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Article Open Access

Aberrant expression and mechanism of miR‑130b‑3p/phosphatase and tensin homolog in nephroblastoma in children

  • Authors:
    • Yifeng Hu
    • Jingtie Yan
  • View Affiliations / Copyright

    Affiliations: Department of Pediatric Surgery, Zaozhuang Municipal Hospital, Zaozhuang, Shandong 277102, P.R. China
    Copyright: © Hu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1021-1028
    |
    Published online on: June 4, 2019
       https://doi.org/10.3892/etm.2019.7643
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Abstract

Nephroblastoma is the most common renal tumor in children. Abnormal expression of microRNAs (miRs) has been reported to be involved in the progression of various types of cancers. However, the role and underlying mechanism of miR‑130b‑3p in nephroblastoma remains unknown. Therefore, the present study aimed to explore the role and possible mechanism of miR‑130b‑3p in nephroblastoma in children. The present study identified that miR‑130b‑3p was highly expressed in nephroblastoma tissues obtained from children with nephroblastoma. To better understand the functions and the molecular mechanisms of miR‑130b‑3p in nephroblastoma, TargetScan was used to identify the potential targets of miR‑130b‑3p. Phosphatase and tensin homolog (PTEN), was identified as a target gene of miR‑130b‑3p, and it was observed to be downregulated in nephroblastoma. Further analysis indicated that miR‑130b‑3p inhibitor could significantly reduce cell proliferation, induce apoptosis and suppress the Akt/nuclear factor‑κB/survivin signaling pathway in nephroblastoma cells. Notably, all these effects of miR‑130b‑3p on nephroblastoma cells were reversed by PTEN‑small interfering RNA. In summary, the present study suggested that the miR‑130b‑3p/PTEN axis could serve a critical role in the progression and development of nephroblastoma. It also suggests that miR‑130b‑3p might be a valuable clinical biomarker and therapeutic target for nephroblastoma in children.
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Copy and paste a formatted citation
Spandidos Publications style
Hu Y and Yan J: Aberrant expression and mechanism of miR‑130b‑3p/phosphatase and tensin homolog in nephroblastoma in children. Exp Ther Med 18: 1021-1028, 2019.
APA
Hu, Y., & Yan, J. (2019). Aberrant expression and mechanism of miR‑130b‑3p/phosphatase and tensin homolog in nephroblastoma in children. Experimental and Therapeutic Medicine, 18, 1021-1028. https://doi.org/10.3892/etm.2019.7643
MLA
Hu, Y., Yan, J."Aberrant expression and mechanism of miR‑130b‑3p/phosphatase and tensin homolog in nephroblastoma in children". Experimental and Therapeutic Medicine 18.2 (2019): 1021-1028.
Chicago
Hu, Y., Yan, J."Aberrant expression and mechanism of miR‑130b‑3p/phosphatase and tensin homolog in nephroblastoma in children". Experimental and Therapeutic Medicine 18, no. 2 (2019): 1021-1028. https://doi.org/10.3892/etm.2019.7643
Copy and paste a formatted citation
x
Spandidos Publications style
Hu Y and Yan J: Aberrant expression and mechanism of miR‑130b‑3p/phosphatase and tensin homolog in nephroblastoma in children. Exp Ther Med 18: 1021-1028, 2019.
APA
Hu, Y., & Yan, J. (2019). Aberrant expression and mechanism of miR‑130b‑3p/phosphatase and tensin homolog in nephroblastoma in children. Experimental and Therapeutic Medicine, 18, 1021-1028. https://doi.org/10.3892/etm.2019.7643
MLA
Hu, Y., Yan, J."Aberrant expression and mechanism of miR‑130b‑3p/phosphatase and tensin homolog in nephroblastoma in children". Experimental and Therapeutic Medicine 18.2 (2019): 1021-1028.
Chicago
Hu, Y., Yan, J."Aberrant expression and mechanism of miR‑130b‑3p/phosphatase and tensin homolog in nephroblastoma in children". Experimental and Therapeutic Medicine 18, no. 2 (2019): 1021-1028. https://doi.org/10.3892/etm.2019.7643
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