google Scholar : PubMed/NCBI
4	Gudjonsson JE, Johnston A, Dyson M, Valdimarsson H and Elder JT: Mouse models of psoriasis. J Invest Dermatol. 127:1292–1308. 2007. View Article : Google Scholar : PubMed/NCBI
5	Ungureanu A, Zlatian O, Mitroi G, Drocaş A, Ţîrcă T, Călina D, Dehelean C, Docea AO, Izotov BN, Rakitskii VN, et al: Staphylococcus aureus colonisation in patients from a primary regional hospital. Mol Med Rep. 16:8771–8780. 2017. View Article : Google Scholar : PubMed/NCBI
6	Cargill M, Schrodi SJ, Chang M, Garcia VE, Brandon R, Callis KP, Matsunami N, Ardlie KG, Civello D, Catanese JJ, et al: A large-scale genetic association study confirms IL12B and leads to the identification of IL23R as psoriasis-risk genes. Am J Hum Genet. 80:273–290. 2007. View Article : Google Scholar : PubMed/NCBI
7	Krueger JG: The immunologic basis for the treatment of psoriasis with new biologic agents. J Am Acad Dermatol. 46:1–26. 2002. View Article : Google Scholar : PubMed/NCBI
8	Reich K, Nestle FO, Papp K, Ortonne JP, Evans R, Guzzo C, Li S, Dooley LT and Griffiths CE; EXPRESS study investigators, : Infliximab induction and maintenance therapy for moderate-to-severe psoriasis: A phase III, multicentre, double-blind trial. Lancet. 366:1367–1374. 2005. View Article : Google Scholar : PubMed/NCBI
9	Nomura I, Goleva E, Howell MD, Hamid QA, Ong PY, Hall CF, Darst MA, Gao B, Boguniewicz M, Travers JB, et al: Cytokine milieu of atopic dermatitis, as compared to psoriasis, skin prevents induction of innate immune response genes. J Immunol. 171:3262–3269. 2003. View Article : Google Scholar : PubMed/NCBI
10	Stern RS, Nijsten T, Feldman SR, Margolis DJ and Rolstad T: Psoriasis is common, carries a substantial burden even when not extensive, and is associated with widespread treatment dissatisfaction. J Investig Dermatol Symp Proc. 9:136–139. 2004. View Article : Google Scholar : PubMed/NCBI
11	Gottlieb AB, Evans R, Li S, Dooley LT, Guzzo CA, Baker D, Bala M, Marano CW and Menter A: Infliximab induction therapy for patients with severe plaque-type psoriasis: A randomized, double-blind, placebo-controlled trial. J Am Acad Dermatol. 51:534–542. 2004. View Article : Google Scholar : PubMed/NCBI
12	Sani TA, Mohammadpour E, Mohammadi A, Memariani T, Yazdi MV, Rezaee R, Calina D, Docea AO, Goumenou M, Etemad L, et al: Cytotoxic and apoptogenic properties of Dracocephalum kotschyi aerial part different fractions on calu-6 and mehr-80 lung cancer cell lines. Farmacia. 65:189–199. 2017.
13	Ho SG, Yeung CK and Chan HH: Methotrexate versus traditional Chinese medicine in psoriasis: A randomized, placebo-controlled trial to determine efficacy, safety and quality of life. Clin Exp Dermatol. 35:717–722. 2010. View Article : Google Scholar : PubMed/NCBI
14	Lu C, Deng J, Li L, Wang D and Li G: Application of metabolomics on diagnosis and treatment of patients with psoriasis in traditional Chinese medicine. Biochim Biophys Acta. 1844:280–288. 2014. View Article : Google Scholar : PubMed/NCBI
15	Geiger JM and Walker M: Is there a reproductive safety risk in male patients treated with acitretin (neotigason/soriatane?). Dermatology. 205:105–107. 2002. View Article : Google Scholar : PubMed/NCBI
16	Dogra S and Yadav S: Acitretin in psoriasis: An evolving scenario. Int J Dermatol. 53:525–538. 2014. View Article : Google Scholar : PubMed/NCBI
17	Yang L, Wu X and Ma J: Traditional Chinese medicine for the treatment of psoriasis vulgaris: A systematic review. Afr J Microbiol Res. 6:7040–7047. 2012.
