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MicroRNA‑466 inhibits cancer cell migration and invasion in hepatocellular carcinoma by indirectly mediating the downregulation of ROCK2

  • Authors:
    • Ning Ai
    • Bo Li
    • Li Li
    • Zhigang Li
    • Hong Ji
    • Guang Yang
    • Fenghua Yin
  • View Affiliations / Copyright

    Affiliations: Department of Interventional Radiology, The Fourth Hospital of Hebei Medical University, Shijiangzhuang, Hebei 050011, P.R. China, Department of General Surgery, The Second Hospital of Hebei Medical University, Shijiangzhuang, Hebei 050011, P.R. China
    Copyright: © Ai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1493-1499
    |
    Published online on: June 25, 2019
       https://doi.org/10.3892/etm.2019.7709
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Abstract

MicroRNA‑466 was recently characterized as a tumor suppressor with known biological function in prostate cancer. The aim of the current study was to investigate the possible involvement of microRNA‑466 in hepatocellular carcinoma (HCC). The current study demonstrated that the expression level of microRNA‑466 was significantly downregulated; while the mRNA expression level of Rho‑associated coiled‑coil containing protein kinase 2 (ROCK2) was significantly upregulated in tumor tissue compared with adjacent healthy tissue samples obtained from patients with HCC. In addition, the relative plasma level of microRNA‑466 was significantly decreased, while the relative plasma level of ROCK2 was significantly increased in patients with HCC compared with healthy controls. Expression levels of microRNA‑466 and ROCK2 were inversely correlated in tumor tissue but not in adjacent healthy tissue samples obtained from patients with HCC. Plasma levels of microRNA‑466 and ROCK2 were inversely correlated in patients with HCC but not in healthy controls. In addition, reduced plasma levels of microRNA‑466 may have a diagnostic value in the detection of early stage HCC. MicroRNA‑466 overexpression significantly suppressed ROCK2 expression in HCC cells, whereas ROCK2 overexpression did not significantly affect microRNA‑466 expression. MicroRNA‑466 overexpression significantly suppressed, while ROCK2 overexpression significantly enhanced HCC cell migration and invasion. In addition, ROCK2 overexpression partially reversed the inhibitory effect of microRNA‑466 overexpression on HCC cell migration and invasion. Taken together, these results suggest that microRNA‑466 may inhibit HCC cell migration and invasion by indirectly mediating the downregulation of ROCK2.
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1 

Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J and Jemal A: Global cancer statistics, 2012. CA Cancer J Clin. 65:87–108. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Chen W, Zheng R, Baade PD, Zhang S, Zeng H, Bray F, Jemal A, Yu XQ and He J: Cancer statistics in China, 2015. CA Cancer J Clin. 66:115–132. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Kudo M: Surveillance, diagnosis, treatment, and outcome of liver cancer in Japan. Liver Cancer. 4:39–50. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Gao YY, Chen H, Zhou YY, Wang LT, Hou Y, Xia XH and Ding Y: Intraorgan targeting of gold conjugates for precise liver cancer treatment. ACS Appl Mater Interfaces. 9:31458–31468. 2017. View Article : Google Scholar : PubMed/NCBI

5 

Bosch FX, Ribes J, Díaz M and Cléries R: Primary liver cancer: Worldwide incidence and trends. Gastroenterology 127 (5 Suppl 1). S5–S16. 2004.

6 

Palmer WC and Patel T: Are common factors involved in the pathogenesis of primary liver cancers? A meta-analysis of risk factors for intrahepatic cholangiocarcinoma. J Hepatol. 57:69–76. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Vigil D, Kim TY, Plachco A, Garton AJ, Castaldo L, Pachter JA, Dong H, Chen X, Tokar B, Campbell SL and Der CJ: ROCK1 and ROCK2 are required for non-small cell lung cancer anchorage-independent growth and invasion. Cancer Res. 72:5338–5347. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Li M, Ke J, Wang Q, Qian H, Yang L, Zhang X, Xiao J, Ding H, Shan X, Liu Q, et al: Upregulation of ROCK2 in gastric cancer cell promotes tumor cell proliferation, metastasis and invasion. Clin Exp Med. 17:519–529. 2017. View Article : Google Scholar : PubMed/NCBI

9 

Zheng F, Liao YJ, Cai MY, Liu YH, Liu TH, Chen SP, Bian XW, Guan XY, Lin MC, Zeng YX, et al: The putative tumour suppressor microRNA-124 modulates hepatocellular carcinoma cell aggressiveness by repressing ROCK2 and EZH2J. Gut. 61:278–289. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Rath N and Olson MF: Rho-associated kinases in tumorigenesis: Re-considering ROCK inhibition for cancer therapy. EMBO Rep. 13:900–908. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Kroiss A, Vincent S, Decaussin-Petrucci M, Meugnier E, Viallet J, Ruffion A, Chalmel F, Samarut J and Allioli N: Androgen-regulated microRNA-135a decreases prostate cancer cell migration and invasion through downregulating ROCK1 and ROCK2. Oncogene. 34:2846–2855. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Peng F, Jiang J, Yu Y, Tian R, Guo X, Li X, Shen M, Xu M, Zhu F, Shi C, et al: Direct targeting of SUZ12/ROCK2 by miR-200b/c inhibits cholangiocarcinoma tumourigenesis and metastasis. Br J Cancer. 109:3092–3104. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Colden M, Dar AA, Saini S, Dahiya PV, Shahryari V, Yamamura S, Tanaka Y, Stein G, Dahiya R and Majid S: MicroRNA-466 inhibits tumor growth and bone metastasis in prostate cancer by direct regulation of osteogenic transcription factor RUNX2. Cell Death Dis. 8:e25722017. View Article : Google Scholar : PubMed/NCBI

