Open Access

Calcitonin gene‑related peptide enhances osteogenic differentiation and recruitment of bone marrow mesenchymal stem cells in rats

  • Authors:
    • Sen Jia
    • Shi‑Jian Zhang
    • Xu‑Dong Wang
    • Zi‑Hui Yang
    • Ya‑Nan Sun
    • Anand Gupta
    • Rui Hou
    • De‑Lin Lei
    • Kai‑Jin Hu
    • Wei‑Min Ye
    • Lei Wang
  • View Affiliations

  • Published online on: June 11, 2019     https://doi.org/10.3892/etm.2019.7659
  • Pages: 1039-1046
  • Copyright: © Jia et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study evaluated the effects of calcitonin gene‑related peptide (CGRP) on bone marrow mesenchymal stem cells (BMMSCs) in vitro and in a rat model of mandibular distraction osteogenesis (MDO). Rat BMMSCs were isolated then treated with CGRP or CGRP antagonist (CGRP8‑37). The proliferation and migration ability of BMMSCs was determined using 5‑bromo‑2'‑deoxyuridine and Transwell assays, respectively. Osteogenic‑related gene expression was analyzed with reverse transcription‑quantitative polymerase chain reaction. For the in vivo analysis, thirty MDO rats were randomly assigned to control, CGRP or CGRP8‑37 groups. To evaluate the mobilization of BMMSCs, nestin and stromal cell‑derived factor 1 (SDF‑1) were detected by immunohistochemistry and ELISA. Rats were sacrificed following 14 days and new bone formation was assessed by histological and micro‑computed tomography analysis. In the in vitro results, the CGRP group demonstrated significantly higher migration and proliferation, as well as enhanced alkaline phosphatase and runt‑related transcription factor 2 expression compared with the control. In the in vivo experiments, bone mineral density of the newly formed bone in the CGRP group was significantly higher than controls. The nestin and SDF‑1 expression in the CGRP group was also significantly upregulated. In conclusion, the present study demonstrated that CGRP administration increased new bone formation, possibly via enhancing BMMSC migration and differentiation in MDO rats.
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August-2019
Volume 18 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Jia S, Zhang SJ, Wang XD, Yang ZH, Sun YN, Gupta A, Hou R, Lei DL, Hu KJ, Ye WM, Ye WM, et al: Calcitonin gene‑related peptide enhances osteogenic differentiation and recruitment of bone marrow mesenchymal stem cells in rats. Exp Ther Med 18: 1039-1046, 2019
APA
Jia, S., Zhang, S., Wang, X., Yang, Z., Sun, Y., Gupta, A. ... Wang, L. (2019). Calcitonin gene‑related peptide enhances osteogenic differentiation and recruitment of bone marrow mesenchymal stem cells in rats. Experimental and Therapeutic Medicine, 18, 1039-1046. https://doi.org/10.3892/etm.2019.7659
MLA
Jia, S., Zhang, S., Wang, X., Yang, Z., Sun, Y., Gupta, A., Hou, R., Lei, D., Hu, K., Ye, W., Wang, L."Calcitonin gene‑related peptide enhances osteogenic differentiation and recruitment of bone marrow mesenchymal stem cells in rats". Experimental and Therapeutic Medicine 18.2 (2019): 1039-1046.
Chicago
Jia, S., Zhang, S., Wang, X., Yang, Z., Sun, Y., Gupta, A., Hou, R., Lei, D., Hu, K., Ye, W., Wang, L."Calcitonin gene‑related peptide enhances osteogenic differentiation and recruitment of bone marrow mesenchymal stem cells in rats". Experimental and Therapeutic Medicine 18, no. 2 (2019): 1039-1046. https://doi.org/10.3892/etm.2019.7659