Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
September-2019 Volume 18 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September-2019 Volume 18 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Function and mechanism of pyrin and IL‑10 in the regulation of the inflammasome in pulmonary vascular endothelial cells following hemorrhagic shock

  • Authors:
    • Xin Jin
    • Yongxing Yao
    • Xing Lu
    • Peng Xu
    • Yanfei Xia
    • Shengmei Zhu
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesia, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310003, P.R. China, Department of Anesthesia, Zhejiang Hospital, Hangzhou, Zhejiang 310013, P.R. China
    Copyright: © Jin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1768-1774
    |
    Published online on: July 9, 2019
       https://doi.org/10.3892/etm.2019.7757
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The present study aimed to evaluate the function of pyrin and interleukin‑10 (IL‑10) and the potential mechanisms underlying the regulation of inflammation in pulmonary vascular endothelial cells (ECs) following hemorrhagic shock (HS). Adult female Sprague‑Dawley rats were divided into 4 groups (n=6 in each group) to examine the changes in pyrin expression following HS‑lipopolysaccharide (LPS) administration, including the following groups: A sham operation (SM) + tracheal injection of saline (SAL) group; a HS + SAL group; a SM + LPS group (with a tracheal injection of endotoxin); and a HS + LPS group. An additional 4 groups were used to evaluate the function of IL‑10, by the additional intratracheal injection of recombinant IL‑10. Western blot analysis and immunofluorescence were performed in order to investigate the changes to pyrin and IL‑10 expression in pulmonary vascular ECs. The expression levels of pyrin in the SM + LPS group were significantly increased in comparison with the SM + SAL group (P<0.01). Additionally, the expression levels of pyrin were significantly increased in the HS + LPS group compared with the HS + SAL group (P<0.01). The expression levels of caspase‑1 were significantly increased in the HS + LPS group compared with those in the other three groups (P<0.01). The expression levels of pyrin in the HS + LPS + IL‑10 group were significantly increased compared with the HS + LPS group (P<0.01). The expression levels of caspase‑1 were significantly decreased following IL‑10 treatment compared with those in the HS + LPS group (P<0.01). Therefore, HS attenuated LPS‑induced pyrin expression in pulmonary vascular ECs and may also inhibit the expression of IL‑10, resulting in the activation of caspase‑1 subsequent to a second LPS insult.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

El-Menyar A, El-Thani A, El Rasheid Z, Ahmad Z, Tuma M, AbdulRahman H, Parchani A, Peralta R and Latifi R: Multiple organ dysfunction syndrome (MODS): Is it preventable or inevitable. Int J Clin Med. 3:722–730. 2012. View Article : Google Scholar

2 

van Wessem KJP and Leenen LPH: Reduction in mortality rates of postinjury multiple organ dysfunction syndrome: A shifting paradigm? A prospective population-based cohort study. Shock. 49:33–38. 2018. View Article : Google Scholar : PubMed/NCBI

3 

Anne Morrison C, Moran A, Patel S, Vidaurre Mdel P, Carrick MM and Tweardy DJ: Increased apoptosis of peripheral blood neutrophils is associated with reduced incidence of infection in trauma patients with hemorrhagic shock. J Infect. 66:87–94. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Moore FA, Sauaia A, Moore EE, Haenel JB, Burch JM and Lezotte DC: Postinjury multiple organ failure: A bimodal phenomenon. J Trauma. 40:501–512. 1996. View Article : Google Scholar : PubMed/NCBI

5 

Minei JP, Cuschieri J, Sperry J, Moore EE, West MA, Harbrecht BG, O'Keefe GE, Cohen MJ, Moldawer LL, Tompkins RG, et al: The changing pattern and implications of multiple organ failure after blunt injury with hemorrhagic shock. Crit Care Med. 40:1129–1135. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Li Y, Xiang M, Yuan Y, Xiao G, Zhang J, Jiang Y, Vodovotz Y, Billiar TR, Wilson MA and Fan J: Hemorrhagic shock augments lung endothelial cell activation: Role of temporal alterations of TLR4 and TLR2. Am J Physiol Regul Integr Comp Physiol. 297:R1670–R1680. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Chae JJ, Wood G, Richard K, Jaffe H, Colburn NT, Masters SL, Gumucio DL, Shoham NG and Kastner DL: The familial Mediterranean fever protein, pyrin, is cleaved by caspase-1 and activates NF-kappaB through its N-terminal fragment. Blood. 112:1794–1803. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Chae JJ, Komarow HD, Cheng J, Wood G, Raben N, Liu PP and Kastner DL: Targeted disruption of pyrin, the FMF protein, causes heightened sensitivity to endotoxin and a defect in macrophage apoptosis. Mol Cell. 11:591–604. 2003. View Article : Google Scholar : PubMed/NCBI