14 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

15 

Ji J, Shi J, Budhu A, Yu Z, Forgues M, Roessler S, Ambs S, Chen Y, Meltzer PS, Croce CM, et al: MicroRNA expression, survival, and response to interferon in liver cancer. N Engl J Med. 361:1437–1447. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Huang S and He X: The role of microRNAs in liver cancer progression. Br J Cancer. 104:235–240. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Lepage C, Capocaccia R, Hackl M, Lemmens V, Molina E, Pierannunzio D, Sant M, Trama A and Faivre J; EUROCARE-5 Working Group, : Survival in patients with primary liver cancer, gallbladder and extrahepatic biliary tract cancer and pancreatic cancer in Europe 1999–2007: Results of EUROCARE-5. Eur J Cancer. 51:2169–2178. 2015. View Article : Google Scholar : PubMed/NCBI

18 

Dourado MR, de Oliveira CE, Sawazaki-Calone I, Sundquist E, Coletta RD and Salo T: Clinicopathologic significance of ROCK2 expression in oral squamous cell carcinomas. J Oral Pathol Med. 47:121–127. 2018. View Article : Google Scholar : PubMed/NCBI

19 

Xu Z, Hong Z, Ma M, Liu X, Chen L, Zheng C, Xi X and Shao J: Rock2 promotes RCC proliferation by decreasing SCARA5 expression through β-catenin/TCF4 signaling. Biochem Biophys Res Commun. 480:586–593. 2016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Ai N, Li B, Li L, Li Z, Ji H, Yang G and Yin F: MicroRNA‑466 inhibits cancer cell migration and invasion in hepatocellular carcinoma by indirectly mediating the downregulation of ROCK2. Exp Ther Med 18: 1493-1499, 2019.
APA
Ai, N., Li, B., Li, L., Li, Z., Ji, H., Yang, G., & Yin, F. (2019). MicroRNA‑466 inhibits cancer cell migration and invasion in hepatocellular carcinoma by indirectly mediating the downregulation of ROCK2. Experimental and Therapeutic Medicine, 18, 1493-1499. https://doi.org/10.3892/etm.2019.7709
MLA
Ai, N., Li, B., Li, L., Li, Z., Ji, H., Yang, G., Yin, F."MicroRNA‑466 inhibits cancer cell migration and invasion in hepatocellular carcinoma by indirectly mediating the downregulation of ROCK2". Experimental and Therapeutic Medicine 18.2 (2019): 1493-1499.
Chicago
Ai, N., Li, B., Li, L., Li, Z., Ji, H., Yang, G., Yin, F."MicroRNA‑466 inhibits cancer cell migration and invasion in hepatocellular carcinoma by indirectly mediating the downregulation of ROCK2". Experimental and Therapeutic Medicine 18, no. 2 (2019): 1493-1499. https://doi.org/10.3892/etm.2019.7709
Copy and paste a formatted citation
x
Spandidos Publications style
Ai N, Li B, Li L, Li Z, Ji H, Yang G and Yin F: MicroRNA‑466 inhibits cancer cell migration and invasion in hepatocellular carcinoma by indirectly mediating the downregulation of ROCK2. Exp Ther Med 18: 1493-1499, 2019.
APA
Ai, N., Li, B., Li, L., Li, Z., Ji, H., Yang, G., & Yin, F. (2019). MicroRNA‑466 inhibits cancer cell migration and invasion in hepatocellular carcinoma by indirectly mediating the downregulation of ROCK2. Experimental and Therapeutic Medicine, 18, 1493-1499. https://doi.org/10.3892/etm.2019.7709
MLA
Ai, N., Li, B., Li, L., Li, Z., Ji, H., Yang, G., Yin, F."MicroRNA‑466 inhibits cancer cell migration and invasion in hepatocellular carcinoma by indirectly mediating the downregulation of ROCK2". Experimental and Therapeutic Medicine 18.2 (2019): 1493-1499.
Chicago
Ai, N., Li, B., Li, L., Li, Z., Ji, H., Yang, G., Yin, F."MicroRNA‑466 inhibits cancer cell migration and invasion in hepatocellular carcinoma by indirectly mediating the downregulation of ROCK2". Experimental and Therapeutic Medicine 18, no. 2 (2019): 1493-1499. https://doi.org/10.3892/etm.2019.7709
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