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MALAT1 overexpression promotes the proliferation of human periodontal ligament stem cells by upregulating fibroblast growth factor 2

  • Authors:
    • Pei Chen
    • Yanhong Huang
    • Yarong Wang
    • Shaobing Li
    • Hongxing Chu
    • Mingdeng Rong
  • View Affiliations / Copyright

    Affiliations: Department of Periodontology and Implantology, Stomatological Hospital, Southern Medical University, Guangzhou, Guangdong 510280, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1627-1632
    |
    Published online on: July 8, 2019
       https://doi.org/10.3892/etm.2019.7748
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Abstract

Fibroblast growth factor 2 (FGF2) has been revealed to promote human periodontal ligament stem cell (PDLSC) proliferation. The abnormal proliferation of PDLSCs has also been associated with the pathogenesis of periodontitis. The long non‑coding RNA, metastasis‑associated lung adenocarcinoma transcript 1 (MALAT1), has been demonstrated to regulate FGF2 secretion. Therefore, MALAT1 may also be associated with periodontitis. The aim of the present study was to investigate the effect of MALAT1 overexpression on the proliferation of PDLSCs. In the current study, PDLSCs derived from healthy and periodontitis‑affected teeth were collected. MALAT1 and FGF2 mRNA expression in PDLSCs was detected using reverse transcription‑quantitative PCR. PDLSCs overexpressing MALAT1 were subsequently generated. PDLSC proliferation was analyzed using a Cell Counting kit‑8 assay. FGF2 protein expression was detected using western blot analysis. The results revealed that MALAT1 and FGF2 mRNA were significantly upregulated in PDLSCs derived from periodontitis‑affected teeth when compared with PDLSCs derived from healthy teeth. PDLSCs derived from periodontitis‑affected teeth also demonstrated a significantly higher proliferation rate than PDLSCs derived from healthy teeth. MALAT1 and FGF2 mRNA expression were positively correlated in both PDLSC groups. MALAT1 overexpression promoted the proliferation of healthy and periodontitis‑affected PDLSC groups and upregulated FGF2 protein expression. The present study concluded that MALAT1 overexpression promoted the proliferation of human PDLSC potentially via upregulating FGF2.
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View References

1 

Hajishengallis G: Periodontitis: From microbial immune subversion to systemic. Inflammation. Nat Rev Immunol. 15:30–44. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Lundberg K, Wegner N, Yucel-Lindberg T and Venables PJ: Periodontitis in RA-the citrullinated enolase connection. Nat Rev Rheumatol. 6:727–730. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Kebschull M, Demmer RT and Papapanou PN: ‘Gum bug, leave my heart alone!’-epidemiologic and mechanistic evidence linking periodontal infections and atherosclerosis. J Dent Res. 89:879–902. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Madianos PN, Bobetsis YA and Offenbacher S: Adverse pregnancy outcomes (APOs) and periodontal disease: pathogenic mechanisms. J Periodontol. 84 (Suppl):S170–S180. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Zhang Q, Li Z, Wang C, Shen T, Yang Y, Chotivichien S and Wang L: Prevalence and predictors for periodontitis among adults in China, 2010. Glob Health Action. 7:245032014. View Article : Google Scholar : PubMed/NCBI

6 

Cionca N: Use and misuse of systemic antibiotics in periodontitis treatment. Oral Health Prev Dent. 15:305–306. 2017.PubMed/NCBI

7 

Zheng W, Wang S, Wang J and Jin F: Periodontitis promotes the proliferation and suppresses the differentiation potential of human periodontal ligament stem cells. Int J Mol Med. 36:915–922. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Lee JH, Um S, Jang JH and Seo BM: Effects of VEGF and FGF-2 on proliferation and differentiation of human periodontal ligament stem cells. Cell Tissue Res. 348:475–484. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Huang JK, Ma L, Song WH, Lu BY, Huang YB, Dong HM, Ma XK, Zhu ZZ and Zhou R: LncRNA-MALAT1 promotes angiogenesis of thyroid cancer by modulating tumor-associated macrophage FGF2 protein secretion. J Cell Biochem. 118:4821–4830. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Liu JH, Chen G, Dang YW, Li CJ and Luo DZ: Expression and prognostic significance of lncRNA MALAT1 in pancreatic cancer tissues. Asian Pac J Cancer Prev. 15:2971–2977. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Tian X and Xu G: Clinical value of lncRNA MALAT1 as a prognostic marker in human cancer: Systematic review and meta-analysis. BMJ Open. 5:e0086532015. View Article : Google Scholar : PubMed/NCBI

12 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

13 

Zou Y, Li C, Shu F, Tian Z, Xu W, Xu H, Tian H, Shi R and Mao X: lncRNA expression signatures in periodontitis revealed by microarray: The potential role of lncRNAs in periodontitis pathogenesis. J Cell Biochem. 116:640–647. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Wang L, Wu F, Song Y, Li X, Wu Q, Duan Y and Jin Z: Long noncoding RNA related to periodontitis interacts with miR-182 to upregulate osteogenic differentiation in periodontal mesenchymal stem cells of periodontitis patients. Cell Tissue Res. 7:e23272016.

