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Article

Preparation of mouse anti‑human rotavirus VP7 monoclonal antibody and its protective effect on rotavirus infection

  • Authors:
    • Mei Zha
    • Jing Yang
    • Linlin Zhou
    • Hongren Wang
    • Xing Pan
    • Zhaomin Deng
    • Yuan Yang
    • Wanyi Li
    • Baoning Wang
    • Mingyuan Li
  • View Affiliations / Copyright

    Affiliations: Department of Microbiology, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Institute of Liver Diseases, Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China, Institute of Infection and Immunity, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China, Hospital of Chengdu Office of People's Government of Tibetan Autonomous Region, Chengdu, Sichuan 610041, P.R. China
  • Pages: 1384-1390
    |
    Published online on: June 25, 2019
       https://doi.org/10.3892/etm.2019.7708
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Abstract

The aim of the current study was to prepare and identify mouse anti‑human rotavirus (RV) VP7 monoclonal antibodies and explore their protective effects on RV infection. The mouse anti‑human RV VP7 monoclonal antibody was produced using the ascites method and identified via western blot analysis. In vitro neutralization of mouse anti‑human RV VP7 monoclonal antibodies was detected by performing an MTT assay. The TCID50 value was calculated to obtain antibody neutralization titers. A mouse RV infection model was generated to assess the protective effect of the mouse anti‑human RV VP7 monoclonal antibody in experimental animals. Monoclonal antibodies were successfully prepared and their purity reached ≥90%. Western blotting demonstrated that monoclonal antibodies specifically bound to the purified Wa RV strain, with a specific reaction band at ~40 kDa. Monoclonal antibody in vitro neutralization results demonstrated that cell survival rate in the virus + monoclonal antibody group was higher than that in virus + maintenance fluid group (P<0.05). Monoclonal antibody neutralization titer detection revealed that the cytopathic effect did not extend beyond 4 days. In addition, the calculated monoclonal antibody neutralization titer was 1:446. The results revealed that the positive rate of colloidal gold RV in the 100 µl monoclonal antibody group was significantly lower than that in the control group (P<0.05). Furthermore, the protection rate of the 100 µl monoclonal antibody group was 71.4%, whereas the 50 µl monoclonal antibody group was 42.9% and the ribavirin group was 57.1%. In conclusion, the results of the current study demonstrated that mouse anti‑human RV VP7 monoclonal antibodies can be successfully prepared using ascites method. These antibodies also effectively neutralize the cytotoxic effects of the human RV Wa strain in vitro and mouse anti‑human RV VP7 monoclonal antibodies also exhibited a good protective role in mice. Furthermore, greater protective effects were observed at a higher dose and the protective effects of these high dose treatments were superior to that of ribavirin.
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Copy and paste a formatted citation
Spandidos Publications style
Zha M, Yang J, Zhou L, Wang H, Pan X, Deng Z, Yang Y, Li W, Wang B, Li M, Li M, et al: Preparation of mouse anti‑human rotavirus VP7 monoclonal antibody and its protective effect on rotavirus infection. Exp Ther Med 18: 1384-1390, 2019.
APA
Zha, M., Yang, J., Zhou, L., Wang, H., Pan, X., Deng, Z. ... Li, M. (2019). Preparation of mouse anti‑human rotavirus VP7 monoclonal antibody and its protective effect on rotavirus infection. Experimental and Therapeutic Medicine, 18, 1384-1390. https://doi.org/10.3892/etm.2019.7708
MLA
Zha, M., Yang, J., Zhou, L., Wang, H., Pan, X., Deng, Z., Yang, Y., Li, W., Wang, B., Li, M."Preparation of mouse anti‑human rotavirus VP7 monoclonal antibody and its protective effect on rotavirus infection". Experimental and Therapeutic Medicine 18.2 (2019): 1384-1390.
Chicago
Zha, M., Yang, J., Zhou, L., Wang, H., Pan, X., Deng, Z., Yang, Y., Li, W., Wang, B., Li, M."Preparation of mouse anti‑human rotavirus VP7 monoclonal antibody and its protective effect on rotavirus infection". Experimental and Therapeutic Medicine 18, no. 2 (2019): 1384-1390. https://doi.org/10.3892/etm.2019.7708
Copy and paste a formatted citation
x
Spandidos Publications style
Zha M, Yang J, Zhou L, Wang H, Pan X, Deng Z, Yang Y, Li W, Wang B, Li M, Li M, et al: Preparation of mouse anti‑human rotavirus VP7 monoclonal antibody and its protective effect on rotavirus infection. Exp Ther Med 18: 1384-1390, 2019.
APA
Zha, M., Yang, J., Zhou, L., Wang, H., Pan, X., Deng, Z. ... Li, M. (2019). Preparation of mouse anti‑human rotavirus VP7 monoclonal antibody and its protective effect on rotavirus infection. Experimental and Therapeutic Medicine, 18, 1384-1390. https://doi.org/10.3892/etm.2019.7708
MLA
Zha, M., Yang, J., Zhou, L., Wang, H., Pan, X., Deng, Z., Yang, Y., Li, W., Wang, B., Li, M."Preparation of mouse anti‑human rotavirus VP7 monoclonal antibody and its protective effect on rotavirus infection". Experimental and Therapeutic Medicine 18.2 (2019): 1384-1390.
Chicago
Zha, M., Yang, J., Zhou, L., Wang, H., Pan, X., Deng, Z., Yang, Y., Li, W., Wang, B., Li, M."Preparation of mouse anti‑human rotavirus VP7 monoclonal antibody and its protective effect on rotavirus infection". Experimental and Therapeutic Medicine 18, no. 2 (2019): 1384-1390. https://doi.org/10.3892/etm.2019.7708
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