Long non‑coding RNA NEAT1 modulates hypoxia/reoxygenation‑induced cardiomyocyte injury via targeting microRNA‑520a

Wu,Hua‑Jun; Tang,Guan‑Min; Shao,Ping‑Yang; Zou,Hong‑Xing; Shen,Wei‑Feng; Huang,Ming‑De; Pan,Hang‑Hai; Zhai,Chang‑Lin; Qian,Gang

doi:10.3892/etm.2019.7788

Long non‑coding RNA NEAT1 modulates hypoxia/reoxygenation‑induced cardiomyocyte injury via targeting microRNA‑520a

Authors:
- Hua‑Jun Wu
- Guan‑Min Tang
- Ping‑Yang Shao
- Hong‑Xing Zou
- Wei‑Feng Shen
- Ming‑De Huang
- Hang‑Hai Pan
- Chang‑Lin Zhai
- Gang Qian
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Affiliations: Department of Cardiovascular Diseases, The First Affiliated Hospital of Jiaxing University, Jiaxing, Zhejiang 314000, P.R. China
Published online on: July 17, 2019 https://doi.org/10.3892/etm.2019.7788
Pages: 2199-2206

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Abstract

In the present study, a hypoxia/reoxygenation (H/R) model of cardiomyocytes was established to investigate the effects of long non‑coding RNA (LncRNA) Nuclear Enriched Abundant Transcript 1 (NEAT1) and microRNA (miR)‑520a on H/R‑induced cardiomyocyte apoptosis. Flow cytometry and terminal deoxynucleotidyl transferase dUTP nick end labeling staining were used to evaluate cell apoptosis. Luciferase activity assay was used to investigate whether miR‑520a targets NEAT1. Results revealed that NEAT1 was significantly upregulated and miR‑520a was downregulated in the ischemia/reperfusion myocardium and the cardiomyocytes that received H/R treatment. Further study demonstrated that knockdown of NEAT1 and overexpression of miR‑520a serves a protective role against H/R‑induced cardiomyocyte apoptosis. miR‑520a directly targets NEAT1 and its expression level is negatively correlated with that of NEAT1. The findings suggested that NEAT1 and miR‑520a may protect cardiomyocytes from apoptosis through regulating apoptotic proteins B‑cell lymphoma 2 (Bcl‑2) and Bcl‑2‑associated X protein, and altering cleaved caspase3 expression levels.

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