18	Liu C, Bi Z, Zhu Y and Li P: Simultaneous determination of four kinds of bioactive components in radix scrophulariae by HPLC. Chung Kuo Yao Hsueh Tsa Chih. 42:1614–1616. 2007.(In Chinese).
19	Allan BF, Dutra HP, Goessling LS, Barnett K, Chase JM, Marquis RJ, Pang G, Storch GA, Thach RE and Orrock JL: Invasive honeysuckle eradication reduces tick-borne disease risk by altering host dynamics. Proc Natl Acad Sci USA. 107:18523–18527. 2010. View Article : Google Scholar : PubMed/NCBI
20	Stpiczyńska M, Nepi M and Zych M: Nectaries and male-biased nectar production in protandrous flowers of a perennial umbellifer Angelica sylvestris L. (Apiaceae). Plant Syst Evol. 301:1099–1113. 2015. View Article : Google Scholar
21	Zhang Y, Tu C, Zhang D, Zheng Y, Peng Z, Feng Y, Xiao S and Li Z: Wnt/β-catenin and Wnt5a/Ca pathways regulate proliferation and apoptosis of keratinocytes in psoriasis lesions. Cell Physiol Biochem. 36:1890–1902. 2015. View Article : Google Scholar : PubMed/NCBI
22	Kawahira K: Immunohistochemical staining of proliferating cell nuclear antigen (PCNA) in malignant and nonmalignant skin diseases. Arch Dermatol Res. 291:413–418. 1999. View Article : Google Scholar : PubMed/NCBI
23	Yonei T, Watanabe K, Sato T and Yamadori I: Induction of psoriasis by human recombinant granulocyte colony stimulating factor in a patient with small cell lung cancer. Nihon Kokyuki Gakkai Zasshi. 39:438–441. 2001.(In Japanese). PubMed/NCBI
24	Mössner R, Beckmann I, Hallermann C, Neumann C and Reich K: Granulocyte colony-stimulating-factor-induced psoriasiform dermatitis resembles psoriasis with regard to abnormal cytokine expression and epidermal activation. Exp Dermatol. 13:340–346. 2004. View Article : Google Scholar : PubMed/NCBI
25	Stoddart CA, Joshi P, Sloan B, Bare JC, Smith PC, Allaway GP, Wild CT and Martin DE: Potent activity of the HIV-1 maturation inhibitor bevirimat in SCID-hu Thy/Liv mice. PLoS One. 2:e12512007. View Article : Google Scholar : PubMed/NCBI
26	Yoshiji H, Kuriyama S, Yoshii J, Ikenaka Y, Noguchi R, Hicklin DJ, Wu Y, Yanase K, Namisaki T, Yamazaki M, et al: Vascular endothelial growth factor and receptor interaction is a prerequisite for murine hepatic fibrogenesis. Gut. 52:1347–1354. 2003. View Article : Google Scholar : PubMed/NCBI
27	Song XY, Sun LN, Zheng NN and Zhang HP: Effect of hyperbaric oxygen preconditioning on spleen lymphocytes and cell adhesion molecules after skin transplantation in mice. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 18:1275–1277. 2010.(In Chinese). PubMed/NCBI
28	Koynova R, Tarahovsky YS, Wang L and MacDonald RC: Lipoplex formulation of superior efficacy exhibits high surface activity and fusogenicity, and readily releases DNA. Biochim Biophys Acta. 1768:375–386. 2007. View Article : Google Scholar : PubMed/NCBI
29	Zheng H and Li X: Effect of white mange medicine mixture on mice models of psoriasis. Chin J Derm Venerol Integr Trad W Med. 11:149–151. 2012.
