Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
August-2019 Volume 18 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2019 Volume 18 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Calcitonin gene‑related peptide enhances osteogenic differentiation and recruitment of bone marrow mesenchymal stem cells in rats

  • Authors:
    • Sen Jia
    • Shi‑Jian Zhang
    • Xu‑Dong Wang
    • Zi‑Hui Yang
    • Ya‑Nan Sun
    • Anand Gupta
    • Rui Hou
    • De‑Lin Lei
    • Kai‑Jin Hu
    • Wei‑Min Ye
    • Lei Wang
  • View Affiliations / Copyright

    Affiliations: Department of Oral and Maxillofacial Surgery, School of Stomatology Xi'an Medical University, Xi'an, Shaanxi 710032, P.R. China, Department of Oral and Maxillofacial‑Head and Neck Oncology, Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology, National Clinical Research Center of Stomatology, Shanghai 200011, P.R. China, State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, State Key Laboratory of Military Stomatology, Department of Pediatric Stomatology, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, Department of Oral and Maxillofacial Surgery, Government Medical College and Hospital, Chandigarh, Haryana 160030, India
    Copyright: © Jia et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1039-1046
    |
    Published online on: June 11, 2019
       https://doi.org/10.3892/etm.2019.7659
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The present study evaluated the effects of calcitonin gene‑related peptide (CGRP) on bone marrow mesenchymal stem cells (BMMSCs) in vitro and in a rat model of mandibular distraction osteogenesis (MDO). Rat BMMSCs were isolated then treated with CGRP or CGRP antagonist (CGRP8‑37). The proliferation and migration ability of BMMSCs was determined using 5‑bromo‑2'‑deoxyuridine and Transwell assays, respectively. Osteogenic‑related gene expression was analyzed with reverse transcription‑quantitative polymerase chain reaction. For the in vivo analysis, thirty MDO rats were randomly assigned to control, CGRP or CGRP8‑37 groups. To evaluate the mobilization of BMMSCs, nestin and stromal cell‑derived factor 1 (SDF‑1) were detected by immunohistochemistry and ELISA. Rats were sacrificed following 14 days and new bone formation was assessed by histological and micro‑computed tomography analysis. In the in vitro results, the CGRP group demonstrated significantly higher migration and proliferation, as well as enhanced alkaline phosphatase and runt‑related transcription factor 2 expression compared with the control. In the in vivo experiments, bone mineral density of the newly formed bone in the CGRP group was significantly higher than controls. The nestin and SDF‑1 expression in the CGRP group was also significantly upregulated. In conclusion, the present study demonstrated that CGRP administration increased new bone formation, possibly via enhancing BMMSC migration and differentiation in MDO rats.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

van Strijen PJ, Breuning KH, Becking AG, Perdijk FB and Tuinzing DB: Complications in bilateral mandibular distraction osteogenesis using internal devices. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 96:392–397. 2003. View Article : Google Scholar : PubMed/NCBI

2 

Garcia-Castellano JM, Diaz-Herrera P and Morcuende JA: Is bone a target-tissue for the nervous system? New advances on the understanding of their interactions. Iowa Orthop J. 20:49–58. 2000.PubMed/NCBI

3 

Suzuki A, Uemura T and Nakamura H: Control of bone remodeling by nervous system. Neural involvement in fracture healing and bone regeneration. Clin Calcium. 20:1820–1827. 2010.(In Japanese). PubMed/NCBI

4 

Anderson JJ, Woelffer KE, Holtzman JJ and Jacobs AM: Bisphosphonates for the treatment of Charcot neuroarthropathy J Foot Ankle Surg. 43:285–289. 2004. View Article : Google Scholar : PubMed/NCBI

5 

Du ZJ, Wang L, Lei DL, Liu BL, Cao J, Zhang P and Ma Q: Nerve growth factor injected systemically improves the recovery of the inferior alveolar nerve in a rabbit model of mandibular distraction osteogenesis. Br J Oral Maxillofac Surg. 49:557–561. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Wang L, Cao J, Lei DL, Cheng XB, Zhou HZ, Hou R, Zhao YH and Cui FZ: Application of nerve growth factor by gel increases formation of bone in mandibular distraction osteogenesis in rabbits. Br J Oral Maxillofac Surg. 48:515–519. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Wang L, Shi X, Zhao R, Halloran BP, Clark DJ, Jacobs CR and Kingery WS: Calcitonin-gene-related peptide stimulates stromal cell osteogenic differentiation and inhibits RANKL induced NF-kappaB activation, osteoclastogenesis and bone resorption. Bone. 46:1369–1379. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Hong HS, Lee J, Lee E, Kwon YS, Lee E, Ahn W, Jiang MH, Kim JC and Son Y: A new role of substance P as an injury-inducible messenger for mobilization of CD29(+) stromal-like cells. Nat Med. 15:425–435. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Yu X, Lv L, Zhang J, Zhang T, Xiao C and Li S: Expression of neuropeptides and bone remodeling-related factors during periodontal tissue regeneration in denervated rats. J Mol Histol. 46:195–203. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Sample SJ, Heaton CM, Behan M, Bleedorn JA, Racette MA, Hao Z and Muir P: Role of calcitonin gene-related peptide in functional adaptation of the skeleton. PLoS One. 9:e1139592014. View Article : Google Scholar : PubMed/NCBI

