Investigation into the expression levels of MAGEA6 in esophageal squamous cell carcinoma and esophageal adenocarcinoma tissues

Hao,Jun; Li,Shuying; Li,Jintao; Jiang,Zhu; Ghaffar,Maliha; Wang,Minglian; Jia,Runqing; Chen,Su; Wang,Yangjunqi; Zeng,Yi

doi:10.3892/etm.2019.7735

Investigation into the expression levels of MAGEA6 in esophageal squamous cell carcinoma and esophageal adenocarcinoma tissues

Authors:
- Jun Hao
- Shuying Li
- Jintao Li
- Zhu Jiang
- Maliha Ghaffar
- Minglian Wang
- Runqing Jia
- Su Chen
- Yangjunqi Wang
- Yi Zeng
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Affiliations: Beijing Key Laboratory of Environmental and Viral Oncology, College of Life Science and Bio‑Engineering, Beijing University of Technology, Beijing 100124, P.R. China, Hebei Key Laboratory for Chronic Diseases, Tangshan Key Laboratory for Preclinical and Basic Research on Chronic Diseases, North China University of Science and Technology, Tangshan, Hebei 063000, P.R. China, Hubei Key Laboratory of Medical Information Analysis and Tumor Diagnosis and Treatment, Wuhan, Hubei 430074, P.R. China
Published online on: July 5, 2019 https://doi.org/10.3892/etm.2019.7735
Pages: 1816-1822

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Abstract

Esophageal carcinoma (EC) is the sixth most deadly of all cancers. It is among the most malignant cancers due to its highly aggressive nature and low survival rate. The incidence of EC is high in Asia, particularly in Southern areas including China, Iran and Japan. There is a large body of evidence to suggest an association between the melanoma antigen gene (MAGE) family and the initiation of cancer; however, there is no clear evidence to suggest an association between EC and MAGE. Discovery of the chemical and physiological processes relevant to the occurrence of EC is vital for clinicians to diagnose and treat this highly aggressive cancer. The present study focused on the association of EC with the expression of MAGE family member A6 (MAGEA6) at the mRNA and protein levels using gene chip, reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and immunohistochemistry. The expression of MAGEA6 in human esophageal squamous cell carcinoma (ESCC) and esophageal adenocarcinoma (EAC) tissue samples were compared with those in paracancerous tissue. The result of the gene chip assay revealed that as the generation grew, there was a significant increase in MAGEA6 transcription in the esophageal epithelial cell line, SHEE Different ESC cell lines also exhibited a significantly higher transcription of MAGEA6 compared with the HaCaT cell line, as determined via reverse transcription‑quantitative PCR. An higher positive rate of MAGEA6 expression in ESCC and EAC tissues was also revealed when compared with paracancerous tissues, as determined via immunohistochemistry. The results indicated that MAGEA6 is highly transcribed and expressed in the development of EC and may therefore serve as a novel biomarker for the diagnosis or treatment of EC.

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