30	Kryczek I, Bruce AT, Gudjonsson JE, Johnston A, Aphale A, Vatan L, Szeliga W, Wang Y, Liu Y, Welling TH, et al: Induction of IL-17⁺ T cell trafficking and development by IFN-γ: Mechanism and pathological relevance in psoriasis. J Immunol. 181:4733–4741. 2008. View Article : Google Scholar : PubMed/NCBI
31	Zhang XJ, Huang W, Yang S, Sun LD, Zhang FY, Zhu QX, Zhang FR, Zhang C, Du WH, Pu XM, et al: Psoriasis genome-wide association study identifies susceptibility variants within LCE gene cluster at 1q21. Nat Genet. 41:205–210. 2009. View Article : Google Scholar : PubMed/NCBI
32	Tyring S, Gottlieb A, Papp K, Gordon K, Leonardi C, Wang A, Lalla D, Woolley M, Jahreis A, Zitnik R, et al: Etanercept and clinical outcomes, fatigue, and depression in psoriasis: Double-blind placebo-controlled randomised phase III trial. Lancet. 367:29–35. 2006. View Article : Google Scholar : PubMed/NCBI
33	Chiricozzi A, Guttman-Yassky E, Suárez-Fariñas M, Nograles KE, Tian S, Cardinale I, Chimenti S and Krueger JG: Integrative responses to IL-17 and TNF-α in human keratinocytes account for key inflammatory pathogenic circuits in psoriasis. J Invest Dermatol. 131:677–687. 2011. View Article : Google Scholar : PubMed/NCBI
34	Park JS, Rhyu JW, Kim CJ, Kim HS, Lee SY, Kwon YI, Namkoong SE, Sin HS and Um SJ: Neoplastic change of squamo-columnar junction in uterine cervix and vaginal epithelium by exogenous estrogen in hpv-18 URR E6/E7 transgenic mice. Gynecol Oncol. 89:360–368. 2003. View Article : Google Scholar : PubMed/NCBI
35	Zhang Q, Peng T and Shi H: Effects of BAIBI capsules on psoriatic mice and its mechanism of action. J Pract Dermatol. 10:234–239. 2017.(In Chinese).
36	Liu H, Li Q, Wen X and Ye J: Xiaoyin no. 2′ effect of content on the histopathology and the content of PCNA in psoriatic lesions of Guinea pig. J Yunnan Univ Tradit Chin Med. 38:13–16. 2015.(In Chinese).
37	Zhou Y and Yu R: Observation of combined acitretin capsule and Xiaoyin granule therapy for 60 patients with psoriasis vulgaris. Jilin Med J. 33:6504–6505. 2012.(In Chinese).
38	Nanjappa MK, Medrano TI, March AG and Cooke PS: Neonatal uterine and vaginal cell proliferation and adenogenesis are independent of estrogen receptor 1 (ESR1) in the mouse. Biol Reprod. 92:782015. View Article : Google Scholar : PubMed/NCBI
39	Zhou X, Krueger JG, Kao MC, Lee E, Du F, Menter A, Wong WH and Bowcock AM: Novel mechanisms of T-cell and dendritic cell activation revealed by profiling of psoriasis on the 63,100-element oligonucleotide array. Physiol Genomics. 13:69–78. 2003. View Article : Google Scholar : PubMed/NCBI
40	Lew W, Bowcock AM and Krueger JG: Psoriasis vulgaris: Cutaneous lymphoid tissue supports T-cell activation and ‘Type 1’ inflammatory gene expression. Trends Immunol. 25:295–305. 2004. View Article : Google Scholar : PubMed/NCBI
41	Laggner U, Di Meglio P, Perera GK, Hundhausen C, Lacy KE, Ali N, Smith CH, Hayday AC, Nickoloff BJ and Nestle FO: Identification of a novel proinflammatory human skin-homing Vγ9Vδ2 T cell subset with a potential role in psoriasis. J Immunol. 187:2783–2793. 2011. View Article : Google Scholar : PubMed/NCBI
42	Fujita H, Shemer A, Suárez-Fariñas M, Johnson-Huang LM, Tintle S, Cardinale I, Fuentes-Duculan J, Novitskaya I, Carucci JA, Krueger JG, et al: Lesional dendritic cells in patients with chronic atopic dermatitis and psoriasis exhibit parallel ability to activate T-cell subsets. J Allergy Clin Immunol. 128:574–582. 2011. View Article : Google Scholar : PubMed/NCBI
43	Yuan Q and Li X: Experimental study on regulating action of psoriasis model of guinea pig T cell activation by TCM psoriasis mixture. Clin J Chin Med. 6:46–49. 2014.(In Chinese).