15 

Bochenek G, Häsler R, El Mokhtari NE, König IR, Loos BG, Jepsen S, Rosenstiel P, Schreiber S and Schaefer AS: The large non-coding RNA ANRIL, which is associated with atherosclerosis, periodontitis and several forms of cancer, regulates ADIPOR1, VAMP3 and C11ORF10. Hum Mol Genet. 22:4516–4527. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Kitamura M, Akamatsu M, Machigashira M, Hara Y, Sakagami R, Hirofuji T, Hamachi T, Maeda K, Yokota M, Kido J, et al: FGF-2 stimulates periodontal regeneration: results of a multi-center randomized clinical trial. J Dent Res. 90:35–40. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Murakami S: Periodontal tissue regeneration by signaling molecule(s): What role does basic fibroblast growth factor (FGF-2) have in periodontal therapy? Periodontol. 56:188–208. 2000. View Article : Google Scholar

18 

Lu W, Huang SY, Su L, Zhao BX and Miao JY: Long noncoding RNA LOC100129973 suppresses apoptosis by targeting miR-4707-5p and miR-4767 in vascular endothelial cells. Sci Rep. 6:216202016. View Article : Google Scholar : PubMed/NCBI

19 

Andrukhov O, Hong JS, Andrukhova O, Blufstein A, Moritz A and Rausch-Fan X: Response of human periodontal ligament stem cells to IFN-γ and TLR-agonists. Sci Rep. 7:128562017. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Chen P, Huang Y, Wang Y, Li S, Chu H and Rong M: MALAT1 overexpression promotes the proliferation of human periodontal ligament stem cells by upregulating fibroblast growth factor 2. Exp Ther Med 18: 1627-1632, 2019.
APA
Chen, P., Huang, Y., Wang, Y., Li, S., Chu, H., & Rong, M. (2019). MALAT1 overexpression promotes the proliferation of human periodontal ligament stem cells by upregulating fibroblast growth factor 2. Experimental and Therapeutic Medicine, 18, 1627-1632. https://doi.org/10.3892/etm.2019.7748
MLA
Chen, P., Huang, Y., Wang, Y., Li, S., Chu, H., Rong, M."MALAT1 overexpression promotes the proliferation of human periodontal ligament stem cells by upregulating fibroblast growth factor 2". Experimental and Therapeutic Medicine 18.3 (2019): 1627-1632.
Chicago
Chen, P., Huang, Y., Wang, Y., Li, S., Chu, H., Rong, M."MALAT1 overexpression promotes the proliferation of human periodontal ligament stem cells by upregulating fibroblast growth factor 2". Experimental and Therapeutic Medicine 18, no. 3 (2019): 1627-1632. https://doi.org/10.3892/etm.2019.7748
Copy and paste a formatted citation
x
Spandidos Publications style
Chen P, Huang Y, Wang Y, Li S, Chu H and Rong M: MALAT1 overexpression promotes the proliferation of human periodontal ligament stem cells by upregulating fibroblast growth factor 2. Exp Ther Med 18: 1627-1632, 2019.
APA
Chen, P., Huang, Y., Wang, Y., Li, S., Chu, H., & Rong, M. (2019). MALAT1 overexpression promotes the proliferation of human periodontal ligament stem cells by upregulating fibroblast growth factor 2. Experimental and Therapeutic Medicine, 18, 1627-1632. https://doi.org/10.3892/etm.2019.7748
MLA
Chen, P., Huang, Y., Wang, Y., Li, S., Chu, H., Rong, M."MALAT1 overexpression promotes the proliferation of human periodontal ligament stem cells by upregulating fibroblast growth factor 2". Experimental and Therapeutic Medicine 18.3 (2019): 1627-1632.
Chicago
Chen, P., Huang, Y., Wang, Y., Li, S., Chu, H., Rong, M."MALAT1 overexpression promotes the proliferation of human periodontal ligament stem cells by upregulating fibroblast growth factor 2". Experimental and Therapeutic Medicine 18, no. 3 (2019): 1627-1632. https://doi.org/10.3892/etm.2019.7748
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