9 

He X, Qian Y, Li Z, Fan EK, Li Y, Wu L, Billiar TR, Wilson MA, Shi X and Fan J: TLR4-upregulated IL-1β and IL-1RI promote alveolar macrophage pyroptosis and lung inflammation through an autocrine mechanism. Sci Rep. 6:316632016. View Article : Google Scholar : PubMed/NCBI

10 

Martinon F, Burns K and Tschopp J: The inflammasome: A molecular platform triggering activation of inflammatory caspases and processing of proIL-beta. Mol Cell. 10:417–426. 2002. View Article : Google Scholar : PubMed/NCBI

11 

Suzuki T, Franchi L, Toma C, Ashida H, Ogawa M, Yoshikawa Y, Mimuro H, Inohara N, Sasakawa C and Nuñez G: Differential regulation of caspase-1 activation, pyroptosis, and autophagy via Ipaf and ASC in Shigella-infected macrophages. PLoS Pathog. 3:e1112007. View Article : Google Scholar : PubMed/NCBI

12 

Welborn BM III, Moldawer LL, Seeger JM, Minter RM and Huber TS: Role of endogenous interleukin-10 in local and distant organ injury after visceral ischemia-reperfusion. Shock. 20:35–40. 2003. View Article : Google Scholar : PubMed/NCBI

13 

Molthen RC: A simple, inexpensive, and effective light-carrying laryngoscopic blade for orotracheal intubation of rats. J Am Assoc Lab Anim Sci. 45:88–93. 2006.PubMed/NCBI

14 

Zhu X, Hu H, Li Z, Lin R, Mao J and Chen L: Gua Lou Gui Zhi decoction attenuates post-stroke spasticity via the modulation of GABAB receptors. Mol Med Rep. 12:5957–5962. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Zhou R, Hu DY, Liu LM and Zhou XW: Protective effects of apocynin on ‘two-hit’ injury induced by hemorrhagic shock and lipopolysaccharide. Acta Pharmacol Sin. 23:1023–1028. 2002.PubMed/NCBI

16 

Ulich TR, Fann MJ, Patterson PH, Williams JH, Samal B, Del CJ, Yin S, Guo K and Renick DG: Intratracheal injection of LPS and cytokines. V. LPS induces expression of LIF and LIF inhibits acute inflammation. Am J Physiol. 267:L442–L446. 1994.PubMed/NCBI

17 

Tawadros PS, Powers KA, Yang I, Becker DA, Ginsberg MD, Szaszi K, Kapus A and Rotstein OD: Stilbazulenyl nitrone decreases oxidative stress and reduces lung injury after hemorrhagic shock/resuscitation and LPS. Antioxid Redox Signal. 9:1971–1977. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Testa JE, Chrastina A, Oh P, Li Y, Witkiewicz H, Czarny M, Buss T and Schnitzer JE: Immunotargeting and cloning of two CD34 variants exhibiting restricted expression in adult rat endothelia in vivo. Am J Physiol Lung Cell Mol Physiol. 297:L251–L262. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Feldmeyer L, Keller M, Niklaus G, Hohl D, Werner S and Beer HD: The inflammasome mediates UVB-induced activation and secretion of interleukin-1beta by keratinocytes. Curr Biol. 17:1140–1145. 2007. View Article : Google Scholar : PubMed/NCBI

20 

Martinon F, Hofmann K and Tschopp J: The pyrin domain: A possible member of the death domain-fold family implicated in apoptosis and inflammation. Curr Biol. 11:R118–R120. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Chae J, Cho YH, Lee GS, Cheng J, Liu PP, Feigenbaum L, Katz SI and Kastner DL: Gain-of-function Pyrin mutations induce NLRP3 protein-independent interleukin-1β activation and severe autoinflammation in mice. Immunity. 34:755–768. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Iyer SS and Cheng G: Role of interleukin 10 transcriptional regulation in inflammation and autoimmune disease. Crit Rev Immunol. 32:23–63. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Lo C, Fu M and Cryer HG: Interleukin 10 inhibits alveolar macrophage production of inflammatory mediators involved in adult respiratory distress syndrome. J Surg Res. 79:179–184. 1998. View Article : Google Scholar : PubMed/NCBI

24 

Armstrong L and Millar AB: Relative production of tumour necrosis factor alpha and interleukin 10 in adult respiratory distress syndrome. Thorax. 52:442–446. 1997. View Article : Google Scholar : PubMed/NCBI