44	Chen X, Lu Y and Li X: Effects of white mange mixture on Expression of CXCR2 protein of HaCaT cells and content of IL-6. Zhonghua Zhongyiyao Xuekan. 33:2710–2712. 2015.(In Chinese).
45	Xu W, Mao C, Feng R and Wang J: Correlation analysis of blood heat syndrome with psoriasis vulgaris and TH17 related cytokines. Guiding J Tradit Chin Med Pharm. 22:5–28. 2016.(In Chinese).
46	Kruglikov IL and Wollina U: The role of subcutaneous adipose tissue in psoriasis. J Biol Regul Homeost Agents. 32:159–161. 2018.PubMed/NCBI
47	Jobanputra P: Polyarteritis nodosa. Diagnostic challenges in a patient with cutaneous vasculitis, psoriasis, psoriatic arthritis and pancytopenia: Fatal progression after treatment with G-CSF. Oxf Med Case Rep. 2016:86–90. 2016. View Article : Google Scholar
48	Scholz T, Weigert A, Brüne B, Sadik CD, Böhm B and Burkhardt H: GM-CSF in murine psoriasiform dermatitis: Redundant and pathogenic roles uncovered by antibody-induced neutralization and genetic deficiency. PLoS One. 12:e01826462017. View Article : Google Scholar : PubMed/NCBI
49	Witte E, Kokolakis G, Witte K, Philipp S, Doecke WD, Babel N, Wittig BM, Warszawska K, Kurek A, Erdmann-Keding M, et al: IL-19 is a component of the pathogenetic IL-23/IL-17 cascade in psoriasis. J Invest Dermatol. 134:2757–2767. 2014. View Article : Google Scholar : PubMed/NCBI
50	Martin G, Guérard S, Fortin MM, Rusu D, Soucy J, Poubelle PE and Pouliot R: Pathological crosstalk in vitro between T lymphocytes and lesional keratinocytes in psoriasis: Necessity of direct cell-to-cell contact. Lab Invest. 92:1058–1070. 2012. View Article : Google Scholar : PubMed/NCBI
51	Yang C, Chen M and Tao C: Effect of baibi ointment to psoriasis guinea pig model. J Chengdu Univ TCM. 34:55–57. 2011.(In Chinese).
52	Shi XL, Pan YM, Ma HY and Yang XF: Treatment of psoriasis vulgaris by NB-UVB combined with traditional Chinese materia medica bath: A clinical observation. Chin J Laser Med Surg. 20:314–317. 2011.(In Chinese).
53	Buquicchio R, Foti C, Loconsole F, Polimeno L and Ventura MT: Clusterin serum level: How does it affect psoriatic patients? J Biol Regul Homeost Agents. 31:785–789. 2017.PubMed/NCBI
54	Cai Y, Zhang J, Yan Z, Zhang L, Yin J, Ren Y, Zhang G, Yin Z and Li H: Effect of Caishi Xiaoyin on serum levels of MCP-1, M-CSF and MIP-1 alpha in patients with psoriasis vulgaris. Chin J Clin Ration Drug Use. 8:107–108. 2015.(In Chinese).