Human hepatocellular adenoma as a promising cell source for bioartificial liver systems

  • Authors:
    • Qun Yan
    • Xin‑Mei Zhao
    • Li‑Juan Deng
    • Yu‑Xin Fang
    • Jian‑Jiao Lin
    • Ai‑Min Li
  • View Affiliations

  • Published online on: June 13, 2019     https://doi.org/10.3892/etm.2019.7673
  • Pages: 1357-1365
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Abstract

The present study assessed human hepatocellular adenoma (HCA) as a potential source of biological material for bioartificial liver (BAL) systems. The histological characteristics of HCA tissues from 8 patients were examined using hematoxylin and eosin staining. The glycogen synthesis capacity of HCA cells was assessed using Periodic Acid‑Schiff (PAS) staining and the expression of genes involved in liver function were examined using immunohistochemical staining (IHC) and reverse transcription‑quantitative PCR analysis. Primary cells from HCA tissues were subsequently isolated and cultured in vitro. Cells within HCA tissues from 8 patients exhibited a polygonal shape, similar to that of cells in adjacent normal liver tissues. PAS staining of HCA tissues indicated the capacity of these cells to synthesize and store glycogen. Furthermore, IHC and PCR analyses revealed that the expression of liver function genes in HCA tissues were similar to those observed within normal adjacent liver tissues. Primary cells isolated from HCA tissues exhibited an irregular polygonal shape and positive in vitro growth. The current study demonstrated that HCA tissues exhibit histological and functional characteristics matching those of normal human liver tissue and may therefore be a promising alternative to hepatocytes as a source of biological material for BAL systems.
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August-2019
Volume 18 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Yan Q, Zhao XM, Deng LJ, Fang YX, Lin JJ and Li AM: Human hepatocellular adenoma as a promising cell source for bioartificial liver systems. Exp Ther Med 18: 1357-1365, 2019
APA
Yan, Q., Zhao, X., Deng, L., Fang, Y., Lin, J., & Li, A. (2019). Human hepatocellular adenoma as a promising cell source for bioartificial liver systems. Experimental and Therapeutic Medicine, 18, 1357-1365. https://doi.org/10.3892/etm.2019.7673
MLA
Yan, Q., Zhao, X., Deng, L., Fang, Y., Lin, J., Li, A."Human hepatocellular adenoma as a promising cell source for bioartificial liver systems". Experimental and Therapeutic Medicine 18.2 (2019): 1357-1365.
Chicago
Yan, Q., Zhao, X., Deng, L., Fang, Y., Lin, J., Li, A."Human hepatocellular adenoma as a promising cell source for bioartificial liver systems". Experimental and Therapeutic Medicine 18, no. 2 (2019): 1357-1365. https://doi.org/10.3892/etm.2019.7673