25 

Khadaroo RG, Fan J, Powers KA, Fann B, Kapus A and Rotstein OD: Impaired induction of IL-10 expression in the lung following hemorrhagic shock. Shock. 22:333–339. 2004. View Article : Google Scholar : PubMed/NCBI

26 

Yeh FL, Shen HD and Fang RH: Deficient transforming growth factor beta and interleukin-10 responses contribute to the septic death of burned patients. Burns. 28:631–637. 2002. View Article : Google Scholar : PubMed/NCBI

27 

Kusske AM, Rongione AJ, Ashley SW, McFadden DW and Reber HA: Interleukin-10 prevents death in lethal necrotizing pancreatitis in mice. Surgery. 120:284–289. 1996. View Article : Google Scholar : PubMed/NCBI

28 

Cruz CM, Rinna A, Forman HJ, Ventura AL, Persechini PM and Ojcius DM: ATP activates a reactive oxygen species-dependent oxidative stress response and secretion of proinflammatory cytokines in macrophages. J Biol Chem. 282:2871–2879. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Halle A, Hornung V, Petzold GC, Stewart CR, Monks BG, Reinheckel T, Fitzgerald KA, Latz E, Moore KJ and Golenbock DT: The NALP3 inflammasome is involved in the innate immune response to amyloid-beta. Nat Immunol. 9:857–865. 2008. View Article : Google Scholar : PubMed/NCBI

30 

Watanabe H, Gaide O, Pétrilli V, Martinon F, Contassot E, Roques S, Kummer JA, Tschopp J and French LE: Activation of the IL-1beta-processing inflammasome is involved in contact hypersensitivity. J Invest Dermatol. 127:1956–1963. 2007. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Jin X, Yao Y, Lu X, Xu P, Xia Y and Zhu S: Function and mechanism of pyrin and IL‑10 in the regulation of the inflammasome in pulmonary vascular endothelial cells following hemorrhagic shock. Exp Ther Med 18: 1768-1774, 2019.
APA
Jin, X., Yao, Y., Lu, X., Xu, P., Xia, Y., & Zhu, S. (2019). Function and mechanism of pyrin and IL‑10 in the regulation of the inflammasome in pulmonary vascular endothelial cells following hemorrhagic shock. Experimental and Therapeutic Medicine, 18, 1768-1774. https://doi.org/10.3892/etm.2019.7757
MLA
Jin, X., Yao, Y., Lu, X., Xu, P., Xia, Y., Zhu, S."Function and mechanism of pyrin and IL‑10 in the regulation of the inflammasome in pulmonary vascular endothelial cells following hemorrhagic shock". Experimental and Therapeutic Medicine 18.3 (2019): 1768-1774.
Chicago
Jin, X., Yao, Y., Lu, X., Xu, P., Xia, Y., Zhu, S."Function and mechanism of pyrin and IL‑10 in the regulation of the inflammasome in pulmonary vascular endothelial cells following hemorrhagic shock". Experimental and Therapeutic Medicine 18, no. 3 (2019): 1768-1774. https://doi.org/10.3892/etm.2019.7757
Copy and paste a formatted citation
x
Spandidos Publications style
Jin X, Yao Y, Lu X, Xu P, Xia Y and Zhu S: Function and mechanism of pyrin and IL‑10 in the regulation of the inflammasome in pulmonary vascular endothelial cells following hemorrhagic shock. Exp Ther Med 18: 1768-1774, 2019.
APA
Jin, X., Yao, Y., Lu, X., Xu, P., Xia, Y., & Zhu, S. (2019). Function and mechanism of pyrin and IL‑10 in the regulation of the inflammasome in pulmonary vascular endothelial cells following hemorrhagic shock. Experimental and Therapeutic Medicine, 18, 1768-1774. https://doi.org/10.3892/etm.2019.7757
MLA
Jin, X., Yao, Y., Lu, X., Xu, P., Xia, Y., Zhu, S."Function and mechanism of pyrin and IL‑10 in the regulation of the inflammasome in pulmonary vascular endothelial cells following hemorrhagic shock". Experimental and Therapeutic Medicine 18.3 (2019): 1768-1774.
Chicago
Jin, X., Yao, Y., Lu, X., Xu, P., Xia, Y., Zhu, S."Function and mechanism of pyrin and IL‑10 in the regulation of the inflammasome in pulmonary vascular endothelial cells following hemorrhagic shock". Experimental and Therapeutic Medicine 18, no. 3 (2019): 1768-1774. https://doi.org/10.3892/etm.2019.7757
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team