11 

Wimalawansa SJ: Calcitonin gene-related peptide, and its receptors: Molecular genetics, physiology, pathophysiology and therapeutic potentials. Endocr Rev. 17:533–585. 1996. View Article : Google Scholar : PubMed/NCBI

12 

Yang Z, Wu B, Jia S, Zhao Y, Hou R, Liu X, Wang X, Chen L, Yang X, Lei D and Wang L: The mechanically activated p38/MMP-2 signaling pathway promotes bone marrow mesenchymal stem cell migration in rats. Arch Oral Biol. 76:55–60. 2017. View Article : Google Scholar : PubMed/NCBI

13 

Yang ZH, Wu BL, Ye C, Jia S, Yang XJ, Hou R, Lei DL and Wang L: Targeting P38 pathway regulates bony formation via MSC recruitment during mandibular distraction osteogenesis in rats. Int J Med Sci. 13:783–789. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Wang L, Zhao Y, Liu Y, Akiyama K, Chen C, Qu C, Jin Y and Shi S: IFN-γ and TNF-α synergistically induce mesenchymal stem cell impairment and tumorigenesis via NFκB signaling. Stem Cells. 31:1383–1395. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Wang L, Liu S, Zhao Y, Liu D, Liu Y, Chen C, Karray S, Shi S and Jin Y: Osteoblast-induced osteoclast apoptosis by fas ligand/FAS pathway is required for maintenance of bone mass. Cell Death Differ. 22:1654–1664. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Wu B, Wang L, Yang X, Mao M, Ye C, Liu P, Yang Z, Yang X, Lei D and Zhang C: Norepinephrine inhibits mesenchymal stem cell chemotaxis migration by increasing stromal cell-derived factor-1 secretion by vascular endothelial cells via NE/abrd3/JNK pathway. Exp Cell Res. 349:214–220. 2016. View Article : Google Scholar : PubMed/NCBI

17 

Villa I, Melzi R, Pagani F, Ravasi F, Rubinacci A and Guidobono F: Effects of calcitonin gene-related peptide and amylin on human osteoblast-like cells proliferation. Eur J Pharmacol. 409:273–278. 2000. View Article : Google Scholar : PubMed/NCBI

18 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

19 

Wang T, Cao J, Du ZJ, Zhang YB, Liu YP, Wang L and Lei DL: Effects of sympathetic innervation loss on mandibular distraction osteogenesis. J Craniofac Surg. 23:1524–1528. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Zhang YB, Wang L, Jia S, Du ZJ, Zhao YH, Liu YP and Lei DL: Local injection of substance P increases bony formation during mandibular distraction osteogenesis in rats. Br J Oral Maxillofac Surg. 52:697–702. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Imai S, Tokunaga Y, Maeda T, Kikkawa M and Hukuda S: Calcitonin gene-related peptide, substance P and tyrosine hydroxylase-immunoreactive innervation of rat bone marrows: An immunohistochemical and ultrastructural investigation on possible efferent and afferent mechanisms. J Orthop Res. 15:133–140. 1997. View Article : Google Scholar : PubMed/NCBI

22 

Yoo YM, Kwag JH, Kim KH and Kim CH: Effects of neuropeptides and mechanical loading on bone cell resorption in vitro. Int J Mol Sci. 15:5874–5883. 2014. View Article : Google Scholar : PubMed/NCBI

23 

MacIntyre I: Amylinamide, bone conservation and pancreatic beta cells. Lancet. 2:1026–1027. 1989. View Article : Google Scholar : PubMed/NCBI

24 

Tippins JR, Morris HR, Panico M, Etienne T, Bevis P, Girgis S, MacIntyre I, Azria M and Attinger M: The myotropic and plasma-calcium modulating effects of calcitonin gene-related peptide (CGRP). Neuropeptides. 4:425–434. 1984. View Article : Google Scholar : PubMed/NCBI

25 

Cornish J, Callon KE, Lin CQ, Xiao CL, Gamble GD, Cooper GJ and Reid IR: Comparison of the effects of calcitonin gene-related peptide and amylin on osteoblasts. J Bone Miner Res. 14:1302–1309. 1999. View Article : Google Scholar : PubMed/NCBI

26 

Ballica R, Valentijn K, Khachatryan A, Guerder S, Kapadia S, Gundberg C, Gilligan J, Flavell RA and Vignery A: Targeted expression of calcitonin gene-related peptide to osteoblasts increases bone density in mice. J Bone Miner Res. 14:1067–1074. 1999. View Article : Google Scholar : PubMed/NCBI

27 

Xu G and Jiang D: The role and mechanism of exogenous calcitonin gene-related peptide on mesenchymal stem cell proliferation and osteogenetic formation. Cell Biochem Biophys. 69:369–378. 2014. View Article : Google Scholar : PubMed/NCBI

28 

Fang Z, Yang Q, Xiong W, Li GH, Liao H, Xiao J and Li F: Effect of CGRP-adenoviral vector transduction on the osteoblastic differentiation of rat adipose-derived stem cells. PLoS One. 8:e727382013. View Article : Google Scholar : PubMed/NCBI

29 

Nagao S, Goto T, Kataoka S, Toyono T, Joujima T, Egusa H, Yatani H, Kobayashi S and Maki K: Expression of neuropeptide receptor mRNA during osteoblastic differentiation of mouse iPS cells. Neuropeptides. 48:399–406. 2014. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Jia S, Zhang SJ, Wang XD, Yang ZH, Sun YN, Gupta A, Hou R, Lei DL, Hu KJ, Ye WM, Ye WM, et al: Calcitonin gene‑related peptide enhances osteogenic differentiation and recruitment of bone marrow mesenchymal stem cells in rats. Exp Ther Med 18: 1039-1046, 2019.
APA
Jia, S., Zhang, S., Wang, X., Yang, Z., Sun, Y., Gupta, A. ... Wang, L. (2019). Calcitonin gene‑related peptide enhances osteogenic differentiation and recruitment of bone marrow mesenchymal stem cells in rats. Experimental and Therapeutic Medicine, 18, 1039-1046. https://doi.org/10.3892/etm.2019.7659
MLA
Jia, S., Zhang, S., Wang, X., Yang, Z., Sun, Y., Gupta, A., Hou, R., Lei, D., Hu, K., Ye, W., Wang, L."Calcitonin gene‑related peptide enhances osteogenic differentiation and recruitment of bone marrow mesenchymal stem cells in rats". Experimental and Therapeutic Medicine 18.2 (2019): 1039-1046.
Chicago
Jia, S., Zhang, S., Wang, X., Yang, Z., Sun, Y., Gupta, A., Hou, R., Lei, D., Hu, K., Ye, W., Wang, L."Calcitonin gene‑related peptide enhances osteogenic differentiation and recruitment of bone marrow mesenchymal stem cells in rats". Experimental and Therapeutic Medicine 18, no. 2 (2019): 1039-1046. https://doi.org/10.3892/etm.2019.7659
Copy and paste a formatted citation
x
Spandidos Publications style
Jia S, Zhang SJ, Wang XD, Yang ZH, Sun YN, Gupta A, Hou R, Lei DL, Hu KJ, Ye WM, Ye WM, et al: Calcitonin gene‑related peptide enhances osteogenic differentiation and recruitment of bone marrow mesenchymal stem cells in rats. Exp Ther Med 18: 1039-1046, 2019.
APA
Jia, S., Zhang, S., Wang, X., Yang, Z., Sun, Y., Gupta, A. ... Wang, L. (2019). Calcitonin gene‑related peptide enhances osteogenic differentiation and recruitment of bone marrow mesenchymal stem cells in rats. Experimental and Therapeutic Medicine, 18, 1039-1046. https://doi.org/10.3892/etm.2019.7659
MLA
Jia, S., Zhang, S., Wang, X., Yang, Z., Sun, Y., Gupta, A., Hou, R., Lei, D., Hu, K., Ye, W., Wang, L."Calcitonin gene‑related peptide enhances osteogenic differentiation and recruitment of bone marrow mesenchymal stem cells in rats". Experimental and Therapeutic Medicine 18.2 (2019): 1039-1046.
Chicago
Jia, S., Zhang, S., Wang, X., Yang, Z., Sun, Y., Gupta, A., Hou, R., Lei, D., Hu, K., Ye, W., Wang, L."Calcitonin gene‑related peptide enhances osteogenic differentiation and recruitment of bone marrow mesenchymal stem cells in rats". Experimental and Therapeutic Medicine 18, no. 2 (2019): 1039-1046. https://doi.org/10.3892/etm.2019.7659
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team